Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana

ZWIEWKA, Marta, Tomasz NODZYNSKI, Stephanie ROBERT, Steffen VANNESTE and Jiří FRIML. Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana. Online. Molecular Plant. Cambridge (USA): Cell Press, 2015, vol. 8, No 8, p. 1175-1187. ISSN 1674-2052. Available from: https://dx.doi.org/10.1016/j.molp.2015.03.007. [citováno 2024-04-24]

Basic information

• Original name: Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana
• Authors: ZWIEWKA, Marta (616 Poland, belonging to the institution), Tomasz NODZYNSKI (616 Poland, belonging to the institution), Stephanie ROBERT (56 Belgium), Steffen VANNESTE (56 Belgium) and Jiří FRIML (203 Czech Republic, guarantor, belonging to the institution)
• Edition: Molecular Plant, Cambridge (USA), Cell Press, 2015, 1674-2052.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 7.142
• RIV identification code: RIV/00216224:14740/15:00081166
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1016/j.molp.2015.03.007
• UT WoS: 000359136100006
• Keywords in English: osmotic stress; protein trafficking; clathrin-mediated endocytosis; auxin
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

The sessile life style of plants creates the need to deal with an often adverse environment, in which water availability can change on a daily basis, challenging the cellular physiology and integrity. Changes in osmotic conditions disrupt the equilibrium of the plasma membrane: hypoosmotic conditions increase and hyperosmotic environment decrease the cell volume. Here, we show that short-term extracellular osmotic treatments are closely followed by a shift in the balance between endocytosis and exocytosis in root meristem cells. Acute hyperosmotic treatments (ionic and nonionic) enhance clathrin-mediated endocytosis simultaneously attenuating exocytosis, whereas hypoosmotic treatments have the opposite effects. In addition to clathrin recruitment to the plasma membrane, components of early endocytic trafficking are essential during hyperosmotic stress responses. Consequently, growth of seedlings defective in elements of clathrin or early endocytic machinery is more sensitive to hyperosmotic treatments. We also found that the endocytotic response to a change of osmotic status in the environment is dominant over the presumably evolutionary more recent regulatory effect of plant hormones, such as auxin. These results imply that osmotic perturbation influences the balance between endocytosis and exocytosis acting through clathrin-mediated endocytosis. We propose that tension on the plasma membrane determines the addition or removal of membranes at the cell surface, thus preserving cell integrity.

Links

• EE2.3.20.0043, research and development project: Name: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů
• EE2.3.30.0009, research and development project: Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci
• GA13-40637S, research and development project: Name: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Czech Science Foundation