Complete genome analysis of two new bacteriophages isolated
from impetigo strains of Staphylococcus aureus

J 2015

Complete genome analysis of two new bacteriophages isolated from impetigo strains of Staphylococcus aureus

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Hana KONEČNÁ, Roman PANTŮČEK, Ivan RYCHLÍK et. al.

Basic information

Original name

Complete genome analysis of two new bacteriophages isolated from impetigo strains of Staphylococcus aureus

Authors

BOTKA, Tibor (203 Czech Republic, belonging to the institution), Vladislava RŮŽIČKOVÁ (203 Czech Republic, guarantor, belonging to the institution), Hana KONEČNÁ (203 Czech Republic, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Ivan RYCHLÍK (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Petr PETRÁŠ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic, belonging to the institution)

Edition

Virus Genes, Springer US, 2015, 0920-8569

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10607 Virology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.285

RIV identification code

RIV/00216224:14310/15:00080938

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s11262-015-1223-8

UT WoS

000358548500015

Keywords in English

ETA-converting bacteriophages; Staphylococcus aureus; Complete genome sequences;Virion protein patterns

Abstract

V originále

Exfoliative toxin A (ETA)-coding temperate bacteriophages are leading contributors to the toxic phenotype of impetigo strains of Staphylococcus aureus. Two distinct eta gene-positive bacteriophages isolated from S. aureus strains which recently caused massive outbreaks of pemphigus neonatorum in Czech maternity hospitals were characterized. The phages, designated B166 and B236, were able to transfer the eta gene into a prophageless S. aureus strain which afterwards converted into an ETA producer. Complete phage genome sequences were determined, and a comparative analysis of five designed genomic regions revealed major variances between them. They differed in the genome size, number of open reading frames, genome architecture, and virion protein patterns. Their high mutual sequence similarity was detected only in the terminal regions of the genome. When compared with the so far described eta phage genomes, noticeable differences were found. Thus, both phages represent two new lineages of as yet not characterized bacteriophages of the Siphoviridae family having impact on pathogenicity of impetigo strains of S. aureus.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GBP206/12/G151, research and development project

Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice

NT12395, research and development project

Name: Molekulární průkaz a analýza invazivních kmenů small colony variants (SCV) a rezistentních kmenů S. aureus od pacientů s cystickou fibrózou

Files attached

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Citovat

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Hana KONEČNÁ, Roman PANTŮČEK, Ivan RYCHLÍK, Zbyněk ZDRÁHAL, Petr PETRÁŠ and Jiří DOŠKAŘ. Complete genome analysis of two new bacteriophages isolated from impetigo strains of Staphylococcus aureus. Virus Genes. Springer US, 2015, vol. 51, No 1, p. 122-131. ISSN 0920-8569. Available from: https://dx.doi.org/10.1007/s11262-015-1223-8.

@article{1307084,
   author = {Botka, Tibor and Růžičková, Vladislava and Konečná, Hana and Pantůček, Roman and Rychlík, Ivan and Zdráhal, Zbyněk and Petráš, Petr and Doškař, Jiří},
   article_number = {1},
   doi = {http://dx.doi.org/10.1007/s11262-015-1223-8},
   keywords = {ETA-converting bacteriophages; Staphylococcus aureus; Complete genome sequences;Virion protein patterns},
   language = {eng},
   issn = {0920-8569},
   journal = {Virus Genes},
   title = {Complete genome analysis of two new bacteriophages isolated from impetigo strains of Staphylococcus aureus},
   url = {http://link.springer.com/article/10.1007%2Fs11262-015-1223-8},
   volume = {51},
   year = {2015}
}

TY  - JOUR
ID  - 1307084
AU  - Botka, Tibor - Růžičková, Vladislava - Konečná, Hana - Pantůček, Roman - Rychlík, Ivan - Zdráhal, Zbyněk - Petráš, Petr - Doškař, Jiří
PY  - 2015
TI  - Complete genome analysis of two new bacteriophages isolated from impetigo strains of Staphylococcus aureus
JF  - Virus Genes
VL  - 51
IS  - 1
SP  - 122-131
EP  - 122-131
PB  - Springer US
SN  - 09208569
KW  - ETA-converting bacteriophages
KW  - Staphylococcus aureus
KW  - Complete genome sequences;Virion protein patterns
UR  - http://link.springer.com/article/10.1007%2Fs11262-015-1223-8
L2  - http://link.springer.com/article/10.1007%2Fs11262-015-1223-8
N2  - Exfoliative toxin A (ETA)-coding temperate bacteriophages are leading contributors to the toxic phenotype of impetigo strains of Staphylococcus aureus. Two distinct eta gene-positive bacteriophages isolated from S. aureus strains which recently caused massive outbreaks of pemphigus neonatorum in Czech maternity hospitals were characterized. The phages, designated B166 and B236, were able to transfer the eta gene into a prophageless S. aureus strain which afterwards converted into an ETA producer. Complete phage genome sequences were determined, and a comparative analysis of five designed genomic regions revealed major variances between them. They differed in the genome size, number of open reading frames, genome architecture, and virion protein patterns. Their high mutual sequence similarity was detected only in the terminal regions of the genome. When compared with the so far described eta phage genomes, noticeable differences were found. Thus, both phages represent two new lineages of as yet not characterized bacteriophages of the Siphoviridae family having impact on pathogenicity of impetigo strains of S. aureus.
ER  -

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Hana KONEČNÁ, Roman PANTŮČEK, Ivan RYCHLÍK, Zbyněk ZDRÁHAL, Petr PETRÁŠ and Jiří DOŠKAŘ. Complete genome analysis of two new bacteriophages isolated from impetigo strains of Staphylococcus aureus. \textit{Virus Genes}. Springer US, 2015, vol.~51, No~1, p.~122-131. ISSN~0920-8569. Available from: https://dx.doi.org/10.1007/s11262-015-1223-8.

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