| VRBSKÝ, Jan, Tamás TEREH, Sergiy KYRYLENKO, Petr DVOŘÁK and Lumír KREJČÍ. MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor. Plos one. San Francisco: Public Library of Science, 2015, vol. 10, No 6, p. "e0127739", 16 pp. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0127739. |
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@article{1309636,
author = {Vrbský, Jan and Tereh, Tamás and Kyrylenko, Sergiy and Dvořák, Petr and Krejčí, Lumír},
article_location = {San Francisco},
article_number = {6},
doi = {http://dx.doi.org/10.1371/journal.pone.0127739},
keywords = {PLURIPOTENT STEM-CELLS; HUMAN SOMATIC-CELLS; SMALL-MOLECULE COMPOUNDS; PRIMORDIAL GERM-CELLS; SELF-RENEWAL; NEURONAL DIFFERENTIATION; PROGENITOR CELLS; MOUSE; INDUCTION; NANOG},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor},
volume = {10},
year = {2015}
}
TY - JOUR
ID - 1309636
AU - Vrbský, Jan - Tereh, Tamás - Kyrylenko, Sergiy - Dvořák, Petr - Krejčí, Lumír
PY - 2015
TI - MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor
JF - Plos one
VL - 10
IS - 6
SP - "e0127739"
EP - "e0127739"
PB - Public Library of Science
SN - 19326203
KW - PLURIPOTENT STEM-CELLS
KW - HUMAN SOMATIC-CELLS
KW - SMALL-MOLECULE COMPOUNDS
KW - PRIMORDIAL GERM-CELLS
KW - SELF-RENEWAL
KW - NEURONAL DIFFERENTIATION
KW - PROGENITOR CELLS
KW - MOUSE
KW - INDUCTION
KW - NANOG
N2 - The possibility of replacing the originally discovered and widely used DNA reprogramming transcription factors is stimulating enormous effort to identify more effective compounds that would not alter the genetic information. Here, we describe the generation of induced pluripotent stem cells (iPSc) from head-derived primary culture of mouse embryonic cells using small chemical inhibitors of the MEK and TGF-beta pathways without delivery of exogenous transcription factors. These iPSc express standard pluripotency markers and retain their potential to differentiate into cells of all germ layers. Our data indicate that head-derived embryonic neural cells might have the reprogramming potential while neither the same primary cells cultivated over five passages in vitro nor a cell population derived from adult brain possesses this capacity. Our results reveal the potential for small molecules to functionally replace routinely used transcription factors and lift the veil on molecular regulation controlling pluripotency. The conditions described here could provide a platform upon which other genome non integrative and safer reprogramming processes could be developed. This work also shows novel potential for developing embryonic neural cells.
ER -
VRBSKÝ, Jan, Tamás TEREH, Sergiy KYRYLENKO, Petr DVOŘÁK and Lumír KREJČÍ. MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor. \textit{Plos one}. San Francisco: Public Library of Science, 2015, vol.~10, No~6, p.~''e0127739'', 16 pp. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0127739.
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