Auxin-binding pocket of ABP1 is crucial for its
gain-of-function cellular and developmental roles

J 2015

Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles

GRONES, Peter, Xu CHEN, Sibu SIMON, Walter A. KAUFMANN, Riet DE RYCKE et. al.

Basic information

Original name

Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles

Authors

GRONES, Peter (56 Belgium), Xu CHEN (56 Belgium), Sibu SIMON (56 Belgium), Walter A. KAUFMANN (56 Belgium), Riet DE RYCKE (56 Belgium), Tomasz NODZYNSKI (616 Poland, guarantor, belonging to the institution), Eva ZAŽÍMALOVÁ (203 Czech Republic) and Jiří FRIML (203 Czech Republic)

Edition

Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2015, 0022-0957

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.677

RIV identification code

RIV/00216224:14740/15:00081165

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/jxb/erv177

UT WoS

000359688300017

Keywords in English

Auxin; ABP1; Auxin binding; Site-directed mutagenesis; PIN proteins

Abstract

V originále

The plant hormone auxin is a key regulator of plant growth and development. Auxin levels are sensed and interpreted by distinct receptor systems that activate a broad range of cellular responses. The Auxin-Binding Protein1 (ABP1) that has been identified based on its ability to bind auxin with high affinity is a prime candidate for the extracellular receptor responsible for mediating a range of auxin effects, in particular, the fast non-transcriptional ones. Contradictory genetic studies suggested prominent or no importance of ABP1 in many developmental processes. However, how crucial the role of auxin binding to ABP1 is for its functions has not been addressed. Here, we show that the auxinbinding pocket of ABP1 is essential for its gain-of-function cellular and developmental roles. In total, 16 different abp1 mutants were prepared that possessed substitutions in the metal core or in the hydrophobic amino acids of the auxin-binding pocket as well as neutral mutations. Their analysis revealed that an intact auxin-binding pocket is a prerequisite for ABP1 to activate downstream components of the ABP1 signalling pathway, such as Rho of Plants (ROPs) and to mediate the clathrin association with membranes for endocytosis regulation. In planta analyses demonstrated the importance of the auxin binding pocket for all known ABP1-mediated postembryonic developmental processes, including morphology of leaf epidermal cells, root growth and root meristem activity, and vascular tissue differentiation. Taken together, these findings suggest that auxin binding to ABP1 is central to its function, supporting the role of ABP1 as auxin receptor.

Links

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GA13-40637S, research and development project

Name: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Czech Science Foundation

Citovat

GRONES, Peter, Xu CHEN, Sibu SIMON, Walter A. KAUFMANN, Riet DE RYCKE, Tomasz NODZYNSKI, Eva ZAŽÍMALOVÁ and Jiří FRIML. Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. Journal of Experimental Botany. Oxford: OXFORD UNIV PRESS, 2015, vol. 66, No 16, p. 5055-5065. ISSN 0022-0957. Available from: https://dx.doi.org/10.1093/jxb/erv177.

@article{1315668,
   author = {Grones, Peter and Chen, Xu and Simon, Sibu and Kaufmann, Walter A. and De Rycke, Riet and Nodzynski, Tomasz and Zažímalová, Eva and Friml, Jiří},
   article_location = {Oxford},
   article_number = {16},
   doi = {http://dx.doi.org/10.1093/jxb/erv177},
   keywords = {Auxin; ABP1; Auxin binding; Site-directed mutagenesis; PIN proteins},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles},
   url = {http://jxb.oxfordjournals.org/content/66/16/5055.abstract},
   volume = {66},
   year = {2015}
}

TY  - JOUR
ID  - 1315668
AU  - Grones, Peter - Chen, Xu - Simon, Sibu - Kaufmann, Walter A. - De Rycke, Riet - Nodzynski, Tomasz - Zažímalová, Eva - Friml, Jiří
PY  - 2015
TI  - Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles
JF  - Journal of Experimental Botany
VL  - 66
IS  - 16
SP  - 5055-5065
EP  - 5055-5065
PB  - OXFORD UNIV PRESS
SN  - 00220957
KW  - Auxin
KW  - ABP1
KW  - Auxin binding
KW  - Site-directed mutagenesis
KW  - PIN proteins
UR  - http://jxb.oxfordjournals.org/content/66/16/5055.abstract
L2  - http://jxb.oxfordjournals.org/content/66/16/5055.abstract
N2  - The plant hormone auxin is a key regulator of plant growth and development. Auxin levels are sensed and interpreted by distinct receptor systems that activate a broad range of cellular responses. The Auxin-Binding Protein1 (ABP1) that has been identified based on its ability to bind auxin with high affinity is a prime candidate for the extracellular receptor responsible for mediating a range of auxin effects, in particular, the fast non-transcriptional ones. Contradictory genetic studies suggested prominent or no importance of ABP1 in many developmental processes. However, how crucial the role of auxin binding to ABP1 is for its functions has not been addressed. Here, we show that the auxinbinding pocket of ABP1 is essential for its gain-of-function cellular and developmental roles. In total, 16 different abp1 mutants were prepared that possessed substitutions in the metal core or in the hydrophobic amino acids of the auxin-binding pocket as well as neutral mutations. Their analysis revealed that an intact auxin-binding pocket is a prerequisite for ABP1 to activate downstream components of the ABP1 signalling pathway, such as Rho of Plants (ROPs) and to mediate the clathrin association with membranes for endocytosis regulation. In planta analyses demonstrated the importance of the auxin binding pocket for all known ABP1-mediated postembryonic developmental processes, including morphology of leaf epidermal cells, root growth and root meristem activity, and vascular tissue differentiation. Taken together, these findings suggest that auxin binding to ABP1 is central to its function, supporting the role of ABP1 as auxin receptor.
ER  -

GRONES, Peter, Xu CHEN, Sibu SIMON, Walter A. KAUFMANN, Riet DE RYCKE, Tomasz NODZYNSKI, Eva ZAŽÍMALOVÁ and Jiří FRIML. Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. \textit{Journal of Experimental Botany}. Oxford: OXFORD UNIV PRESS, 2015, vol.~66, No~16, p.~5055-5065. ISSN~0022-0957. Available from: https://dx.doi.org/10.1093/jxb/erv177.

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