BÁRTOVÁ, Eva, Tereza MACHAČOVÁ, Kamil SEDLÁK, Marie BUDÍKOVÁ, Ugo MARIANI and Vincenzo VENEZIANO. Seroprevalence of antibodies of Neospora spp. and Toxoplasma gondii in horses from southern Italy. Folia Parasitologica. Praha: AV ČR, Institute of Parasitology, Biology Centrum, 2015, vol. 62, July, p. 1-4. ISSN 0015-5683. Available from: https://dx.doi.org/10.14411/fp.2015.043.
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Basic information
Original name Seroprevalence of antibodies of Neospora spp. and Toxoplasma gondii in horses from southern Italy
Name in Czech Séroprevalence protilátek proti Neospora spp. a Toxoplasma gondii u koní v jižní Itálii
Authors BÁRTOVÁ, Eva (203 Czech Republic, guarantor), Tereza MACHAČOVÁ (203 Czech Republic), Kamil SEDLÁK (203 Czech Republic), Marie BUDÍKOVÁ (203 Czech Republic, belonging to the institution), Ugo MARIANI (380 Italy) and Vincenzo VENEZIANO (380 Italy).
Edition Folia Parasitologica, Praha, AV ČR, Institute of Parasitology, Biology Centrum, 2015, 0015-5683.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30300 3.3 Health sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 1.271
RIV identification code RIV/00216224:14310/15:00083771
Organization unit Faculty of Science
Doi http://dx.doi.org/10.14411/fp.2015.043
UT WoS 000364837200001
Keywords (in Czech) sérologický test; Equus caballus; rizikový faktor; toxoplasmoza; neosporoza
Keywords in English serological test; Equus caballus; risk factor; toxoplasmosis; neosporosis
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Nicole Zrilić, učo 240776. Changed: 25/5/2017 16:21.
Abstract
The consumption of horse meat has been epidemiologically linked to clinical toxoplasmosis in humans and neosporosis that may cause clinical illness in horses. Here we determined seroprevalence of antibodies against Toxoplasma gondii Nicolle et Manceaux, 1908 and species of Neospora Dubey, Carpenter, Speer, Topper et Uggla, 1988 in horses from Italy. Blood samples were collected from 643 apparently healthy horses from 60 farms of 51 municipalities in southern Italy. The presence of antibodies against T. gondii and Neospora spp. were detected by indirect fluorescence antibody test (IFAT); a titre at least 50 was considered positive. The same sera were also tested for antibodies against Neospora spp. by a competitive-inhibition enzyme-linked immunosorbent assay (cELISA); samples with at least 30% inhibition were considered positive. Antibodies against T. gondii and Neospora spp. were detected in 19 (3.0%) and 15 (2.3%) horses by IFAT, respectively, without statistical difference between gender, age and breeds (p-value greater than 0.05). Antibodies against species of Neospora were detected in 70 (10.9%) horses by cELISA with statistical difference in gender (6.0–18.5%, p-value less than 0.05) and breeds (0–19.4%, p-value less than 0.05). Although T. gondii infection rates were low, the risk of human infection should not be dismissed, particularly in Italy where consumption of raw or undercooked horse meat has a long tradition.
Abstract (in Czech)
Konzumace koňského masa byla epidemiologicky spojena s klinickou toxoplasmózou u lidí a neosporózou, které mohou způsobit klinické onemocnění u koní. Zde jsme určovali séroprevalence protilátek proti Toxoplasma gondii (Nicolle et Manceaux, 1908) a Neospora spp. (Dubey, Carpenter, Speer, Topper et Uggla, 1988) u koní z Itálie. Krevní vzorky byly odebrány od 643 zjevně zdravých koní z 60 farem v 51 obcích v jižní Itálii. Přítomnost protilátek proti T. gondii a Neospora spp. byly detekovány testem IFAT; titr aspoň 50 byl považován za pozitivní. Stejné sérum bylo také testováno na protilátky proti Neospora spp. pomocí cELISA; vzorky s aspoň 30% inhibici byly považovány za pozitivní. Protilátky proti T. gondii a Neospora spp. byly detekovány u 19 (3,0%) a 15 (2,3%) koní podle IFAT, v daném pořadí, a to bez statisticky významného vlivu pohlaví, věku a plemene (p-hodnota větší než 0,05). Protilátky proti Neospora byly zjištěny u 70 (10,9%) koní podle cELISA. Zde se prokázal statisticky významný rozdíl v pohlaví (6.0-18.5%, p-hodnota menší než 0,05) a plemenu (0-19.4%, p-hodnota menší než 0,05). Ačkoli výskyt T. gondii byl nízký, riziko nákazy člověka by nemělo být podceňováno, zejména v Itálii, kde konzumace syrového nebo nedostatečně tepelně upraveného koňského masa má dlouhou tradici.
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