Determinants encoding fimbriae type 1 in fecal Escherichia coli
are associated with increased frequency of bacteriocinogeny

J 2015

Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny

ŠTAUDOVÁ, Barbora, Lenka MICENKOVÁ, Juraj BOSÁK, Kristýna HRAZDILOVÁ, Eva SLANINKOVÁ et. al.

Basic information

Original name

Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny

Authors

ŠTAUDOVÁ, Barbora (203 Czech Republic, belonging to the institution), Lenka MICENKOVÁ (703 Slovakia, belonging to the institution), Juraj BOSÁK (703 Slovakia, belonging to the institution), Kristýna HRAZDILOVÁ (203 Czech Republic), Eva SLANINKOVÁ (203 Czech Republic), Martin VRBA (203 Czech Republic), Alena ŠEVČÍKOVÁ (203 Czech Republic, belonging to the institution), Darina KOHOUTOVÁ (203 Czech Republic), Vladana WOZNICOVÁ (203 Czech Republic, belonging to the institution), Jan BUREŠ (203 Czech Republic) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

BMC Microbiology, London, BioMed Central, 2015, 1471-2180

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.581

RIV identification code

RIV/00216224:14110/15:00087463

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1186/s12866-015-0530-5

UT WoS

000362251700001

Keywords in English

Escherichia coli; Colicin; Microcin; Bacteriocin; Type 1 fimbriae; Phylogenetic group

Abstract

V originále

Background: To screen whether E. coli strains encoding type 1 fimbriae, isolated from fecal microflora, produce bacteriocins more often relative to fimA-negative E. coli strains of similar origin. Methods: PCR assays were used to detect presence of genes encoding 30 bacteriocin determinants (23 colicin-and 7 microcin-encoding genes) and 18 virulence determinants in 579 E. coli strains of human and animal origin isolated from hospitals and animal facilities in the Czech and Slovak Republic. E. coli strains were also classified into phylogroups (A, B1, B2 and D). Results: fimA-negative E. coli strains (defined as those possessing none of the 18 tested virulence determinants) were compared to fimA-positive E. coli strains (possessing fimA as the only detected virulence determinant). Strains with identified bacteriocin genes were more commonly found among fimA-positive E. coli strains (35.6 %) compared to fimA-negative E. coli strains (21.9 %, p < 0.01) and this was true for both colicin and microcin determinants (p = 0.02 and p < 0.01, respectively). In addition, an increased number of strains encoding colicin E1 were found among fimA-positive E. coli strains (p < 0.01). Conclusions: fimA-positive E. coli strains produced bacteriocins (colicins and microcins) more often compared to fimA-negative strains of similar origin. Since type 1 fimbriae of E. coli have been shown to mediate adhesion to epithelial host cells and help colonize the intestines, bacteriocin synthesis appears to be an additional feature of colonizing E. coli strains.

Links

NT13413, research and development project

Name: Stanovení apoptózy v biopticky odebraných vzorcích z tlustého střeva

Investor: Ministry of Health of the CR

Citovat

ŠTAUDOVÁ, Barbora, Lenka MICENKOVÁ, Juraj BOSÁK, Kristýna HRAZDILOVÁ, Eva SLANINKOVÁ, Martin VRBA, Alena ŠEVČÍKOVÁ, Darina KOHOUTOVÁ, Vladana WOZNICOVÁ, Jan BUREŠ and David ŠMAJS. Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny. BMC Microbiology. London: BioMed Central, 2015, vol. 15, No 201, p. 1-9. ISSN 1471-2180. Available from: https://dx.doi.org/10.1186/s12866-015-0530-5.

@article{1317917,
   author = {Štaudová, Barbora and Micenková, Lenka and Bosák, Juraj and Hrazdilová, Kristýna and Slaninková, Eva and Vrba, Martin and Ševčíková, Alena and Kohoutová, Darina and Woznicová, Vladana and Bureš, Jan and Šmajs, David},
   article_location = {London},
   article_number = {201},
   doi = {http://dx.doi.org/10.1186/s12866-015-0530-5},
   keywords = {Escherichia coli; Colicin; Microcin; Bacteriocin; Type 1 fimbriae; Phylogenetic group},
   language = {eng},
   issn = {1471-2180},
   journal = {BMC Microbiology},
   title = {Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny},
   volume = {15},
   year = {2015}
}

TY  - JOUR
ID  - 1317917
AU  - Štaudová, Barbora - Micenková, Lenka - Bosák, Juraj - Hrazdilová, Kristýna - Slaninková, Eva - Vrba, Martin - Ševčíková, Alena - Kohoutová, Darina - Woznicová, Vladana - Bureš, Jan - Šmajs, David
PY  - 2015
TI  - Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny
JF  - BMC Microbiology
VL  - 15
IS  - 201
SP  - 1-9
EP  - 1-9
PB  - BioMed Central
SN  - 14712180
KW  - Escherichia coli
KW  - Colicin
KW  - Microcin
KW  - Bacteriocin
KW  - Type 1 fimbriae
KW  - Phylogenetic group
N2  - Background: To screen whether E. coli strains encoding type 1 fimbriae, isolated from fecal microflora, produce bacteriocins more often relative to fimA-negative E. coli strains of similar origin. Methods: PCR assays were used to detect presence of genes encoding 30 bacteriocin determinants (23 colicin-and 7 microcin-encoding genes) and 18 virulence determinants in 579 E. coli strains of human and animal origin isolated from hospitals and animal facilities in the Czech and Slovak Republic. E. coli strains were also classified into phylogroups (A, B1, B2 and D). Results: fimA-negative E. coli strains (defined as those possessing none of the 18 tested virulence determinants) were compared to fimA-positive E. coli strains (possessing fimA as the only detected virulence determinant). Strains with identified bacteriocin genes were more commonly found among fimA-positive E. coli strains (35.6 %) compared to fimA-negative E. coli strains (21.9 %, p < 0.01) and this was true for both colicin and microcin determinants (p = 0.02 and p < 0.01, respectively). In addition, an increased number of strains encoding colicin E1 were found among fimA-positive E. coli strains (p < 0.01). Conclusions: fimA-positive E. coli strains produced bacteriocins (colicins and microcins) more often compared to fimA-negative strains of similar origin. Since type 1 fimbriae of E. coli have been shown to mediate adhesion to epithelial host cells and help colonize the intestines, bacteriocin synthesis appears to be an additional feature of colonizing E. coli strains.
ER  -

ŠTAUDOVÁ, Barbora, Lenka MICENKOVÁ, Juraj BOSÁK, Kristýna HRAZDILOVÁ, Eva SLANINKOVÁ, Martin VRBA, Alena ŠEVČÍKOVÁ, Darina KOHOUTOVÁ, Vladana WOZNICOVÁ, Jan BUREŠ and David ŠMAJS. Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny. \textit{BMC Microbiology}. London: BioMed Central, 2015, vol.~15, No~201, p.~1-9. ISSN~1471-2180. Available from: https://dx.doi.org/10.1186/s12866-015-0530-5.

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