KASPAR, Petr, Martina ZIKOVA, Petr BARTUNEK, Jaroslav ŠTĚRBA, Hynek STRNAD, Leoš KŘEN and Radislav SEDLACEK. The Expression of c-Myb Correlates with the Levels of Rhabdomyosarcoma-specific Marker Myogenin. Scientific Reports. London: Nature Publishing Group, 2015, vol. 5, No 15090, p. 1-10. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/srep15090.
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Basic information
Original name The Expression of c-Myb Correlates with the Levels of Rhabdomyosarcoma-specific Marker Myogenin
Authors KASPAR, Petr (203 Czech Republic, guarantor), Martina ZIKOVA (203 Czech Republic), Petr BARTUNEK (203 Czech Republic), Jaroslav ŠTĚRBA (203 Czech Republic, belonging to the institution), Hynek STRNAD (203 Czech Republic), Leoš KŘEN (203 Czech Republic, belonging to the institution) and Radislav SEDLACEK (203 Czech Republic).
Edition Scientific Reports, London, Nature Publishing Group, 2015, 2045-2322.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 5.228
RIV identification code RIV/00216224:14110/15:00085064
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1038/srep15090
UT WoS 000362718600003
Keywords in English CELL-DIFFERENTIATION; PEDIATRIC RHABDOMYOSARCOMAS; GENE-EXPRESSION; DOWN-REGULATION; CANCER-CELLS; PROLIFERATION; MICRORNAS; SURVIVAL; LEUKEMIA; INVASION
Tags EL OK
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 3/12/2015 16:00.
Abstract
The transcription factor c-Myb is required for modulation of progenitor cells in several tissues, including skeletal muscle and its upregulation is observed in many human malignancies. Rhabdomyosarcomas (RMS) are a heterogeneous group of mesodermal tumors with features of developing skeletal muscle. Several miRNAs are downregulated in RMS, including miR-150, a negative regulator of c-Myb expression. Using the C2C12 myoblast cell line, a cellular model of skeletal muscle differentiation, we showed that miR-150 controls c-Myb expression mainly at the level of translation. We hypothesized that a similar mechanism of c-Myb regulation operates in RMS tumors. We examined expression of c-Myb by immunohistochemistry and revealed c-Myb positivity in alveolar and embryonal tumors, the two most common subgroups of RMS. Furthermore, we showed direct correlation between c-Myb production and myogenin expression. Interestingly, high myogenin levels indicate poor prognosis in RMS patients. c-Myb could, therefore, contribute to the tumor phenotype by executing its inhibitory role in skeletal muscle differentiation. We also showed that c-Myb protein is abundant in migratory C2C12 myoblasts and its ectopic expression potentiates cell motility. In summary, our results implicate that metastatic properties of some RMS subtypes might be linked to c-Myb function.
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