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@article{1320569, author = {Luker, K.E. and Pata, P. and Shemiakina, I.I. and Pereverzeva, Alina and Stacer, A.C. and Shcherbo, D.S. and Pletnev, V.Z. and Skolnaja, M. and Lukyanov, K.A. and Luker, G.D. and Pata, I. and Chudakov, Dmitriy}, article_location = {LONDON}, article_number = {June}, doi = {http://dx.doi.org/10.1038/srep10332}, keywords = {far-red fuorescent protein; comparative study; body imaging}, language = {eng}, issn = {2045-2322}, journal = {Scientific Reports}, title = {Comparative study reveals better far-red fluorescent protein for whole body imaging}, url = {http://www.nature.com/articles/srep10332}, volume = {5}, year = {2015} }
TY - JOUR ID - 1320569 AU - Luker, K.E. - Pata, P. - Shemiakina, I.I. - Pereverzeva, Alina - Stacer, A.C. - Shcherbo, D.S. - Pletnev, V.Z. - Skolnaja, M. - Lukyanov, K.A. - Luker, G.D. - Pata, I. - Chudakov, Dmitriy PY - 2015 TI - Comparative study reveals better far-red fluorescent protein for whole body imaging JF - Scientific Reports VL - 5 IS - June SP - "nestránkováno" EP - "nestránkováno" PB - NATURE PUBLISHING GROUP SN - 20452322 KW - far-red fuorescent protein KW - comparative study KW - body imaging UR - http://www.nature.com/articles/srep10332 L2 - http://www.nature.com/articles/srep10332 N2 - Genetically encoded far-red and near-infrared fluorescent proteins enable efficient imaging in studies of tumorigenesis, embryogenesis, and inflammation in model animals. Here we report comparative testing of available GFP-like far-red fluorescent proteins along with a modified protein, named Katushka2S, and near-infrared bacterial phytochrome-based markers. We compare fluorescence signal and signal-to-noise ratio at various excitation wavelength and emission filter combinations using transiently transfected cell implants in mice, providing a basis for rational choice of optimal marker(s) for in vivo imaging studies. We demonstrate that the signals of various far-red fluorescent proteins can be spectrally unmixed based on different signal-to-noise ratios in different channels, providing the straightforward possibility of multiplexed imaging with standard equipment. Katushka2S produced the brightest and fastest maturing fluorescence in all experimental setups. At the same time, signal-to-noise ratios for Katushka2S and near-infrared bacterial phytochrome, iRFP720 were comparable in their optimal channels. Distinct spectral and genetic characteristics suggest this pair of a far-red and a near-infrared fluorescent protein as an optimal combination for dual color, whole body imaging studies in model animals. ER -
LUKER, K.E., P. PATA, I.I. SHEMIAKINA, Alina PEREVERZEVA, A.C. STACER, D.S. SHCHERBO, V.Z. PLETNEV, M. SKOLNAJA, K.A. LUKYANOV, G.D. LUKER, I. PATA a Dmitriy CHUDAKOV. Comparative study reveals better far-red fluorescent protein for whole body imaging. \textit{Scientific Reports}. LONDON: NATURE PUBLISHING GROUP, roč.~5, June, s.~''nestránkováno'', 9 s. ISSN~2045-2322. doi:10.1038/srep10332. 2015.
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