LUKER, K.E., P. PATA, I.I. SHEMIAKINA, Alina PEREVERZEVA, A.C. STACER, D.S. SHCHERBO, V.Z. PLETNEV, M. SKOLNAJA, K.A. LUKYANOV, G.D. LUKER, I. PATA and Dmitriy CHUDAKOV. Comparative study reveals better far-red fluorescent protein for whole body imaging (Comparative study reveals better far-red fluorescent protein for whole body imaging.). Scientific Reports. LONDON: NATURE PUBLISHING GROUP, 2015, vol. 5, June, p. "nestránkováno", 9 pp. ISSN 2045-2322. doi:10.1038/srep10332.
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Basic information
Original name Comparative study reveals better far-red fluorescent protein for whole body imaging
Authors LUKER, K.E. (840 United States of America), P. PATA (233 Estonia), I.I. SHEMIAKINA (643 Russian Federation), Alina PEREVERZEVA (643 Russian Federation), A.C. STACER (840 United States of America), D.S. SHCHERBO (643 Russian Federation), V.Z. PLETNEV (643 Russian Federation), M. SKOLNAJA (643 Russian Federation), K.A. LUKYANOV (643 Russian Federation), G.D. LUKER (840 United States of America), I. PATA (643 Russian Federation) and Dmitriy CHUDAKOV (643 Russian Federation, guarantor, belonging to the institution).
Edition Scientific Reports, LONDON, NATURE PUBLISHING GROUP, 2015, 2045-2322.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30200 3.2 Clinical medicine
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.228
RIV identification code RIV/00216224:14740/15:00085159
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1038/srep10332
UT WoS 000355608400001
Keywords in English far-red fuorescent protein; comparative study; body imaging
Tags OA, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Eva Špillingová, učo 110713. Changed: 4. 4. 2016 16:02.
Abstract
Genetically encoded far-red and near-infrared fluorescent proteins enable efficient imaging in studies of tumorigenesis, embryogenesis, and inflammation in model animals. Here we report comparative testing of available GFP-like far-red fluorescent proteins along with a modified protein, named Katushka2S, and near-infrared bacterial phytochrome-based markers. We compare fluorescence signal and signal-to-noise ratio at various excitation wavelength and emission filter combinations using transiently transfected cell implants in mice, providing a basis for rational choice of optimal marker(s) for in vivo imaging studies. We demonstrate that the signals of various far-red fluorescent proteins can be spectrally unmixed based on different signal-to-noise ratios in different channels, providing the straightforward possibility of multiplexed imaging with standard equipment. Katushka2S produced the brightest and fastest maturing fluorescence in all experimental setups. At the same time, signal-to-noise ratios for Katushka2S and near-infrared bacterial phytochrome, iRFP720 were comparable in their optimal channels. Distinct spectral and genetic characteristics suggest this pair of a far-red and a near-infrared fluorescent protein as an optimal combination for dual color, whole body imaging studies in model animals.
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