Detailed Information on Publication Record
2015
Reward related neurotransmitter changes in a model of depression: An in vivo microdialysis study
RUDÁ, Jana, Petra AMCHOVÁ, Tereza HAVLICKOVA, Pavel JERABEK, Zuzana BABINSKÁ et. al.Basic information
Original name
Reward related neurotransmitter changes in a model of depression: An in vivo microdialysis study
Name in Czech
Změny neurotransmiterů okruhu odměny v modelu deprese: in vivo mikrodialyzační studie
Authors
RUDÁ, Jana (203 Czech Republic, guarantor, belonging to the institution), Petra AMCHOVÁ (203 Czech Republic, belonging to the institution), Tereza HAVLICKOVA (203 Czech Republic), Pavel JERABEK (203 Czech Republic), Zuzana BABINSKÁ (703 Slovakia, belonging to the institution), Petr KACER (203 Czech Republic), Kamila SYSLOVA (203 Czech Republic), Alexandra ŠULCOVÁ (203 Czech Republic, belonging to the institution) and Magdalena SUSTKOVA-FISEROVA (203 Czech Republic)
Edition
The World Journal of Biological Psychiatry, Abingdon, Taylor & Francis LTD, 2015, 1562-2975
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30104 Pharmacology and pharmacy
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.159
RIV identification code
RIV/00216224:14110/15:00084203
Organization unit
Faculty of Medicine
UT WoS
000369761900007
Keywords in English
Depression; in vivo microdialysis; methamphetamine; olfactory bulbectomy; rats
Tags
International impact, Reviewed
Změněno: 8/4/2016 09:54, Ing. Mgr. Věra Pospíšilíková
V originále
OBJECTIVES: The self-medication hypothesis assumes that symptoms related to potential monoaminergic deficits in depression may be relieved by drug abuse. The aim of this study was to elucidate the neurotransmitter changes in a rat model of depression by measuring their levels in the nucleus accumbens shell, which is typically involved in the drug of abuse acquisition mechanism. METHODS: Depression was modelled by the olfactory bulbectomy (OBX) in Wistar male rats. In vivo microdialysis was performed, starting from the baseline and following after a single methamphetamine injection and behaviour was monitored. The determination of neurotransmitters and their metabolites was performed by high-performance liquid chromatography combined with mass spectrometry. RESULTS: OBX animals had lower basal levels of dopamine and serotonin and their metabolites. However, GABA and glutamate levels were increased. The methamphetamine injection induced stronger dopamine and serotonin release in the OBX rats and lower release of glutamate in comparison with sham-operated rats; GABA levels did not differ significantly. CONCLUSIONS: This study provides an evidence of mesolimbic neurotransmitter changes in the rat model of depression which may elucidate mechanisms underlying intravenous self-administration studies in which OBX rats were demonstrated to have higher drug intake in comparison to intact controls.
In Czech
Ve studii byly sledovány změny hladin dopaminu a jeho metabolitů v nucleus accumbens shell metodou in vivo mikrodialýzy u OBX a slepě operovaných zvířat po akutní dávce metamfetaminu. U OBX zvířat bylo zaznamenáno snížení bazálních hladin dopaminu a jeho metabolitů. Dávka metamfetaminu (5 mg/kg) však vyvolala u OBX ve srovnání s kontrolou vyšší vyplavení dopaminu a zvýšení koncentrace jeho metabolitů. Získaná data potvrzují odlišnou reaktivitu zvířat s depresi podobným fenotypem na psychostimulancia.
Links
| ED1.1.00/02.0068, research and development project |
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| MUNI/A/0886/2013, interní kód MU |
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| MUNI/A/1116/2014, interní kód MU |
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