| SAMEK, Ota, Silvie BERNATOVÁ, Jan JEŽEK, Martin ŠILER, Mojmir ŠERÝ, Vladislav KRZYŽÁNEK, Kamila HRUBANOVÁ, Pavel ZEMÁNEK, Veronika HOLÁ a Filip RŮŽIČKA. Identification of individual biofilm-forming bacterial cells using Raman tweezers. Journal of Biomedical Optics. Bellingham: SPIE, roč. 20, č. 5, s. "051038-1"-"051038-6", 6 s. ISSN 1083-3668. doi:10.1117/1.JBO.20.5.051038. 2015. |
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@article{1309673,
author = {Samek, Ota and Bernatová, Silvie and Ježek, Jan and Šiler, Martin and Šerý, Mojmir and Krzyžánek, Vladislav and Hrubanová, Kamila and Zemánek, Pavel and Holá, Veronika and Růžička, Filip},
article_location = {Bellingham},
article_number = {5},
doi = {http://dx.doi.org/10.1117/1.JBO.20.5.051038},
keywords = {Raman tweezers; Staphylococcus epidermidis; biofilm},
language = {eng},
issn = {1083-3668},
journal = {Journal of Biomedical Optics},
title = {Identification of individual biofilm-forming bacterial cells using Raman tweezers},
volume = {20},
year = {2015}
}
TY - JOUR
ID - 1309673
AU - Samek, Ota - Bernatová, Silvie - Ježek, Jan - Šiler, Martin - Šerý, Mojmir - Krzyžánek, Vladislav - Hrubanová, Kamila - Zemánek, Pavel - Holá, Veronika - Růžička, Filip
PY - 2015
TI - Identification of individual biofilm-forming bacterial cells using Raman tweezers
JF - Journal of Biomedical Optics
VL - 20
IS - 5
SP - "051038-1"-"051038-6"
EP - "051038-1"-"051038-6"
PB - SPIE
SN - 10833668
KW - Raman tweezers
KW - Staphylococcus epidermidis
KW - biofilm
N2 - A method for in vitro identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral "Raman fingerprints" obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilmnegative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of Staphylococcus epidermidis. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment.
ER -
SAMEK, Ota, Silvie BERNATOVÁ, Jan JEŽEK, Martin ŠILER, Mojmir ŠERÝ, Vladislav KRZYŽÁNEK, Kamila HRUBANOVÁ, Pavel ZEMÁNEK, Veronika HOLÁ a Filip RŮŽIČKA. Identification of individual biofilm-forming bacterial cells using Raman tweezers. \textit{Journal of Biomedical Optics}. Bellingham: SPIE, roč.~20, č.~5, s.~''051038-1''-''051038-6'', 6 s. ISSN~1083-3668. doi:10.1117/1.JBO.20.5.051038. 2015.
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