J 2016

Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3K/Akt and MAPK/Erk Activity in Mouse Embryonic Stem Cells

KUČERA, Jan, Lucia BINÓ, Kateřina ŠTEFKOVÁ, Josef JAROŠ, Ondřej VAŠÍČEK et. al.

Basic information

Original name

Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3K/Akt and MAPK/Erk Activity in Mouse Embryonic Stem Cells

Authors

KUČERA, Jan (203 Czech Republic, belonging to the institution), Lucia BINÓ (703 Slovakia, belonging to the institution), Kateřina ŠTEFKOVÁ (203 Czech Republic, belonging to the institution), Josef JAROŠ (203 Czech Republic, belonging to the institution), Ondřej VAŠÍČEK (203 Czech Republic), Josef VEČEŘA (203 Czech Republic, belonging to the institution), Lukáš KUBALA (203 Czech Republic) and Jiří PACHERNÍK (203 Czech Republic, belonging to the institution)

Edition

Oxidative Medicine and Cellular Longevity, Hindawi Publishing Corporation, 2016, 1942-0900

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.593

RIV identification code

RIV/00216224:14310/16:00089209

Organization unit

Faculty of Science

DOI

UT WoS

000369250100001

Keywords in English

NADPH OXIDASE INHIBITOR; SMOOTH-MUSCLE-CELLS; WNT-BETA-CATENIN; REACTIVE OXYGEN; SELF-RENEWAL; CARDIOMYOCYTE DIFFERENTIATION; INTRACELLULAR SUPEROXIDE; ENDOTHELIAL-CELLS; REDOX-REGULATION; FREE-RADICALS

Tags

Tags

International impact, Reviewed
Změněno: 30/3/2017 10:12, Ing. Andrea Mikešková

Abstract

V originále

Reactive oxygen species (ROS) are important regulators of cellular functions. In embryonic stem cells, ROS are suggested to influence differentiation status. Regulated ROS formation is catalyzed primarily by NADPH-dependent oxidases (NOXs). Apocynin and diphenyleneiodonium are frequently used inhibitors of NOXs; however, both exhibit uncharacterized effects not related to NOXs inhibition. Interestingly, in our model of mouse embryonic stem cells we demonstrate low expression of NOXs. Therefore we aimed to clarify potential side effects of these drugs. Both apocynin and diphenyleneiodonium impaired proliferation of cells. Surprisingly, we observed prooxidant activity of these drugs determined by hydroethidine. Further, we revealed that apocynin inhibits PI3K/Akt pathway with its downstream transcriptional factor Nanog. Opposite to this, apocynin augmented activity of canonical Wnt signaling. On the contrary, diphenyleneiodonium activated both PI3K/Akt and Erk signaling pathways without affecting Wnt. Our data indicates limits and possible unexpected interactions of NOXs inhibitors with intracellular signaling pathways.

Links

ED3.2.00/08.0144, research and development project
Name: CERIT Scientific Cloud
EE2.3.20.0180, research and development project
Name: Spolupráce mezi Masarykovou univerzitou a Karolinska Institutet, Stockholm na poli biomedicíny
EE2.3.30.0009, research and development project
Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci
LM2010005, research and development project
Name: Velká infrastruktura CESNET (Acronym: VI CESNET)
Investor: Ministry of Education, Youth and Sports of the CR
MSM0021622430, plan (intention)
Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie
Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
MUNI/A/1558/2014, interní kód MU
Name: Zdroje pro tkáňové inženýrství 5 (Acronym: TissueEng5)
Investor: Masaryk University, Category A