KUČERA, Jan, Lucia BINÓ, Kateřina ŠTEFKOVÁ, Josef JAROŠ, Ondřej VAŠÍČEK, Josef VEČEŘA, Lukáš KUBALA and Jiří PACHERNÍK. Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3K/Akt and MAPK/Erk Activity in Mouse Embryonic Stem Cells. Oxidative Medicine and Cellular Longevity. Hindawi Publishing Corporation, 2016, vol. 2016, No 7409196, p. 1-14. ISSN 1942-0900. Available from: https://dx.doi.org/10.1155/2016/7409196. |
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@article{1320663, author = {Kučera, Jan and Binó, Lucia and Štefková, Kateřina and Jaroš, Josef and Vašíček, Ondřej and Večeřa, Josef and Kubala, Lukáš and Pacherník, Jiří}, article_number = {7409196}, doi = {http://dx.doi.org/10.1155/2016/7409196}, keywords = {NADPH OXIDASE INHIBITOR; SMOOTH-MUSCLE-CELLS; WNT-BETA-CATENIN; REACTIVE OXYGEN; SELF-RENEWAL; CARDIOMYOCYTE DIFFERENTIATION; INTRACELLULAR SUPEROXIDE; ENDOTHELIAL-CELLS; REDOX-REGULATION; FREE-RADICALS}, language = {eng}, issn = {1942-0900}, journal = {Oxidative Medicine and Cellular Longevity}, title = {Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3K/Akt and MAPK/Erk Activity in Mouse Embryonic Stem Cells}, volume = {2016}, year = {2016} }
TY - JOUR ID - 1320663 AU - Kučera, Jan - Binó, Lucia - Štefková, Kateřina - Jaroš, Josef - Vašíček, Ondřej - Večeřa, Josef - Kubala, Lukáš - Pacherník, Jiří PY - 2016 TI - Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3K/Akt and MAPK/Erk Activity in Mouse Embryonic Stem Cells JF - Oxidative Medicine and Cellular Longevity VL - 2016 IS - 7409196 SP - 1-14 EP - 1-14 PB - Hindawi Publishing Corporation SN - 19420900 KW - NADPH OXIDASE INHIBITOR KW - SMOOTH-MUSCLE-CELLS KW - WNT-BETA-CATENIN KW - REACTIVE OXYGEN KW - SELF-RENEWAL KW - CARDIOMYOCYTE DIFFERENTIATION KW - INTRACELLULAR SUPEROXIDE KW - ENDOTHELIAL-CELLS KW - REDOX-REGULATION KW - FREE-RADICALS N2 - Reactive oxygen species (ROS) are important regulators of cellular functions. In embryonic stem cells, ROS are suggested to influence differentiation status. Regulated ROS formation is catalyzed primarily by NADPH-dependent oxidases (NOXs). Apocynin and diphenyleneiodonium are frequently used inhibitors of NOXs; however, both exhibit uncharacterized effects not related to NOXs inhibition. Interestingly, in our model of mouse embryonic stem cells we demonstrate low expression of NOXs. Therefore we aimed to clarify potential side effects of these drugs. Both apocynin and diphenyleneiodonium impaired proliferation of cells. Surprisingly, we observed prooxidant activity of these drugs determined by hydroethidine. Further, we revealed that apocynin inhibits PI3K/Akt pathway with its downstream transcriptional factor Nanog. Opposite to this, apocynin augmented activity of canonical Wnt signaling. On the contrary, diphenyleneiodonium activated both PI3K/Akt and Erk signaling pathways without affecting Wnt. Our data indicates limits and possible unexpected interactions of NOXs inhibitors with intracellular signaling pathways. ER -
KUČERA, Jan, Lucia BINÓ, Kateřina ŠTEFKOVÁ, Josef JAROŠ, Ondřej VAŠÍČEK, Josef VEČEŘA, Lukáš KUBALA and Jiří PACHERNÍK. Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3K/Akt and MAPK/Erk Activity in Mouse Embryonic Stem Cells. \textit{Oxidative Medicine and Cellular Longevity}. Hindawi Publishing Corporation, 2016, vol.~2016, No~7409196, p.~1-14. ISSN~1942-0900. Available from: https://dx.doi.org/10.1155/2016/7409196.
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