| POSPÍCHALOVÁ, Vendula, J TURECKOVA, Bohumil FAFÍLEK, M VOJTECHOVA, M KRAUSOVA, J LUKAS, E SLONCOVA, Sylvia TAKÁČOVÁ, Vladimír DIVOKÝ, D LEPRINCE, J PLACHY a V KORINEK. Generation of Two Modified Mouse Alleles of the Hic1 Tumor Suppressor Gene. Online. GENESIS. MALDEN: WILEY-BLACKWELL, 2011, roč. 49, č. 3, s. 142-151. ISSN 1526-954X. Dostupné z: https://dx.doi.org/10.1002/dvg.20719. [citováno 2024-04-24] |
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@article{1316071,
author = {Pospíchalová, Vendula and Tureckova, J and Fafílek, Bohumil and Vojtechova, M and Krausova, M and Lukas, J and Sloncova, E and Takáčová, Sylvia and Divoký, Vladimír and Leprince, D and Plachy, J and Korinek, V},
article_location = {MALDEN},
article_number = {3},
doi = {http://dx.doi.org/10.1002/dvg.20719},
keywords = {hypermethylated in cancer 1; Hic1 tumor suppressor; gene targeting; a conditional null allele; citrine reporter mice; placenta},
language = {eng},
issn = {1526-954X},
journal = {GENESIS},
title = {Generation of Two Modified Mouse Alleles of the Hic1 Tumor Suppressor Gene},
volume = {49},
year = {2011}
}
TY - JOUR
ID - 1316071
AU - Pospíchalová, Vendula - Tureckova, J - Fafílek, Bohumil - Vojtechova, M - Krausova, M - Lukas, J - Sloncova, E - Takáčová, Sylvia - Divoký, Vladimír - Leprince, D - Plachy, J - Korinek, V
PY - 2011
TI - Generation of Two Modified Mouse Alleles of the Hic1 Tumor Suppressor Gene
JF - GENESIS
VL - 49
IS - 3
SP - 142-151
EP - 142-151
PB - WILEY-BLACKWELL
SN - 1526954X
KW - hypermethylated in cancer 1
KW - Hic1 tumor suppressor
KW - gene targeting
KW - a conditional null allele
KW - citrine reporter mice
KW - placenta
N2 - HIC1 (hypermethylated in cancer 1) is a tumor suppressor gene located on chromosome 17p13.3, a region frequently hypermethylated or deleted in human neoplasias. In mouse, Hic1 is essential for embryonic development and exerts an antitumor role in adult animals. Since Hic1-deficient mice die perinatally, we generated a conditional Hic1 null allele by flanking the Hic1-coding region by loxP sites. When crossed to animals expressing Cre recombinase in a cell-specific manner, the Hic1 conditional mice will provide new insights into the function of Hic1 in developing and mature tissues. Additionally, we used gene targeting to replace sequence-encoding amino acids 186-893 of Hic1 by citrine fluorescent protein cDNA. We demonstrate that the distribution of Hic1-citrine fusion polypeptide corresponds to the expression pattern of wild-type Hic1. Consequently, Hic1-citrine "reporter'' mice can be used to monitor the activity of the Hic1 locus using citrine fluorescence. genesis 49:142-151, 2011. (C) 2011 Wiley-Liss, Inc.
ER -
POSPÍCHALOVÁ, Vendula, J TURECKOVA, Bohumil FAFÍLEK, M VOJTECHOVA, M KRAUSOVA, J LUKAS, E SLONCOVA, Sylvia TAKÁČOVÁ, Vladimír DIVOKÝ, D LEPRINCE, J PLACHY a V KORINEK. Generation of Two Modified Mouse Alleles of the Hic1 Tumor Suppressor Gene. Online. \textit{GENESIS}. MALDEN: WILEY-BLACKWELL, 2011, roč.~49, č.~3, s.~142-151. ISSN~1526-954X. Dostupné z: https://dx.doi.org/10.1002/dvg.20719. [citováno 2024-04-24]
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