Molecular response of 4T1-induced mouse mammary tumours and
healthy tissues to zinc treatment

J 2015

Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment

SVOBODOVÁ, Markéta, Jaromír GUMULEC, Martina RAUDENSKÁ, Hana POLANSKÁ, Monika HOLUBOVÁ et. al.

Basic information

Original name

Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment

Authors

SVOBODOVÁ, Markéta (203 Czech Republic, belonging to the institution), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Martina RAUDENSKÁ (203 Czech Republic, belonging to the institution), Hana POLANSKÁ (203 Czech Republic, belonging to the institution), Monika HOLUBOVÁ (203 Czech Republic, belonging to the institution), Jan BALVAN (203 Czech Republic, belonging to the institution), Kristýna HUDCOVÁ (203 Czech Republic, belonging to the institution), Lucia KNOPFOVÁ (203 Czech Republic), René KIZEK (203 Czech Republic), Vojtech ADAM (203 Czech Republic), Petr BABULA (203 Czech Republic, belonging to the institution) and Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Oncology, Athens, Spandidos Publications, 2015, 1019-6439

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Greece

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.018

RIV identification code

RIV/00216224:14110/15:00085106

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3892/ijo.2015.2883

UT WoS

000350616400042

Keywords in English

breast cancer; zinc treatment; tumour size; metallothionein; glutathione

Abstract

V originále

Breast cancer patients negative for the nuclear oestrogen receptor alpha have a particularly poor prognosis. Therefore, the 4T1 cell line (considered as a triple-negative model) was chosen to induce malignancy in mice. The aim of the present study was to assess if zinc ions, provided in excess, may significantly modify the process of mammary oncogenesis. Zn(II) ions were chosen because of their documented antitumour effects. Zn(II) is also known to induce the expression of metallothioneins (MT) and glutathion (GSH). A total dose of zinc sulphate per one gram of mouse weight used in the experiment was 0.15 mg. We studied the expression of MT1 MT2, TP53 and MTF-1genes and also examined the effect of the tumour on antioxidant capacity. Tumour-free mice had significantly higher expression levels of the studied genes (P<0.003). Significant differences were also revealed in the gene expression between the tissues (P<0.001). The highest expression levels were observed in the liver. As compared to brain, lung and liver, significantly lower concentrations of MT protein were found in the primary tumour; an inverse trend was observed in the concentration of Zinc(II). In non-tumour mice, the amount of hepatic hydrosulphuryl groups significantly increased by the exposure to Zn(II), but the animals with tumour induction showed no similar trend. The primary tumour size of zinc-treated animals was 20% smaller (P=0.002); however, no significant effect on metastasis progression due to the zinc treatment was discovered. In conclusion, Zn(II) itself may mute the growth of primary breast tumours especially at their early stages.

Links

MUNI/A/1003/2013, interní kód MU

Name: Molekulární patofyziologie vybraných komplexních nemocí

Investor: Masaryk University, Category A

ROZV/24/LF5/2014, interní kód MU

Name: Ovlivnění metabolismu nádorových buněk jako nový nástroj cílené terapie

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

SVOBODOVÁ, Markéta, Jaromír GUMULEC, Martina RAUDENSKÁ, Hana POLANSKÁ, Monika HOLUBOVÁ, Jan BALVAN, Kristýna HUDCOVÁ, Lucia KNOPFOVÁ, René KIZEK, Vojtech ADAM, Petr BABULA and Michal MASAŘÍK. Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment. International Journal of Oncology. Athens: Spandidos Publications, 2015, vol. 46, No 4, p. 1810-1818. ISSN 1019-6439. Available from: https://dx.doi.org/10.3892/ijo.2015.2883.

@article{1320371,
   author = {Svobodová, Markéta and Gumulec, Jaromír and Raudenská, Martina and Polanská, Hana and Holubová, Monika and Balvan, Jan and Hudcová, Kristýna and Knopfová, Lucia and Kizek, René and Adam, Vojtech and Babula, Petr and Masařík, Michal},
   article_location = {Athens},
   article_number = {4},
   doi = {http://dx.doi.org/10.3892/ijo.2015.2883},
   keywords = {breast cancer; zinc treatment; tumour size; metallothionein; glutathione},
   language = {eng},
   issn = {1019-6439},
   journal = {International Journal of Oncology},
   title = {Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment},
   volume = {46},
   year = {2015}
}

TY  - JOUR
ID  - 1320371
AU  - Svobodová, Markéta - Gumulec, Jaromír - Raudenská, Martina - Polanská, Hana - Holubová, Monika - Balvan, Jan - Hudcová, Kristýna - Knopfová, Lucia - Kizek, René - Adam, Vojtech - Babula, Petr - Masařík, Michal
PY  - 2015
TI  - Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment
JF  - International Journal of Oncology
VL  - 46
IS  - 4
SP  - 1810-1818
EP  - 1810-1818
PB  - Spandidos Publications
SN  - 10196439
KW  - breast cancer
KW  - zinc treatment
KW  - tumour size
KW  - metallothionein
KW  - glutathione
N2  - Breast cancer patients negative for the nuclear oestrogen receptor alpha have a particularly poor prognosis. Therefore, the 4T1 cell line (considered as a triple-negative model) was chosen to induce malignancy in mice. The aim of the present study was to assess if zinc ions, provided in excess, may significantly modify the process of mammary oncogenesis. Zn(II) ions were chosen because of their documented antitumour effects. Zn(II) is also known to induce the expression of metallothioneins (MT) and glutathion (GSH). A total dose of zinc sulphate per one gram of mouse weight used in the experiment was 0.15 mg. We studied the expression of MT1 MT2, TP53 and MTF-1genes and also examined the effect of the tumour on antioxidant capacity. Tumour-free mice had significantly higher expression levels of the studied genes (P<0.003). Significant differences were also revealed in the gene expression between the tissues (P<0.001). The highest expression levels were observed in the liver. As compared to brain, lung and liver, significantly lower concentrations of MT protein were found in the primary tumour; an inverse trend was observed in the concentration of Zinc(II). In non-tumour mice, the amount of hepatic hydrosulphuryl groups significantly increased by the exposure to Zn(II), but the animals with tumour induction showed no similar trend. The primary tumour size of zinc-treated animals was 20% smaller (P=0.002); however, no significant effect on metastasis progression due to the zinc treatment was discovered. In conclusion, Zn(II) itself may mute the growth of primary breast tumours especially at their early stages.
ER  -

SVOBODOVÁ, Markéta, Jaromír GUMULEC, Martina RAUDENSKÁ, Hana POLANSKÁ, Monika HOLUBOVÁ, Jan BALVAN, Kristýna HUDCOVÁ, Lucia KNOPFOVÁ, René KIZEK, Vojtech ADAM, Petr BABULA and Michal MASAŘÍK. Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment. \textit{International Journal of Oncology}. Athens: Spandidos Publications, 2015, vol.~46, No~4, p.~1810-1818. ISSN~1019-6439. Available from: https://dx.doi.org/10.3892/ijo.2015.2883.

Detailed Information on Publication Record