VIDLÁKOVÁ, Pavlína, Hana PIVOŇKOVÁ, Iva KEJNOVSKÁ, Libuše TRNKOVÁ, Michaela VORLÍČKOVÁ, Miroslav FOJTA a Luděk HAVRAN. G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques. Analytical and Bioanalytical chemistry. HEIDELBERG: SPRINGER HEIDELBERG, 2015, roč. 407, č. 19, s. 5817-5826. ISSN 1618-2642. Dostupné z: https://dx.doi.org/10.1007/s00216-015-8768-1. |
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@article{1308668, author = {Vidláková, Pavlína and Pivoňková, Hana and Kejnovská, Iva and Trnková, Libuše and Vorlíčková, Michaela and Fojta, Miroslav and Havran, Luděk}, article_location = {HEIDELBERG}, article_number = {19}, doi = {http://dx.doi.org/10.1007/s00216-015-8768-1}, keywords = {Oligonucleotides; Electrochemical methods; G-quadruplex; Hanging mercury drop electrode}, language = {eng}, issn = {1618-2642}, journal = {Analytical and Bioanalytical chemistry}, title = {G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques}, url = {http://download.springer.com/static/pdf/375/art%253A10.1007%252Fs00216-015-8768-1.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00216-015-8768-1&token2=exp=1439882775~acl=%2Fstatic%2Fpdf%2F375%2Fart%25253A10.1007%25252Fs00216-015-87}, volume = {407}, year = {2015} }
TY - JOUR ID - 1308668 AU - Vidláková, Pavlína - Pivoňková, Hana - Kejnovská, Iva - Trnková, Libuše - Vorlíčková, Michaela - Fojta, Miroslav - Havran, Luděk PY - 2015 TI - G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques JF - Analytical and Bioanalytical chemistry VL - 407 IS - 19 SP - 5817-5826 EP - 5817-5826 PB - SPRINGER HEIDELBERG SN - 16182642 KW - Oligonucleotides KW - Electrochemical methods KW - G-quadruplex KW - Hanging mercury drop electrode UR - http://download.springer.com/static/pdf/375/art%253A10.1007%252Fs00216-015-8768-1.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00216-015-8768-1&token2=exp=1439882775~acl=%2Fstatic%2Fpdf%2F375%2Fart%25253A10.1007%25252Fs00216-015-87 L2 - http://download.springer.com/static/pdf/375/art%253A10.1007%252Fs00216-015-8768-1.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00216-015-8768-1&token2=exp=1439882775~acl=%2Fstatic%2Fpdf%2F375%2Fart%25253A10.1007%25252Fs00216-015-87 N2 - Electrochemical methods, particularly when applied in connection with mercury-containing electrodes, are excellent tools for studying nucleic acids structure and monitoring structural transitions. We studied the effect of the length of the central (dG) (n) stretch (varying from 0 to 15 guanine residues) in 15-mer oligodeoxynucleotides (ODN, G0 to G15) on their electrochemical and interfacial behavior at mercury and carbon electrodes. The intensity of guanine oxidation signal at the carbon electrode (peak G(ox)) was observed to increase continuously with number of guanines between 0 and 15, with only a slight positive shift for ODNs with seven or more guanines in the central segment. Very different effects were observed when the peak G(HMDE) was measured at the mercury electrode. Intensity of the latter signal increased with number of guanines up to G5, and decreased sharply with further elongation of the (dG) (n) stretch. CD spectroscopy and electrophoresis experiments revealed formation of parallel intermolecular quadruplex structures for ODNs containing five or more G residues. Further measurements made by cyclic and alternating-current voltammetry revealed a strong influence of the ODN structure on their behavior at electrically charged surfaces. ER -
VIDLÁKOVÁ, Pavlína, Hana PIVOŇKOVÁ, Iva KEJNOVSKÁ, Libuše TRNKOVÁ, Michaela VORLÍČKOVÁ, Miroslav FOJTA a Luděk HAVRAN. G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2015, roč.~407, č.~19, s.~5817-5826. ISSN~1618-2642. Dostupné z: https://dx.doi.org/10.1007/s00216-015-8768-1.
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