Introduction of macarpine as a novel cell-permeant DNA dye for
live cell imaging and flow cytometry sorting

J 2016

Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting

SLANINOVÁ, Iva, Noelia LÓPEZ-SÁNCHEZ, Kristýna ŠEBRLOVÁ, Ondřej VYMAZAL, José María FRADE et. al.

Basic information

Original name

Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting

Authors

SLANINOVÁ, Iva (203 Czech Republic, guarantor, belonging to the institution), Noelia LÓPEZ-SÁNCHEZ (724 Spain), Kristýna ŠEBRLOVÁ (203 Czech Republic, belonging to the institution), Ondřej VYMAZAL (203 Czech Republic, belonging to the institution), José María FRADE (724 Spain) and Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution)

Edition

Biology of the Cell, Hoboken, Wiley-Blackwell, 2016, 0248-4900

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.649

RIV identification code

RIV/00216224:14110/16:00089182

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1111/boc.201500047

UT WoS

000369960200001

Keywords in English

Cell cycle; DNA dye; FACS; Macarpine; Time-lapse imaging

Abstract

V originále

Macarpine (MA) is a quaternary benzophenanthridine plant alkaloid isolated from Macleaya microcarpa or Stylophorum lasiocarpum. Benzophenanthridine alkaloids are interesting natural products that display antiproliferative, antimicrobial, antifungal and anti-inflammatory activities, and also fluorescence properties. In a previous study, we demonstrated that thanks to its ability to interact with DNA and its spectral properties MA could be used as a supravital DNA probe for fluorescence microscopy and flow cytometry including analyses of the cell cycle. In this study, we evaluated the suitability of MA as a DNA dye for time-lapse microscopy and flow-cytometric cell sorting.

Links

LH12176, research and development project

Name: Benzofenanthridinové alkaloidy - studium účinků na celulární a molekulární úrovni (Acronym: KBF)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1123/2014, interní kód MU

Name: Molekulární a buněčná biomedicína (Acronym: MBB)

Investor: Masaryk University, Category A

MUNI/A/1195/2014, interní kód MU

Name: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: chebimo)

Investor: Masaryk University, Category A

Citovat

SLANINOVÁ, Iva, Noelia LÓPEZ-SÁNCHEZ, Kristýna ŠEBRLOVÁ, Ondřej VYMAZAL, José María FRADE and Eva TÁBORSKÁ. Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting. Biology of the Cell. Hoboken: Wiley-Blackwell, 2016, vol. 108, No 1, p. 1-18. ISSN 0248-4900. Available from: https://dx.doi.org/10.1111/boc.201500047.

@article{1318390,
   author = {Slaninová, Iva and LópezandSánchez, Noelia and Šebrlová, Kristýna and Vymazal, Ondřej and Frade, José María and Táborská, Eva},
   article_location = {Hoboken},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/boc.201500047},
   keywords = {Cell cycle; DNA dye; FACS; Macarpine; Time-lapse imaging},
   language = {eng},
   issn = {0248-4900},
   journal = {Biology of the Cell},
   title = {Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting},
   volume = {108},
   year = {2016}
}

TY  - JOUR
ID  - 1318390
AU  - Slaninová, Iva - López-Sánchez, Noelia - Šebrlová, Kristýna - Vymazal, Ondřej - Frade, José María - Táborská, Eva
PY  - 2016
TI  - Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting
JF  - Biology of the Cell
VL  - 108
IS  - 1
SP  - 1-18
EP  - 1-18
PB  - Wiley-Blackwell
SN  - 02484900
KW  - Cell cycle
KW  - DNA dye
KW  - FACS
KW  - Macarpine
KW  - Time-lapse imaging
N2  - Macarpine (MA) is a quaternary benzophenanthridine plant alkaloid isolated from Macleaya microcarpa or Stylophorum lasiocarpum. Benzophenanthridine alkaloids are interesting natural products that display antiproliferative, antimicrobial, antifungal and anti-inflammatory activities, and also fluorescence properties. In a previous study, we demonstrated that thanks to its ability to interact with DNA and its spectral properties MA could be used as a supravital DNA probe for fluorescence microscopy and flow cytometry including analyses of the cell cycle. In this study, we evaluated the suitability of MA as a DNA dye for time-lapse microscopy and flow-cytometric cell sorting.
ER  -

SLANINOVÁ, Iva, Noelia LÓPEZ-SÁNCHEZ, Kristýna ŠEBRLOVÁ, Ondřej VYMAZAL, José María FRADE and Eva TÁBORSKÁ. Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting. \textit{Biology of the Cell}. Hoboken: Wiley-Blackwell, 2016, vol.~108, No~1, p.~1-18. ISSN~0248-4900. Available from: https://dx.doi.org/10.1111/boc.201500047.

Detailed Information on Publication Record