Introduction of macarpine as a novel cell-permeant DNA dye for
live cell imaging and flow cytometry sorting

J 2016

Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting

SLANINOVÁ, Iva; Noelia LÓPEZ-SÁNCHEZ; Kristýna ŠEBRLOVÁ; Ondřej VYMAZAL; José María FRADE et. al.

Basic information

Original name

Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting

Authors

SLANINOVÁ, Iva (203 Czech Republic, guarantor, belonging to the institution); Noelia LÓPEZ-SÁNCHEZ (724 Spain); Kristýna ŠEBRLOVÁ (203 Czech Republic, belonging to the institution); Ondřej VYMAZAL (203 Czech Republic, belonging to the institution); José María FRADE (724 Spain) and Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution)

Edition

Biology of the Cell, Hoboken, Wiley-Blackwell, 2016, 0248-4900

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 2.649

RIV identification code

RIV/00216224:14110/16:00089182

Organization unit

Faculty of Medicine

DOI

https://doi.org/10.1111/boc.201500047

UT WoS

000369960200001

EID Scopus

2-s2.0-84955758572

Keywords in English

Cell cycle; DNA dye; FACS; Macarpine; Time-lapse imaging

Abstract

In the original language

Macarpine (MA) is a quaternary benzophenanthridine plant alkaloid isolated from Macleaya microcarpa or Stylophorum lasiocarpum. Benzophenanthridine alkaloids are interesting natural products that display antiproliferative, antimicrobial, antifungal and anti-inflammatory activities, and also fluorescence properties. In a previous study, we demonstrated that thanks to its ability to interact with DNA and its spectral properties MA could be used as a supravital DNA probe for fluorescence microscopy and flow cytometry including analyses of the cell cycle. In this study, we evaluated the suitability of MA as a DNA dye for time-lapse microscopy and flow-cytometric cell sorting.

Links

LH12176, research and development project

Name: Benzofenanthridinové alkaloidy - studium účinků na celulární a molekulární úrovni (Acronym: KBF)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1123/2014, interní kód MU

Name: Molekulární a buněčná biomedicína (Acronym: MBB)

Investor: Masaryk University, Category A

MUNI/A/1195/2014, interní kód MU

Name: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: chebimo)

Investor: Masaryk University, Category A

Cite

SLANINOVÁ, Iva; Noelia LÓPEZ-SÁNCHEZ; Kristýna ŠEBRLOVÁ; Ondřej VYMAZAL; José María FRADE and Eva TÁBORSKÁ. Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting. Biology of the Cell. Hoboken: Wiley-Blackwell, 2016, vol. 108, No 1, p. 1-18. ISSN 0248-4900. Available from: https://doi.org/10.1111/boc.201500047.

@article{1318390,
   author = {Slaninová, Iva and LópezandSánchez, Noelia and Šebrlová, Kristýna and Vymazal, Ondřej and Frade, José María and Táborská, Eva},
   article_location = {Hoboken},
   article_number = {1},
   doi = {https://doi.org/10.1111/boc.201500047},
   keywords = {Cell cycle; DNA dye; FACS; Macarpine; Time-lapse imaging},
   language = {eng},
   issn = {0248-4900},
   journal = {Biology of the Cell},
   title = {Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting},
   volume = {108},
   year = {2016}
}

TY  - JOUR
ID  - 1318390
AU  - Slaninová, Iva - López-Sánchez, Noelia - Šebrlová, Kristýna - Vymazal, Ondřej - Frade, José María - Táborská, Eva
PY  - 2016
TI  - Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting
JF  - Biology of the Cell
VL  - 108
IS  - 1
SP  - 1-18
EP  - 1-18
PB  - Wiley-Blackwell
SN  - 02484900
KW  - Cell cycle
KW  - DNA dye
KW  - FACS
KW  - Macarpine
KW  - Time-lapse imaging
N2  - Macarpine (MA) is a quaternary benzophenanthridine plant alkaloid isolated from Macleaya microcarpa or Stylophorum lasiocarpum. Benzophenanthridine alkaloids are interesting natural products that display antiproliferative, antimicrobial, antifungal and anti-inflammatory activities, and also fluorescence properties. In a previous study, we demonstrated that thanks to its ability to interact with DNA and its spectral properties MA could be used as a supravital DNA probe for fluorescence microscopy and flow cytometry including analyses of the cell cycle. In this study, we evaluated the suitability of MA as a DNA dye for time-lapse microscopy and flow-cytometric cell sorting.
ER  -

SLANINOVÁ, Iva; Noelia LÓPEZ-SÁNCHEZ; Kristýna ŠEBRLOVÁ; Ondřej VYMAZAL; José María FRADE and Eva TÁBORSKÁ. Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting. \textit{Biology of the Cell}. Hoboken: Wiley-Blackwell, 2016, vol.~108, No~1, p.~1-18. ISSN~0248-4900. Available from: https://doi.org/10.1111/boc.201500047.

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