Proteome-wide dataset generated by iTRAQ-3DLCMS/MS technique
for studying the role of FerB protein in oxidative stress in
Paracoccus denitrificans

J 2015

Proteome-wide dataset generated by iTRAQ-3DLCMS/MS technique for studying the role of FerB protein in oxidative stress in Paracoccus denitrificans

PERNIKÁŘOVÁ, Vendula, Vojtěch SEDLÁČEK, David POTĚŠIL, Iva PROCHÁZKOVÁ, Zbyněk ZDRÁHAL et. al.

Basic information

Original name

Proteome-wide dataset generated by iTRAQ-3DLCMS/MS technique for studying the role of FerB protein in oxidative stress in Paracoccus denitrificans

Authors

PERNIKÁŘOVÁ, Vendula (203 Czech Republic, belonging to the institution), Vojtěch SEDLÁČEK (203 Czech Republic, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution), Iva PROCHÁZKOVÁ (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Pavel BOUCHAL (203 Czech Republic, belonging to the institution) and Igor KUČERA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Data in Brief, Amsterdam, Elsevier, 2015, 2352-3409

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/15:00080961

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.dib.2015.06.015

UT WoS

000453157400072

Keywords in English

Paracoccus denitrificans; 3DLC proteomics; FerB; iTRAQ; Microbial proteomics

Abstract

V originále

3DLC protein- and peptide fractionation technique combined with iTRAQ-peptide labeling and Orbitrap mass spectrometry was employed to quantitate Paracoccus dentirificans total proteome with maximal coverage. This resulted in identification of 24948 peptides representing 2627 proteins (FDR<0.01) in P. dentirificans wild type and ferB mutant strains grown in the presence or absence of methyl viologen as an oxidative stressor. The data were generated for assessment of FerB protein role in oxidative stress as published by V. Pernikářová et al., Proteomic responses to a methyl viologeninduced oxidative stress in the wild type and FerB mutant strains of Paracoccus denitrificans, J Proteomics 2015;125:68-75. Dataset is supplied in the article.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GAP503/12/0369, research and development project

Name: Nové flavin-dependentní enzymy Paracoccus denitrificans: reakční mechanismy, metabolické funkce a úloha v buněčném oxidačním stresu

Investor: Czech Science Foundation

GA14-19250S, research and development project

Name: Nový panel proteinů korelujících se stavem lymfatických uzlin u low-grade nádorů prsu: Klinická verifikace a úloha v invazivitě nádorových buněk

Investor: Czech Science Foundation

Citovat

PERNIKÁŘOVÁ, Vendula, Vojtěch SEDLÁČEK, David POTĚŠIL, Iva PROCHÁZKOVÁ, Zbyněk ZDRÁHAL, Pavel BOUCHAL and Igor KUČERA. Proteome-wide dataset generated by iTRAQ-3DLCMS/MS technique for studying the role of FerB protein in oxidative stress in Paracoccus denitrificans. Data in Brief. Amsterdam: Elsevier, 2015, vol. 4, September, p. 390-394. ISSN 2352-3409. Available from: https://dx.doi.org/10.1016/j.dib.2015.06.015.

@article{1308777,
   author = {Pernikářová, Vendula and Sedláček, Vojtěch and Potěšil, David and Procházková, Iva and Zdráhal, Zbyněk and Bouchal, Pavel and Kučera, Igor},
   article_location = {Amsterdam},
   article_number = {September},
   doi = {http://dx.doi.org/10.1016/j.dib.2015.06.015},
   keywords = {Paracoccus denitrificans; 3DLC proteomics; FerB; iTRAQ; Microbial proteomics},
   language = {eng},
   issn = {2352-3409},
   journal = {Data in Brief},
   title = {Proteome-wide dataset generated by iTRAQ-3DLCMS/MS technique for studying the role of FerB protein in oxidative stress in Paracoccus denitrificans},
   volume = {4},
   year = {2015}
}

TY  - JOUR
ID  - 1308777
AU  - Pernikářová, Vendula - Sedláček, Vojtěch - Potěšil, David - Procházková, Iva - Zdráhal, Zbyněk - Bouchal, Pavel - Kučera, Igor
PY  - 2015
TI  - Proteome-wide dataset generated by iTRAQ-3DLCMS/MS technique for studying the role of FerB protein in oxidative stress in Paracoccus denitrificans
JF  - Data in Brief
VL  - 4
IS  - September
SP  - 390-394
EP  - 390-394
PB  - Elsevier
SN  - 23523409
KW  - Paracoccus denitrificans
KW  - 3DLC proteomics
KW  - FerB
KW  - iTRAQ
KW  - Microbial proteomics
N2  - 3DLC protein- and peptide fractionation technique combined with iTRAQ-peptide labeling and Orbitrap mass spectrometry was employed to quantitate Paracoccus dentirificans total proteome with maximal coverage. This resulted in identification of 24948 peptides representing 2627 proteins (FDR<0.01) in P. dentirificans wild type and ferB mutant strains grown in the presence or absence of methyl viologen as an oxidative stressor. The data were generated for assessment of FerB protein role in oxidative stress as published by V. Pernikářová et al., Proteomic responses to a methyl viologeninduced oxidative stress in the wild type and FerB mutant strains of Paracoccus denitrificans, J Proteomics 2015;125:68-75. Dataset is supplied in the article.
ER  -

PERNIKÁŘOVÁ, Vendula, Vojtěch SEDLÁČEK, David POTĚŠIL, Iva PROCHÁZKOVÁ, Zbyněk ZDRÁHAL, Pavel BOUCHAL and Igor KUČERA. Proteome-wide dataset generated by iTRAQ-3DLCMS/MS technique for studying the role of FerB protein in oxidative stress in Paracoccus denitrificans. \textit{Data in Brief}. Amsterdam: Elsevier, 2015, vol.~4, September, p.~390-394. ISSN~2352-3409. Available from: https://dx.doi.org/10.1016/j.dib.2015.06.015.

Detailed Information on Publication Record