Evaluation of the MALDI-TOF MS profiling for identification of
newly described Aeromonas spp.

J 2015

Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp.

VÁVROVÁ, Andrea, Tereza BALÁŽOVÁ, Ivo SEDLÁČEK, Ludmila TVRZOVÁ, Ondrej ŠEDO et. al.

Basic information

Original name

Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp.

Authors

VÁVROVÁ, Andrea (203 Czech Republic, guarantor, belonging to the institution), Tereza BALÁŽOVÁ (203 Czech Republic, belonging to the institution), Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution), Ludmila TVRZOVÁ (203 Czech Republic, belonging to the institution) and Ondrej ŠEDO (203 Czech Republic, belonging to the institution)

Edition

Folia Microbiologica, AV ČR, Institute of Microbiology, 2015, 0015-5632

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.335

RIV identification code

RIV/00216224:14310/15:00080481

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s12223-014-0369-4

UT WoS

000359398900002

Keywords in English

DESORPTION/IONIZATION-MASS SPECTROMETRY; GENUS AEROMONAS; SP-NOV.; PHYLOGENETIC ANALYSIS; HYDROPHILA; AQUARIORUM; WATER; ENTEROPELOGENES; BACTERIA; STRAINS

Abstract

V originále

The genus Aeromonas comprises primarily aquatic bacteria and also serious human and animal pathogens with the occurrence in clinical material, drinking water, and food. Aeromonads are typical for their complex taxonomy and nomenclature and for limited possibilities of identification to the species level. According to studies describing the use of MALDI-TOF MS in diagnostics of aeromonads, this modern chemotaxonomical approach reveals quite high percentage of correctly identified isolates. We analyzed 64 Aeromonas reference strains from the set of 27 species. After extending the range of analyzed Aeromonas species by newly described ones, we proved that MALDI-TOF MS procedure accompanied by Biotyper tool is not a reliable diagnostic technique for aeromonads. We obtained quite high percentage of false-positive, incorrect, and uncertain results. The identification of newly described species is accompanied with misidentifications that were observed also in the case of pathogenic aeromonads.

Links

CZ.1.05/1.1.00/02.0068, interní kód MU

Name: CEITEC - středoevropský technologický institut (Acronym: CEITEC)

Investor: Ministry of Education, Youth and Sports of the CR, CEITEC - Central European Institute of Technology, 1.1 European Centres of Excellence

CZ.1.07/2.3.00/20.0183, interní kód MU

Name: Centrum experimentální biomedicíny (Acronym: CEB)

Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development

MUNI/A/0884/2013, interní kód MU

Name: Podpora výzkumných aktivit studentů mikrobiologie (Acronym: MIKROB)

Investor: Masaryk University, Category A

QI101A094, research and development project

Name: Metody tlumení produkčních chorob skotu - BVD-MD a paratuberkulóza

Investor: Ministry of Agriculture of the CR, Sustainable development of agrarian sector

Citovat

VÁVROVÁ, Andrea, Tereza BALÁŽOVÁ, Ivo SEDLÁČEK, Ludmila TVRZOVÁ and Ondrej ŠEDO. Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp. Folia Microbiologica. AV ČR, Institute of Microbiology, 2015, vol. 60, No 5, p. 375-383. ISSN 0015-5632. Available from: https://dx.doi.org/10.1007/s12223-014-0369-4.

@article{1313800,
   author = {Vávrová, Andrea and Balážová, Tereza and Sedláček, Ivo and Tvrzová, Ludmila and Šedo, Ondrej},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s12223-014-0369-4},
   keywords = {DESORPTION/IONIZATION-MASS SPECTROMETRY; GENUS AEROMONAS; SP-NOV.; PHYLOGENETIC ANALYSIS; HYDROPHILA; AQUARIORUM; WATER; ENTEROPELOGENES; BACTERIA; STRAINS},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp.},
   volume = {60},
   year = {2015}
}

TY  - JOUR
ID  - 1313800
AU  - Vávrová, Andrea - Balážová, Tereza - Sedláček, Ivo - Tvrzová, Ludmila - Šedo, Ondrej
PY  - 2015
TI  - Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp.
JF  - Folia Microbiologica
VL  - 60
IS  - 5
SP  - 375-383
EP  - 375-383
PB  - AV ČR, Institute of Microbiology
SN  - 00155632
KW  - DESORPTION/IONIZATION-MASS SPECTROMETRY
KW  - GENUS AEROMONAS
KW  - SP-NOV.
KW  - PHYLOGENETIC ANALYSIS
KW  - HYDROPHILA
KW  - AQUARIORUM
KW  - WATER
KW  - ENTEROPELOGENES
KW  - BACTERIA
KW  - STRAINS
N2  - The genus Aeromonas comprises primarily aquatic bacteria and also serious human and animal pathogens with the occurrence in clinical material, drinking water, and food. Aeromonads are typical for their complex taxonomy and nomenclature and for limited possibilities of identification to the species level. According to studies describing the use of MALDI-TOF MS in diagnostics of aeromonads, this modern chemotaxonomical approach reveals quite high percentage of correctly identified isolates. We analyzed 64 Aeromonas reference strains from the set of 27 species. After extending the range of analyzed Aeromonas species by newly described ones, we proved that MALDI-TOF MS procedure accompanied by Biotyper tool is not a reliable diagnostic technique for aeromonads. We obtained quite high percentage of false-positive, incorrect, and uncertain results. The identification of newly described species is accompanied with misidentifications that were observed also in the case of pathogenic aeromonads.
ER  -

VÁVROVÁ, Andrea, Tereza BALÁŽOVÁ, Ivo SEDLÁČEK, Ludmila TVRZOVÁ and Ondrej ŠEDO. Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp. \textit{Folia Microbiologica}. AV ČR, Institute of Microbiology, 2015, vol.~60, No~5, p.~375-383. ISSN~0015-5632. Available from: https://dx.doi.org/10.1007/s12223-014-0369-4.

Detailed Information on Publication Record