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KYSEĽ, Peter, Gabriel DEMO, Stanislav HUSZÁR, Katarina MIKUŠOVÁ a Michaela WIMMEROVÁ. EXPRESSION AND CRYSTALLIZATION OF MYCOBACTERIAL GALACTOFURANOSYL TRANSFERASE GLFT1. In XVII. setkání biochemiků a molekulárních biologů. 2015. ISBN 978-80-210-8015-7.

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Základní údaje
Originální název	EXPRESSION AND CRYSTALLIZATION OF MYCOBACTERIAL GALACTOFURANOSYL TRANSFERASE GLFT1
Autoři	KYSEĽ, Peter (703 Slovensko, domácí), Gabriel DEMO (703 Slovensko, domácí), Stanislav HUSZÁR (703 Slovensko), Katarina MIKUŠOVÁ (703 Slovensko) a Michaela WIMMEROVÁ (203 Česká republika).
Vydání	XVII. setkání biochemiků a molekulárních biologů, 2015.

Další údaje
Originální jazyk	angličtina
Typ výsledku	Konferenční abstrakt
Obor	10600 1.6 Biological sciences
Stát vydavatele	Česká republika
Utajení	není předmětem státního či obchodního tajemství
WWW	URL
Kód RIV	RIV/00216224:14310/15:00085288
Organizační jednotka	Přírodovědecká fakulta
ISBN	978-80-210-8015-7
Klíčová slova anglicky	mycobacterium; glycosyltransferase; purification; detergent;crystallography
Štítky	AKR
Změnil	Změnila: Ing. Andrea Mikešková, učo 137293. Změněno: 28. 4. 2016 15:12.

Anotace
A number of glycosyltransferases is involved in the mycobacterial cell wall biosynthesis. The membrane associated galactofuranosyltransferase GlfT1 adds the first two galactofuranosyl residues of the galactan component of the arabinogalactan. Yet the esentiality of the GlfT1 for the proliferation of persistent mycobacterial pathogens provided a good reason to compose and optimise a combination of different biochemical approaches for purification and crystallization of this enzyme. We developed a multistep purification procedure to counter the pitfalls of proteolysis, chaperone contamination, precipitation and polydispersity. Two different conditions were identified to faciliate the crystallization of the fusion protein. X-ray diffraction data to 2.1 A were obtained. Solving of GlfT1 3D structure will eventually deliver structural insights into the first steps of galactan biosynthesis.

Anotace

A number of glycosyltransferases is involved in the mycobacterial cell wall biosynthesis. The membrane associated galactofuranosyltransferase GlfT1 adds the first two galactofuranosyl residues of the galactan component of the arabinogalactan. Yet the esentiality of the GlfT1 for the proliferation of persistent mycobacterial pathogens provided a good reason to compose and optimise a combination of different biochemical approaches for purification and crystallization of this enzyme. We developed a multistep purification procedure to counter the pitfalls of proteolysis, chaperone contamination, precipitation and polydispersity. Two different conditions were identified to faciliate the crystallization of the fusion protein. X-ray diffraction data to 2.1 A were obtained. Solving of GlfT1 3D structure will eventually deliver structural insights into the first steps of galactan biosynthesis.

Návaznosti
ED1.1.00/02.0068, projekt VaV	Název: CEITEC - central european institute of technology