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@article{1318410,
author = {Čejková, Darina and Strouhal, Michal and Norris, Steven J. and Weinstock, George M. and Šmajs, David},
article_location = {San Francisco},
article_number = {10},
doi = {http://dx.doi.org/10.1371/journal.pntd.0004110},
keywords = {PALLIDUM SUBSP PALLIDUM; COMPLETE GENOME SEQUENCE; BURROWS-WHEELER TRANSFORM; OUTER-MEMBRANE PROTEIN; ANTIGENIC VARIATION; ESCHERICHIA-COLI; MYCOBACTERIUM-TUBERCULOSIS; SSP-PALLIDUM; TPRK GENE; SYPHILIS TREPONEMES},
language = {eng},
issn = {1935-2735},
journal = {PLoS Neglected Tropical Diseases},
title = {A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions},
volume = {9},
year = {2015}
}
TY - JOUR
ID - 1318410
AU - Čejková, Darina - Strouhal, Michal - Norris, Steven J. - Weinstock, George M. - Šmajs, David
PY - 2015
TI - A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions
JF - PLoS Neglected Tropical Diseases
VL - 9
IS - 10
SP - 1-19
EP - 1-19
PB - Public Library of Science
SN - 19352735
KW - PALLIDUM SUBSP PALLIDUM
KW - COMPLETE GENOME SEQUENCE
KW - BURROWS-WHEELER TRANSFORM
KW - OUTER-MEMBRANE PROTEIN
KW - ANTIGENIC VARIATION
KW - ESCHERICHIA-COLI
KW - MYCOBACTERIUM-TUBERCULOSIS
KW - SSP-PALLIDUM
KW - TPRK GENE
KW - SYPHILIS TREPONEMES
N2 - BACKGROUND: Pathogenic uncultivable treponemes comprise human and animal pathogens including agents of syphilis, yaws, bejel, pinta, and venereal spirochetosis in rabbits and hares. A set of 10 treponemal genome sequences including those of 4 Treponema pallidum ssp. pallidum (TPA) strains (Nichols, DAL-1, Mexico A, SS14), 4 T. p. ssp. pertenue (TPE) strains (CDC-2, Gauthier, Samoa D, Fribourg-Blanc), 1 T. p. ssp. endemicum (TEN) strain (Bosnia A) and one strain (Cuniculi A) of Treponema paraluisleporidarum ecovar Cuniculus (TPLC) were examined with respect to the presence of nucleotide intrastrain heterogeneous sites. METHODOLOGY/PRINCIPAL FINDINGS: The number of identified intrastrain heterogeneous sites in individual genomes ranged between 0 and 7. Altogether, 23 intrastrain heterogeneous sites (in 17 genes) were found in 5 out of 10 investigated treponemal genomes including TPA strains Nichols (n = 5), DAL-1 (n = 4), and SS14 (n = 7), TPE strain Samoa D (n = 1), and TEN strain Bosnia A (n = 5). Although only one heterogeneous site was identified among 4 tested TPE strains, 16 such sites were identified among 4 TPA strains. Heterogeneous sites were mostly strain-specific and were identified in four tpr genes (tprC, GI, I, K), in genes involved in bacterial motility and chemotaxis (fliI, cheC-fliY), in genes involved in cell structure (murC), translation (prfA), general and DNA metabolism (putative SAM dependent methyltransferase, topA), and in seven hypothetical genes. CONCLUSIONS/SIGNIFICANCE: Heterogeneous sites likely represent both the selection of adaptive changes during infection of the host as well as an ongoing diversifying evolutionary process.
ER -
ČEJKOVÁ, Darina, Michal STROUHAL, Steven J. NORRIS, George M. WEINSTOCK and David ŠMAJS. A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions. \textit{PLoS Neglected Tropical Diseases}. San Francisco: Public Library of Science, 2015, vol.~9, No~10, p.~1-19. ISSN~1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0004110.
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