17 beta-estradiol-containing liposomes as a novel delivery
system for the antisense therapy of ER-positive breast cancer:
An in vitro study on the MCF-7 cell line

J 2015

17 beta-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line

HEGER, Zbynek, Jaromír GUMULEC, Natalia CERNEI, Katerina TMEJOVA, Pavel KOPEL et. al.

Basic information

Original name

17 beta-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line

Authors

HEGER, Zbynek (203 Czech Republic), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Natalia CERNEI (498 Republic of Moldova), Katerina TMEJOVA (203 Czech Republic), Pavel KOPEL (203 Czech Republic), Jan BALVAN (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution), Ondrej ZITKA (203 Czech Republic), Miroslava BEKLOVA (203 Czech Republic), Vojtech ADAM (203 Czech Republic) and Rene KIZEK (203 Czech Republic)

Edition

Oncology Reports, Athens, Spandidos Publications, 2015, 1021-335X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Greece

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.486

RIV identification code

RIV/00216224:14110/15:00083937

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3892/or.2014.3627

UT WoS

000348338500054

Keywords in English

antisense therapy; delivery; glutathione; liposome; malondialdehyde; metallothionein

Abstract

V originále

The present study suggests and describes the application of a delivery system for antisense oligonucleotides against mRNA encoding estrogen receptor proteins alpha and beta. The delivery system is composed of a cationic liposome envelope containing 17 beta-estradiol (E-2) in its structure. Cationic liposomes protect cargo against the extracellular matrix, and E-2 can increase its shuttling efficiency into cells. Using MCF-7 cells derived from estrogen receptor-positive ductal carcinoma, treatment with liposomes against ER alpha was found to decrease MCF-7 proliferation, and importantly the application of both the antisense against ER alpha and beta exhibited an antiproliferative effect expressed as cell viability. Using qRT-PCR, it was shown that MTIA,NF-kappa B1 and K-ras genes, but not TFF1, were downregulated using E-2-based liposomes (evaluated at P=0.05). Further indicators of oxidative stress were employed to assess the effect on treatment efficiency. Glutathione (GSH/GSSG redox ratio), metallothionein (MT) and malondialdehyde (MDA) confirmed a positive effect of antisense therapy resulting in their decreased levels in the MCF-7 cells. Based on these data, we suggest that E2-based liposomes offer sufficient transfer efficiency and moreover, due to the effect on NF-kappa BI, MT and GSH, tumor cells can be chemosensitized to increase treatment effectiveness.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

HEGER, Zbynek, Jaromír GUMULEC, Natalia CERNEI, Katerina TMEJOVA, Pavel KOPEL, Jan BALVAN, Michal MASAŘÍK, Ondrej ZITKA, Miroslava BEKLOVA, Vojtech ADAM and Rene KIZEK. 17 beta-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line. Oncology Reports. Athens: Spandidos Publications, 2015, vol. 33, No 2, p. 921-929. ISSN 1021-335X. Available from: https://dx.doi.org/10.3892/or.2014.3627.

@article{1311838,
   author = {Heger, Zbynek and Gumulec, Jaromír and Cernei, Natalia and Tmejova, Katerina and Kopel, Pavel and Balvan, Jan and Masařík, Michal and Zitka, Ondrej and Beklova, Miroslava and Adam, Vojtech and Kizek, Rene},
   article_location = {Athens},
   article_number = {2},
   doi = {http://dx.doi.org/10.3892/or.2014.3627},
   keywords = {antisense therapy; delivery; glutathione; liposome; malondialdehyde; metallothionein},
   language = {eng},
   issn = {1021-335X},
   journal = {Oncology Reports},
   title = {17 beta-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line},
   volume = {33},
   year = {2015}
}

TY  - JOUR
ID  - 1311838
AU  - Heger, Zbynek - Gumulec, Jaromír - Cernei, Natalia - Tmejova, Katerina - Kopel, Pavel - Balvan, Jan - Masařík, Michal - Zitka, Ondrej - Beklova, Miroslava - Adam, Vojtech - Kizek, Rene
PY  - 2015
TI  - 17 beta-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line
JF  - Oncology Reports
VL  - 33
IS  - 2
SP  - 921-929
EP  - 921-929
PB  - Spandidos Publications
SN  - 1021335X
KW  - antisense therapy
KW  - delivery
KW  - glutathione
KW  - liposome
KW  - malondialdehyde
KW  - metallothionein
N2  - The present study suggests and describes the application of a delivery system for antisense oligonucleotides against mRNA encoding estrogen receptor proteins alpha and beta. The delivery system is composed of a cationic liposome envelope containing 17 beta-estradiol (E-2) in its structure. Cationic liposomes protect cargo against the extracellular matrix, and E-2 can increase its shuttling efficiency into cells. Using MCF-7 cells derived from estrogen receptor-positive ductal carcinoma, treatment with liposomes against ER alpha was found to decrease MCF-7 proliferation, and importantly the application of both the antisense against ER alpha and beta exhibited an antiproliferative effect expressed as cell viability. Using qRT-PCR, it was shown that MTIA,NF-kappa B1 and K-ras genes, but not TFF1, were downregulated using E-2-based liposomes (evaluated at P=0.05). Further indicators of oxidative stress were employed to assess the effect on treatment efficiency. Glutathione (GSH/GSSG redox ratio), metallothionein (MT) and malondialdehyde (MDA) confirmed a positive effect of antisense therapy resulting in their decreased levels in the MCF-7 cells. Based on these data, we suggest that E2-based liposomes offer sufficient transfer efficiency and moreover, due to the effect on NF-kappa BI, MT and GSH, tumor cells can be chemosensitized to increase treatment effectiveness.
ER  -

HEGER, Zbynek, Jaromír GUMULEC, Natalia CERNEI, Katerina TMEJOVA, Pavel KOPEL, Jan BALVAN, Michal MASAŘÍK, Ondrej ZITKA, Miroslava BEKLOVA, Vojtech ADAM and Rene KIZEK. 17 beta-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line. \textit{Oncology Reports}. Athens: Spandidos Publications, 2015, vol.~33, No~2, p.~921-929. ISSN~1021-335X. Available from: https://dx.doi.org/10.3892/or.2014.3627.

Detailed Information on Publication Record