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@article{1320371,
author = {Svobodová, Markéta and Gumulec, Jaromír and Raudenská, Martina and Polanská, Hana and Holubová, Monika and Balvan, Jan and Hudcová, Kristýna and Knopfová, Lucia and Kizek, René and Adam, Vojtech and Babula, Petr and Masařík, Michal},
article_location = {Athens},
article_number = {4},
doi = {http://dx.doi.org/10.3892/ijo.2015.2883},
keywords = {breast cancer; zinc treatment; tumour size; metallothionein; glutathione},
language = {eng},
issn = {1019-6439},
journal = {International Journal of Oncology},
title = {Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment},
volume = {46},
year = {2015}
}
TY - JOUR
ID - 1320371
AU - Svobodová, Markéta - Gumulec, Jaromír - Raudenská, Martina - Polanská, Hana - Holubová, Monika - Balvan, Jan - Hudcová, Kristýna - Knopfová, Lucia - Kizek, René - Adam, Vojtech - Babula, Petr - Masařík, Michal
PY - 2015
TI - Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment
JF - International Journal of Oncology
VL - 46
IS - 4
SP - 1810-1818
EP - 1810-1818
PB - Spandidos Publications
SN - 10196439
KW - breast cancer
KW - zinc treatment
KW - tumour size
KW - metallothionein
KW - glutathione
N2 - Breast cancer patients negative for the nuclear oestrogen receptor alpha have a particularly poor prognosis. Therefore, the 4T1 cell line (considered as a triple-negative model) was chosen to induce malignancy in mice. The aim of the present study was to assess if zinc ions, provided in excess, may significantly modify the process of mammary oncogenesis. Zn(II) ions were chosen because of their documented antitumour effects. Zn(II) is also known to induce the expression of metallothioneins (MT) and glutathion (GSH). A total dose of zinc sulphate per one gram of mouse weight used in the experiment was 0.15 mg. We studied the expression of MT1 MT2, TP53 and MTF-1genes and also examined the effect of the tumour on antioxidant capacity. Tumour-free mice had significantly higher expression levels of the studied genes (P<0.003). Significant differences were also revealed in the gene expression between the tissues (P<0.001). The highest expression levels were observed in the liver. As compared to brain, lung and liver, significantly lower concentrations of MT protein were found in the primary tumour; an inverse trend was observed in the concentration of Zinc(II). In non-tumour mice, the amount of hepatic hydrosulphuryl groups significantly increased by the exposure to Zn(II), but the animals with tumour induction showed no similar trend. The primary tumour size of zinc-treated animals was 20% smaller (P=0.002); however, no significant effect on metastasis progression due to the zinc treatment was discovered. In conclusion, Zn(II) itself may mute the growth of primary breast tumours especially at their early stages.
ER -
SVOBODOVÁ, Markéta, Jaromír GUMULEC, Martina RAUDENSKÁ, Hana POLANSKÁ, Monika HOLUBOVÁ, Jan BALVAN, Kristýna HUDCOVÁ, Lucia KNOPFOVÁ, René KIZEK, Vojtech ADAM, Petr BABULA and Michal MASAŘÍK. Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment. \textit{International Journal of Oncology}. Athens: Spandidos Publications, 2015, vol.~46, No~4, p.~1810-1818. ISSN~1019-6439. Available from: https://dx.doi.org/10.3892/ijo.2015.2883.
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