Nanostructured gold deposited in gelatin template applied for
electrochemical assay of glucose in serum

J 2016

Nanostructured gold deposited in gelatin template applied for electrochemical assay of glucose in serum

JUŘÍK, Tomáš, Pavel PODEŠVA, Zdeněk FARKA, David KOVÁŘ, Petr SKLÁDAL et. al.

Basic information

Original name

Nanostructured gold deposited in gelatin template applied for electrochemical assay of glucose in serum

Authors

JUŘÍK, Tomáš (203 Czech Republic, belonging to the institution), Pavel PODEŠVA (203 Czech Republic), Zdeněk FARKA (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution) and František FORET (203 Czech Republic, belonging to the institution)

Edition

Electrochimica Acta, Oxford, Pergamon-Elsevier, 2016, 0013-4686

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.798

RIV identification code

RIV/00216224:14740/16:00089213

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.electacta.2015.12.009

UT WoS

000370986500032

Keywords in English

Gold nanostructures; Gelatin template; Glucose electrooxidation; Non-enzymatic sensor; Blood analysis

Abstract

V originále

Gelatin-templated gold nanostructures were fabricated in order to improve the sensitivity of non-enzymatic determination of glucose. The three-dimensional cavities were created by crosslinking of gelatin layer on the electrode and the specific saw tooth-like morphology of surface was formed by electrodeposition of gold through the cavities. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used for the investigation of nanostructure growth and characterization of prepared surfaces. Glucose was detected by a direct electrochemical oxidation during cyclic voltammetry in alkaline solution with excellent sensitivity of 1165 uA mM-1 cm-2, wide linear range from 32 uM to 10 mM and limit of detection (LOD) of 90 uM. During amperometric measurements LOD of 1.5 uM was achieved. The sensor was able to detect real concentrations of glucose in deproteinized human serum with negligible interference of oxidizable blood components. Great correlation with results obtained from reference methods (commercial glucometer and standard kit for photometric glucose detection) was demonstrated; the determination of glucose in serum was performed with relative standard deviation of 4%. The sensor exhibits a strong potential as a rapid and sensitive non-enzymatic approach in future applications for clinical analyses.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

JUŘÍK, Tomáš, Pavel PODEŠVA, Zdeněk FARKA, David KOVÁŘ, Petr SKLÁDAL and František FORET. Nanostructured gold deposited in gelatin template applied for electrochemical assay of glucose in serum. Electrochimica Acta. Oxford: Pergamon-Elsevier, 2016, vol. 188, January, p. 277-285. ISSN 0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2015.12.009.

@article{1321442,
   author = {Juřík, Tomáš and Podešva, Pavel and Farka, Zdeněk and Kovář, David and Skládal, Petr and Foret, František},
   article_location = {Oxford},
   article_number = {January},
   doi = {http://dx.doi.org/10.1016/j.electacta.2015.12.009},
   keywords = {Gold nanostructures; Gelatin template; Glucose electrooxidation; Non-enzymatic sensor; Blood analysis},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Nanostructured gold deposited in gelatin template applied for electrochemical assay of glucose in serum},
   url = {http://www.sciencedirect.com/science/article/pii/S0013468615309336},
   volume = {188},
   year = {2016}
}

TY  - JOUR
ID  - 1321442
AU  - Juřík, Tomáš - Podešva, Pavel - Farka, Zdeněk - Kovář, David - Skládal, Petr - Foret, František
PY  - 2016
TI  - Nanostructured gold deposited in gelatin template applied for electrochemical assay of glucose in serum
JF  - Electrochimica Acta
VL  - 188
IS  - January
SP  - 277-285
EP  - 277-285
PB  - Pergamon-Elsevier
SN  - 00134686
KW  - Gold nanostructures
KW  - Gelatin template
KW  - Glucose electrooxidation
KW  - Non-enzymatic sensor
KW  - Blood analysis
UR  - http://www.sciencedirect.com/science/article/pii/S0013468615309336
L2  - http://www.sciencedirect.com/science/article/pii/S0013468615309336
N2  - Gelatin-templated gold nanostructures were fabricated in order to improve the sensitivity of non-enzymatic determination of glucose. The three-dimensional cavities were created by crosslinking of gelatin layer on the electrode and the specific saw tooth-like morphology of surface was formed by electrodeposition of gold through the cavities. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used for the investigation of nanostructure growth and characterization of prepared surfaces. Glucose was detected by a direct electrochemical oxidation during cyclic voltammetry in alkaline solution with excellent sensitivity of 1165 uA mM-1 cm-2, wide linear range from 32 uM to 10 mM and limit of detection (LOD) of 90 uM. During amperometric measurements LOD of 1.5 uM was achieved. The sensor was able to detect real concentrations of glucose in deproteinized human serum with negligible interference of oxidizable blood components. Great correlation with results obtained from reference methods (commercial glucometer and standard kit for photometric glucose detection) was demonstrated; the determination of glucose in serum was performed with relative standard deviation of 4%. The sensor exhibits a strong potential as a rapid and sensitive non-enzymatic approach in future applications for clinical analyses.
ER  -

JUŘÍK, Tomáš, Pavel PODEŠVA, Zdeněk FARKA, David KOVÁŘ, Petr SKLÁDAL and František FORET. Nanostructured gold deposited in gelatin template applied for electrochemical assay of glucose in serum. \textit{Electrochimica Acta}. Oxford: Pergamon-Elsevier, 2016, vol.~188, January, p.~277-285. ISSN~0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2015.12.009.

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