Proteome and transcript analysis of Vitis vinifera cell
cultures subjected to Botrytis cinerea infection

J 2015

Proteome and transcript analysis of Vitis vinifera cell cultures subjected to Botrytis cinerea infection

DADÁKOVÁ, Kateřina, Martina GROCHALOVÁ, Barbora KURKOVÁ, Ivana TLOLKOVÁ, Tomáš KAŠPAROVSKÝ et. al.

Basic information

Original name

Proteome and transcript analysis of Vitis vinifera cell cultures subjected to Botrytis cinerea infection

Authors

DADÁKOVÁ, Kateřina (203 Czech Republic, belonging to the institution), Martina GROCHALOVÁ (203 Czech Republic, belonging to the institution), Barbora KURKOVÁ (203 Czech Republic, belonging to the institution), Ivana TLOLKOVÁ (203 Czech Republic, belonging to the institution), Tomáš KAŠPAROVSKÝ (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution) and Jan LOCHMAN (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Proteomics, Amsterdam, Elsevier Science, 2015, 1874-3919

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.867

RIV identification code

RIV/00216224:14310/15:00081187

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.jprot.2015.02.001

UT WoS

000354344100012

Keywords in English

Vitis vinifera; Botrytis cinerea; Defence; Cytoplasmic proteome; Vacuolar proteome; Transcript analysis

Abstract

V originále

Gray mold caused by Botrytis cinerea is one of the most important diseases of grapevine resulting in significant reductions in yield and fruit quality. In order to examine the molecular mechanisms that characterize the interaction between B. cinerea and the host plant, the grapevine cytoplasmic proteome was analyzed by two-dimensional polyacrylamide gel electrophoresis. The interaction between Vitis vinifera cv. Gamay cells and B. cinerea was characterized by the increase in spot abundance of 30 proteins, of which 21 were successfully identified. The majority of these proteins were related to defence and stress responses and to cell wall modifications. Some of the modulated proteins have been previously found to be affected by other pathogens when they infect V. vinifera but interestingly, the proteins related to cell wall modification that were influenced by B. cinerea have not been shown to be modulated by any other pathogen studied to date. Transcript analysis using the quantitative real time polymerase chain reaction additionally revealed the up-regulation of several acidic, probably extracellular, chitinases. The results indicate that cell wall strengthening, accumulation of PR proteins and excretion of lytic enzymes are likely to be important mechanisms in the defence of grapevine against B. cinerea. Biological significance Although gray mold caused by Botrytis cinerea is one of the most important diseases of grapevine, little information is available about proteomic changes in this pathosystem. These results suggest that cell wall strengthening, accumulation of PR proteins and excretion of lytic enzymes are important molecular mechanisms in the defence of grapevine against B. cinerea. Surprisingly, the proteins related to cell wall modification that were modulated by B. cinerea have not been shown to be affected by any other pathogen studied to date. (C) 2015 Elsevier B.V. All rights reserved.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GAP501/12/0590, research and development project

Name: Charakterizace procesů zapojených v indukci resistence rostlin na patogeny s využitím elicitinů se změněnou schopností vyvolávat obranné reakce

Investor: Czech Science Foundation

GBP206/12/G151, research and development project

Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

DADÁKOVÁ, Kateřina, Martina GROCHALOVÁ, Barbora KURKOVÁ, Ivana TLOLKOVÁ, Tomáš KAŠPAROVSKÝ, Zbyněk ZDRÁHAL and Jan LOCHMAN. Proteome and transcript analysis of Vitis vinifera cell cultures subjected to Botrytis cinerea infection. Journal of Proteomics. Amsterdam: Elsevier Science, 2015, vol. 119, April, p. 143-153. ISSN 1874-3919. Available from: https://dx.doi.org/10.1016/j.jprot.2015.02.001.

@article{1316836,
   author = {Dadáková, Kateřina and Grochalová, Martina and Kurková, Barbora and Tlolková, Ivana and Kašparovský, Tomáš and Zdráhal, Zbyněk and Lochman, Jan},
   article_location = {Amsterdam},
   article_number = {April},
   doi = {http://dx.doi.org/10.1016/j.jprot.2015.02.001},
   keywords = {Vitis vinifera; Botrytis cinerea; Defence; Cytoplasmic proteome; Vacuolar proteome; Transcript analysis},
   language = {eng},
   issn = {1874-3919},
   journal = {Journal of Proteomics},
   title = {Proteome and transcript analysis of Vitis vinifera cell cultures subjected to Botrytis cinerea infection},
   volume = {119},
   year = {2015}
}

TY  - JOUR
ID  - 1316836
AU  - Dadáková, Kateřina - Grochalová, Martina - Kurková, Barbora - Tlolková, Ivana - Kašparovský, Tomáš - Zdráhal, Zbyněk - Lochman, Jan
PY  - 2015
TI  - Proteome and transcript analysis of Vitis vinifera cell cultures subjected to Botrytis cinerea infection
JF  - Journal of Proteomics
VL  - 119
IS  - April
SP  - 143-153
EP  - 143-153
PB  - Elsevier Science
SN  - 18743919
KW  - Vitis vinifera
KW  - Botrytis cinerea
KW  - Defence
KW  - Cytoplasmic proteome
KW  - Vacuolar proteome
KW  - Transcript analysis
N2  - Gray mold caused by Botrytis cinerea is one of the most important diseases of grapevine resulting in significant reductions in yield and fruit quality. In order to examine the molecular mechanisms that characterize the interaction between B. cinerea and the host plant, the grapevine cytoplasmic proteome was analyzed by two-dimensional polyacrylamide gel electrophoresis. The interaction between Vitis vinifera cv. Gamay cells and B. cinerea was characterized by the increase in spot abundance of 30 proteins, of which 21 were successfully identified. The majority of these proteins were related to defence and stress responses and to cell wall modifications. Some of the modulated proteins have been previously found to be affected by other pathogens when they infect V. vinifera but interestingly, the proteins related to cell wall modification that were influenced by B. cinerea have not been shown to be modulated by any other pathogen studied to date. Transcript analysis using the quantitative real time polymerase chain reaction additionally revealed the up-regulation of several acidic, probably extracellular, chitinases. The results indicate that cell wall strengthening, accumulation of PR proteins and excretion of lytic enzymes are likely to be important mechanisms in the defence of grapevine against B. cinerea. Biological significance Although gray mold caused by Botrytis cinerea is one of the most important diseases of grapevine, little information is available about proteomic changes in this pathosystem. These results suggest that cell wall strengthening, accumulation of PR proteins and excretion of lytic enzymes are important molecular mechanisms in the defence of grapevine against B. cinerea. Surprisingly, the proteins related to cell wall modification that were modulated by B. cinerea have not been shown to be affected by any other pathogen studied to date. (C) 2015 Elsevier B.V. All rights reserved.
ER  -

DADÁKOVÁ, Kateřina, Martina GROCHALOVÁ, Barbora KURKOVÁ, Ivana TLOLKOVÁ, Tomáš KAŠPAROVSKÝ, Zbyněk ZDRÁHAL and Jan LOCHMAN. Proteome and transcript analysis of Vitis vinifera cell cultures subjected to Botrytis cinerea infection. \textit{Journal of Proteomics}. Amsterdam: Elsevier Science, 2015, vol.~119, April, p.~143-153. ISSN~1874-3919. Available from: https://dx.doi.org/10.1016/j.jprot.2015.02.001.

Detailed Information on Publication Record