Polymorphism in miR-31 and miR-584 binding site in the angiotensinogen gene differentially influences body fat distribution in both sexes

MÁCHAL, Jan, Jan NOVÁK, Renata HÉŽOVÁ, Filip ZLÁMAL, Anna VAŠKŮ, Ondřej SLABÝ and Julie BIENERTOVÁ VAŠKŮ. Polymorphism in miR-31 and miR-584 binding site in the angiotensinogen gene differentially influences body fat distribution in both sexes. Genes and Nutrition. New York: Springer, 2015, vol. 10, No 5, p. 1-7. ISSN 1555-8932. doi:10.1007/s12263-015-0488-9.

Basic information

• Original name: Polymorphism in miR-31 and miR-584 binding site in the angiotensinogen gene differentially influences body fat distribution in both sexes
• Authors: MÁCHAL, Jan (203 Czech Republic, belonging to the institution), Jan NOVÁK (203 Czech Republic, belonging to the institution), Renata HÉŽOVÁ (203 Czech Republic, belonging to the institution), Filip ZLÁMAL (203 Czech Republic, belonging to the institution), Anna VAŠKŮ (203 Czech Republic, belonging to the institution), Ondřej SLABÝ (203 Czech Republic, belonging to the institution) and Julie BIENERTOVÁ VAŠKŮ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Genes and Nutrition, New York, Springer, 2015, 1555-8932.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.398
• RIV identification code: RIV/00216224:14110/15:00084163
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.1007/s12263-015-0488-9
• UT WoS: 000360820100011
• Keywords (in Czech): mikroRNA; 3-UTR polymorfismus; genetická variabilita; Rs7079; tuková tkáň
• Keywords in English: microRNA; 3-UTR polymorphism; Genetic variability; Rs7079; Adipose tissue
• Tags: EL OK, podil
• Tags: International impact, Reviewed

Abstract

Angiotensinogen (AGT), its active fragments and microRNA-31 (miR-31) play an important role in adipocyte differentiation. AGT contains a miR-31 polymorphic binding site. We hypothesize that the rs7079 polymorphism in the miR-31/584 binding site of the AGT gene could influence body fat distribution. A total of 751 subjects (195 men, 556 women) were enrolled in the study. The rs7079 genotypes were determined by qRT-PCR. Anthropometric measurements were taken on all subjects, who were subsequently divided into two groups: obese ([30 kg m-2 ) and non-obese (\30 kg m-2 ). Linear regression models were created to determine the contributions of sex, obesity status and rs7079 to all measured parameters. Adding the rs7079 genotype significantly contributed to the linear regression model for waist circumference (p = 0.013), hip circumference (p = 0.018) and supraspinal skin-fold thickness (p = 1 9 10-3 ). Differences between sexes and between the obese and nonobese groups were observed. Waist circumference was lower in men carrying the A allele (p = 0.022); hip circumference was higher only in obese women carrying the A allele (p = 0.015). While men carrying the A allele had lower supraspinal skin-fold thickness (p = 0.022), this parameter was found to be higher in A allele carrying women (p = 3 9 10-3 ). The higher total sum of skin-fold thickness in A allele carrying women was restricted to obese individuals (p = 0.028). The presence of the A allele was associated with both lower tricipital skin-fold thickness in non-obese women (p = 0.023) and a trend of higher thickness in non-obese men (p = 0.065). Significant associations of rs7079 in the AGT gene and body fat distribution were observed. The distribution followed opposing patterns in both sexes.