Identification of individual biofilm-forming bacterial cells
using Raman tweezers

J 2015

Identification of individual biofilm-forming bacterial cells using Raman tweezers

SAMEK, Ota, Silvie BERNATOVÁ, Jan JEŽEK, Martin ŠILER, Mojmir ŠERÝ et. al.

Basic information

Original name

Identification of individual biofilm-forming bacterial cells using Raman tweezers

Authors

SAMEK, Ota (203 Czech Republic), Silvie BERNATOVÁ (203 Czech Republic), Jan JEŽEK (203 Czech Republic), Martin ŠILER (203 Czech Republic), Mojmir ŠERÝ (203 Czech Republic), Vladislav KRZYŽÁNEK (203 Czech Republic), Kamila HRUBANOVÁ (203 Czech Republic), Pavel ZEMÁNEK (203 Czech Republic), Veronika HOLÁ (203 Czech Republic, belonging to the institution) and Filip RŮŽIČKA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Biomedical Optics, Bellingham, SPIE, 2015, 1083-3668

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.556

RIV identification code

RIV/00216224:14110/15:00080989

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1117/1.JBO.20.5.051038

UT WoS

000356241900043

Keywords in English

Raman tweezers; Staphylococcus epidermidis; biofilm

Abstract

V originále

A method for in vitro identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral "Raman fingerprints" obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilmnegative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of Staphylococcus epidermidis. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment.

Links

GAP205/11/1687, research and development project

Name: Neinvazní bezkontaktní metody identifikace a charakterizace živých mikroorganismů prostřednictvím optické spektroskopie a mikromanipulace

Investor: Czech Science Foundation

NS9678, research and development project

Name: Biofilm - významný faktor virulence u infekcí močových katétrů; detekce a možnosti ovlivnění

Investor: Ministry of Health of the CR

Citovat

SAMEK, Ota, Silvie BERNATOVÁ, Jan JEŽEK, Martin ŠILER, Mojmir ŠERÝ, Vladislav KRZYŽÁNEK, Kamila HRUBANOVÁ, Pavel ZEMÁNEK, Veronika HOLÁ and Filip RŮŽIČKA. Identification of individual biofilm-forming bacterial cells using Raman tweezers. Journal of Biomedical Optics. Bellingham: SPIE, 2015, vol. 20, No 5, p. "051038-1"-"051038-6", 6 pp. ISSN 1083-3668. Available from: https://dx.doi.org/10.1117/1.JBO.20.5.051038.

@article{1309673,
   author = {Samek, Ota and Bernatová, Silvie and Ježek, Jan and Šiler, Martin and Šerý, Mojmir and Krzyžánek, Vladislav and Hrubanová, Kamila and Zemánek, Pavel and Holá, Veronika and Růžička, Filip},
   article_location = {Bellingham},
   article_number = {5},
   doi = {http://dx.doi.org/10.1117/1.JBO.20.5.051038},
   keywords = {Raman tweezers; Staphylococcus epidermidis; biofilm},
   language = {eng},
   issn = {1083-3668},
   journal = {Journal of Biomedical Optics},
   title = {Identification of individual biofilm-forming bacterial cells using Raman tweezers},
   volume = {20},
   year = {2015}
}

TY  - JOUR
ID  - 1309673
AU  - Samek, Ota - Bernatová, Silvie - Ježek, Jan - Šiler, Martin - Šerý, Mojmir - Krzyžánek, Vladislav - Hrubanová, Kamila - Zemánek, Pavel - Holá, Veronika - Růžička, Filip
PY  - 2015
TI  - Identification of individual biofilm-forming bacterial cells using Raman tweezers
JF  - Journal of Biomedical Optics
VL  - 20
IS  - 5
SP  - "051038-1"-"051038-6"
EP  - "051038-1"-"051038-6"
PB  - SPIE
SN  - 10833668
KW  - Raman tweezers
KW  - Staphylococcus epidermidis
KW  - biofilm
N2  - A method for in vitro identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral "Raman fingerprints" obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilmnegative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of Staphylococcus epidermidis. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment.
ER  -

SAMEK, Ota, Silvie BERNATOVÁ, Jan JEŽEK, Martin ŠILER, Mojmir ŠERÝ, Vladislav KRZYŽÁNEK, Kamila HRUBANOVÁ, Pavel ZEMÁNEK, Veronika HOLÁ and Filip RŮŽIČKA. Identification of individual biofilm-forming bacterial cells using Raman tweezers. \textit{Journal of Biomedical Optics}. Bellingham: SPIE, 2015, vol.~20, No~5, p.~''051038-1''-''051038-6'', 6 pp. ISSN~1083-3668. Available from: https://dx.doi.org/10.1117/1.JBO.20.5.051038.

Detailed Information on Publication Record