RUDÁ, Jana, Petra AMCHOVÁ, Tereza HAVLICKOVA, Pavel JERABEK, Zuzana BABINSKÁ, Petr KACER, Kamila SYSLOVA, Alexandra ŠULCOVÁ and Magdalena SUSTKOVA-FISEROVA. Reward related neurotransmitter changes in a model of depression: An in vivo microdialysis study. The World Journal of Biological Psychiatry. Abingdon: Taylor & Francis LTD, 2015, vol. 16, No 7, p. 521-535. ISSN 1562-2975. doi:10.3109/15622975.2015.1077991.
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Basic information
Original name Reward related neurotransmitter changes in a model of depression: An in vivo microdialysis study
Name in Czech Změny neurotransmiterů okruhu odměny v modelu deprese: in vivo mikrodialyzační studie
Authors RUDÁ, Jana (203 Czech Republic, guarantor, belonging to the institution), Petra AMCHOVÁ (203 Czech Republic, belonging to the institution), Tereza HAVLICKOVA (203 Czech Republic), Pavel JERABEK (203 Czech Republic), Zuzana BABINSKÁ (703 Slovakia, belonging to the institution), Petr KACER (203 Czech Republic), Kamila SYSLOVA (203 Czech Republic), Alexandra ŠULCOVÁ (203 Czech Republic, belonging to the institution) and Magdalena SUSTKOVA-FISEROVA (203 Czech Republic).
Edition The World Journal of Biological Psychiatry, Abingdon, Taylor & Francis LTD, 2015, 1562-2975.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30104 Pharmacology and pharmacy
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 4.159
RIV identification code RIV/00216224:14110/15:00084203
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.3109/15622975.2015.1077991
UT WoS 000369761900007
Keywords in English Depression; in vivo microdialysis; methamphetamine; olfactory bulbectomy; rats
Tags EL OK, podil
Tags International impact, Reviewed
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 8. 4. 2016 09:54.
Abstract
OBJECTIVES: The self-medication hypothesis assumes that symptoms related to potential monoaminergic deficits in depression may be relieved by drug abuse. The aim of this study was to elucidate the neurotransmitter changes in a rat model of depression by measuring their levels in the nucleus accumbens shell, which is typically involved in the drug of abuse acquisition mechanism. METHODS: Depression was modelled by the olfactory bulbectomy (OBX) in Wistar male rats. In vivo microdialysis was performed, starting from the baseline and following after a single methamphetamine injection and behaviour was monitored. The determination of neurotransmitters and their metabolites was performed by high-performance liquid chromatography combined with mass spectrometry. RESULTS: OBX animals had lower basal levels of dopamine and serotonin and their metabolites. However, GABA and glutamate levels were increased. The methamphetamine injection induced stronger dopamine and serotonin release in the OBX rats and lower release of glutamate in comparison with sham-operated rats; GABA levels did not differ significantly. CONCLUSIONS: This study provides an evidence of mesolimbic neurotransmitter changes in the rat model of depression which may elucidate mechanisms underlying intravenous self-administration studies in which OBX rats were demonstrated to have higher drug intake in comparison to intact controls.
Abstract (in Czech)
Ve studii byly sledovány změny hladin dopaminu a jeho metabolitů v nucleus accumbens shell metodou in vivo mikrodialýzy u OBX a slepě operovaných zvířat po akutní dávce metamfetaminu. U OBX zvířat bylo zaznamenáno snížení bazálních hladin dopaminu a jeho metabolitů. Dávka metamfetaminu (5 mg/kg) však vyvolala u OBX ve srovnání s kontrolou vyšší vyplavení dopaminu a zvýšení koncentrace jeho metabolitů. Získaná data potvrzují odlišnou reaktivitu zvířat s depresi podobným fenotypem na psychostimulancia.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
MUNI/A/0886/2013, interní kód MUName: Farmakodynamické, farmakokinetické a farmakogenetické přístupy v preklinické fázi výzkumu a vývoje léčiv (Acronym: FDN, FKK a FGEN studie)
Investor: Masaryk University, Category A
MUNI/A/1116/2014, interní kód MUName: Nové farmakologické mechanizmy v preklinickém i klinickém vývoji léčiv
Investor: Masaryk University, Category A
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