DVOŘÁKOVÁ, Hana, Lucie JEDLIČKOVÁ, Martin KAŠNÝ, Jana ILGOVÁ, Pavel BROŽ, Hynek STRNAD, Roman LEONTOVIČ, Pavel ROUDNICKÝ, Jiří VOREL, Ewa DZIKA, Libor MIKEŠ and Milan GELNAR. Eudiplozoon nipponicum (Monogenea): Challenge for next-generation sequencing. In 9th International Symposium of Fish Parasites. 2015.
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Basic information
Original name Eudiplozoon nipponicum (Monogenea): Challenge for next-generation sequencing
Authors DVOŘÁKOVÁ, Hana (203 Czech Republic, guarantor), Lucie JEDLIČKOVÁ (203 Czech Republic), Martin KAŠNÝ (203 Czech Republic, belonging to the institution), Jana ILGOVÁ (703 Slovakia, belonging to the institution), Pavel BROŽ (203 Czech Republic), Hynek STRNAD (203 Czech Republic), Roman LEONTOVIČ (203 Czech Republic), Pavel ROUDNICKÝ (203 Czech Republic, belonging to the institution), Jiří VOREL (203 Czech Republic, belonging to the institution), Ewa DZIKA (616 Poland), Libor MIKEŠ (203 Czech Republic) and Milan GELNAR (203 Czech Republic, belonging to the institution).
Edition 9th International Symposium of Fish Parasites, 2015.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Spain
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/15:00081125
Organization unit Faculty of Science
Keywords in English Eudiplozoon nipponicum; Monogenea; next gene sequencing; transcriptome; genome
Changed by Changed by: Mgr. et Mgr. Jana Ilgová, Ph.D., učo 223075. Changed: 16/10/2015 12:01.
Abstract
Eudiplozoon nipponicum (family Diplozoidae, Polyopisthocotylea) is the hematophagous ectoparasite from the gills of cyprinid fish (Cyprinus carpio). The properties of genes (e.g. identity, structure, evolution) and proteins (e.g. function) of the members from the family Monogenea are among the less investigated in whole phylum Platyhelminthes. Up to now only two genomes and one transcriptome of monogenean species have been sequenced – genome of Protopolystoma xenopodis (Polyopisthocotylea) and Gyrodactylus salaris (Monopisthocotylea) and transcriptome of Neobenedenia melleni. Therefore we adopted the Illumina MiSeq/HiSeq techniques for sequencing the genomic DNA and RNA isolated from adult worms of E. nipponicum. This was followed by comprehensive bioinformatic analysis of sequential data.
Links
GAP506/12/1258, research and development projectName: Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu
Investor: Czech Science Foundation
GBP505/12/G112, research and development projectName: ECIP - Evropské centrum ichtyoparazitologie
Investor: Czech Science Foundation
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