The article focuses on evaluation of stress conditions in scenario training during an self-defence course. According to Yerkes-Dodson law optimal task performance occurs at an intermediate level of arousal, with relatively poorer performance at both lower and higher arousal levels, leading to an inverted U relation between arousal and performance. Scenario training (ST) is one of crucial parts of self-defence teaching. The aim of the study was to evaluate level of acute stress, which was induce to probands participating in self-defence ST. ST could be considered both training and evaluation method. There are more manners how to use ST and implement it into self-defence training. Using ST as a training method stress should be set on the intermediate level to arouse optimal performance. When using ST as an evaluation method stress conditions should be set on level crossing the intermadiate level to provoke errors, which are basis for the ex-post evaluation and reflexion. The essential factor of ST is presence of psychological stress and dynamic environment. Methods The sample consisted of university students of sport study programme. Total number of probands n=41, males n=18, females n=23. Non-standardised questionaire was used for the analysis purpose. The tool consisted of 19 closed questions with scale 0 – 6 and 3 open questions. Open questions were analysed with the scientific software Atlast.ti. Results Subjects admited they felt fear (M=2,4) and threatened (M=3,1). Also physiological symptoms of stress described in literature were present: trembling with fear (M=2,0), sweat (M=2,1), palpitations (M=3,1). Probands were during ST in certain stress conditins. However, there are big differences between individuals, average values are rather lower. Better setting of stress conditions aproaching or crossing the intermediate level of arousal according to Yerkes-Dodson law is recommended.