Paramagnetic Nanoparticles as a Platform for FRET-Based
Sarcosine Picomolar Detection

J 2015

Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection

HEGER, Zbynek, Natalia CERNEI, Sona KRIZKOVA, Michal MASAŘÍK, Pavel KOPEL et. al.

Basic information

Original name

Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection

Authors

HEGER, Zbynek (203 Czech Republic), Natalia CERNEI (203 Czech Republic), Sona KRIZKOVA (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution), Pavel KOPEL (203 Czech Republic), Petr HODEK (203 Czech Republic), Ondrej ZITKA (203 Czech Republic), Vojtech ADAM (203 Czech Republic) and Rene KIZEK (203 Czech Republic)

Edition

Scientific Reports, London, Nature Publishing Group, 2015, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.228

RIV identification code

RIV/00216224:14110/15:00083933

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/srep08868

UT WoS

000350549200018

Keywords in English

Prostate-cancer progression; Urine samples; Diagnostics; Carcinoma

Abstract

V originále

Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Forster resonance energy transfer (FRET). The FRET biosensor employs anti-sarcosine antibodies immobilized on paramagnetic nanoparticles surface for specific antigen binding. Successful binding of sarcosine leads to assembly of a sandwich construct composed of anti-sarcosine antibodies keeping the Forster distance (Ro) of FRET pair in required proximity. The detection is based on spectral overlap between gold-functionalized green fluorescent protein and antibodies@quantum dots bioconjugate (lambda(ex) 400 nm). The saturation curve of sarcosine based on FRET efficiency (F-604/F-510 ratio) was tested within linear dynamic range from 5 to 50 nM with detection limit down to 50 pM. Assembled biosensor was then successfully employed for sarcosine quantification in prostatic cell lines (PC3, 22Rv1, PNT1A), and urinary samples of prostate adenocarcinoma patients.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

HEGER, Zbynek, Natalia CERNEI, Sona KRIZKOVA, Michal MASAŘÍK, Pavel KOPEL, Petr HODEK, Ondrej ZITKA, Vojtech ADAM and Rene KIZEK. Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection. Scientific Reports. London: Nature Publishing Group, 2015, vol. 5, "8868", p. 1-7. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/srep08868.

@article{1311819,
   author = {Heger, Zbynek and Cernei, Natalia and Krizkova, Sona and Masařík, Michal and Kopel, Pavel and Hodek, Petr and Zitka, Ondrej and Adam, Vojtech and Kizek, Rene},
   article_location = {London},
   article_number = {"8868"},
   doi = {http://dx.doi.org/10.1038/srep08868},
   keywords = {Prostate-cancer progression; Urine samples; Diagnostics; Carcinoma},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection},
   volume = {5},
   year = {2015}
}

TY  - JOUR
ID  - 1311819
AU  - Heger, Zbynek - Cernei, Natalia - Krizkova, Sona - Masařík, Michal - Kopel, Pavel - Hodek, Petr - Zitka, Ondrej - Adam, Vojtech - Kizek, Rene
PY  - 2015
TI  - Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection
JF  - Scientific Reports
VL  - 5
IS  - "8868"
SP  - 1-7
EP  - 1-7
PB  - Nature Publishing Group
SN  - 20452322
KW  - Prostate-cancer progression
KW  - Urine samples
KW  - Diagnostics
KW  - Carcinoma
N2  - Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Forster resonance energy transfer (FRET). The FRET biosensor employs anti-sarcosine antibodies immobilized on paramagnetic nanoparticles surface for specific antigen binding. Successful binding of sarcosine leads to assembly of a sandwich construct composed of anti-sarcosine antibodies keeping the Forster distance (Ro) of FRET pair in required proximity. The detection is based on spectral overlap between gold-functionalized green fluorescent protein and antibodies@quantum dots bioconjugate (lambda(ex) 400 nm). The saturation curve of sarcosine based on FRET efficiency (F-604/F-510 ratio) was tested within linear dynamic range from 5 to 50 nM with detection limit down to 50 pM. Assembled biosensor was then successfully employed for sarcosine quantification in prostatic cell lines (PC3, 22Rv1, PNT1A), and urinary samples of prostate adenocarcinoma patients.
ER  -

HEGER, Zbynek, Natalia CERNEI, Sona KRIZKOVA, Michal MASAŘÍK, Pavel KOPEL, Petr HODEK, Ondrej ZITKA, Vojtech ADAM and Rene KIZEK. Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection. \textit{Scientific Reports}. London: Nature Publishing Group, 2015, vol.~5, ''8868'', p.~1-7. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/srep08868.

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