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@article{1315372,
author = {Horká, Marie and Tesařová, Marie and Karásek, Pavel and Růžička, Filip and Holá, Veronika and Sittová, Martina and Roth, Michal},
article_location = {Amsterdam},
article_number = {APR 8 2015},
doi = {http://dx.doi.org/10.1016/j.aca.2015.02.001},
keywords = {Capillary electrophoresis; Supercritical water; FS capillary; Methicillin-resistant Staphylococcus aureus - MRSA; Methicillin-susceptible Staphylococcus aureus - MSSA; Whole human blood},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {Determination of methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteria in blood by capillary zone electrophoresis},
volume = {868},
year = {2015}
}
TY - JOUR
ID - 1315372
AU - Horká, Marie - Tesařová, Marie - Karásek, Pavel - Růžička, Filip - Holá, Veronika - Sittová, Martina - Roth, Michal
PY - 2015
TI - Determination of methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteria in blood by capillary zone electrophoresis
JF - Analytica Chimica Acta
VL - 868
IS - APR 8 2015
SP - 67-72
EP - 67-72
PB - Elsevier Science publishers
SN - 00032670
KW - Capillary electrophoresis
KW - Supercritical water
KW - FS capillary
KW - Methicillin-resistant Staphylococcus aureus - MRSA
KW - Methicillin-susceptible Staphylococcus aureus - MSSA
KW - Whole human blood
N2 - Serious bloodstream infections are a significant complication in critically ill patients. The treatment of these infections has become more difficult because of the increasing prevalence of multiresistant strains, especially methicillin-resistant Staphylococcus aureus (MRSA). Rapid differentiation of low number of MRSA from methicillin-susceptible S. aureus (MSSA) cells (10(1)-10(2) cells mL (1)) in blood is necessary for fast effective antibiotic therapy. Currently, three groups of techniques, phenotyping, genotyping, and mass spectrometry, are used for MRSA and MSSA strains differentiation. Most of these techniques are time-consuming. PCR and other molecular techniques allow the detection and differentiation between MSSA and MRSA directly from blood cultures. These methods alone are rapid and they have good reproducibility and repeatability. Potential disadvantages of the genotyping methods include their discrimination ability, technical complexity, financial costs, and difficult interpretation of the results. Recently, capillary electrophoresis (CZE) was successfully used to differentiate between the agar-cultivated MRSA and MSSA strains in fused silica capillaries etched with supercritical water and modified with (3-glycidyloxypropyl) trimethoxysilane. The possible use of CZE as a fast and low-cost method for distinguishing between the blood-incubated MRSA or MSSA cells has been tested in this manuscript. Our goal was to test low amounts of bacteria (similar to 10(2) cell mL (1)) similar to those in clinical samples. The migration times of the purified blood-incubated cells and the agar-cultivated cells were different from each other. However, their isoelectric point was the same for all strains.
ER -
HORKÁ, Marie, Marie TESAŘOVÁ, Pavel KARÁSEK, Filip RŮŽIČKA, Veronika HOLÁ, Martina SITTOVÁ and Michal ROTH. Determination of methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteria in blood by capillary zone electrophoresis. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science publishers, 2015, vol.~868, APR 8 2015, p.~67-72. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2015.02.001.
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