Isolation of human pathogen Escherichia albertii from faeces of seals (Leptonychos weddelli) in James Ross Island, Antarctica

SEDLÁČEK, Ivo, Linda GRILLOVÁ, Eva STAŇKOVÁ, Jitka ČERNOHLÁVKOVÁ and David ŠMAJS. Isolation of human pathogen Escherichia albertii from faeces of seals (Leptonychos weddelli) in James Ross Island, Antarctica. Czech polar Reports. 2013, vol. 3, No 2, p. 173-183. ISSN 1805-0689.

Basic information

• Original name: Isolation of human pathogen Escherichia albertii from faeces of seals (Leptonychos weddelli) in James Ross Island, Antarctica
• Authors: SEDLÁČEK, Ivo (203 Czech Republic, guarantor, belonging to the institution), Linda GRILLOVÁ (203 Czech Republic), Eva STAŇKOVÁ (203 Czech Republic), Jitka ČERNOHLÁVKOVÁ (203 Czech Republic) and David ŠMAJS (203 Czech Republic).
• Edition: Czech polar Reports, 2013, 1805-0689.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/13:00084963
• Organization unit: Faculty of Science
• Keywords in English: identification; bacteriocin; biotyping; ribotyping
• Tags: AKR, rivok

Abstract

A set of nine gram-negative fermenting rods biochemically identified as Escherichia coli was isolated from faeces of seals. These bacteria were characterized by phenotypic classification, 16S rDNA sequence analyses, automated ribotyping, study of whole-cell protein profiles by SDS-PAGE and finally by bacteriocin production. The results of our polyphasic taxonomic study supported the recognition of P4652, P4653 and P4740 isolates as true members of Escherichia albertii species – probably a major enteric human pathogen. To our best knowledge, this is the first evidence showing that E. albertii produces bacteriocin, and colicin D. Obtained data unambiguously showed inconvenience of commercial identification systems to distinguish both Escherichia species due to missing data of E. albertii in the commercial databases. The results of Escherichia isolates taxonomy suggest seals as a novel source of human and animal pathogen, E. albertii in the Antarctic region.

Links

• EE2.3.20.0183, research and development project: Name: Centrum experimentální biomedicíny
• LM2010009, research and development project: Name: Projekt CzechPolar - České polární stanice: Stavba a operační náklady

Investor: Ministry of Education, Youth and Sports of the CR