J 2015

Antineoplastic effects of Chlorella pyrenoidosa in the breast cancer model

KUBATKA, Peter, Andrea KAPINOVÁ, Peter KRUŽLIAK, Martin KELLO, Desanka VÝBOHOVÁ et. al.

Basic information

Original name

Antineoplastic effects of Chlorella pyrenoidosa in the breast cancer model

Authors

KUBATKA, Peter (703 Slovakia), Andrea KAPINOVÁ (703 Slovakia), Peter KRUŽLIAK (203 Czech Republic, guarantor), Martin KELLO (703 Slovakia), Desanka VÝBOHOVÁ (703 Slovakia), Karol KAJO (703 Slovakia), Miroslav NOVÁK (203 Czech Republic, belonging to the institution), Martina CHRIPKOVÁ (703 Slovakia), Marián ADAMKOV (703 Slovakia), Martin PÉČ (703 Slovakia), Ján MOJŽIŠ (703 Slovakia), Bianka BOJKOVÁ (703 Slovakia), Monika KASSAYOVÁ (703 Slovakia), Nadežda STOLLÁROVÁ (703 Slovakia) and Dušan DOBROTA (703 Slovakia)

Edition

Nutrition, New York, Elsevier Science INC, 2015, 0899-9007

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30304 Public and environmental health

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.839

RIV identification code

RIV/00216224:14110/15:00085443

Organization unit

Faculty of Medicine

DOI

UT WoS

000351975200003

Keywords in English

Mammary carcinogenesis; Rat; Chlorella; Angiogenesis; Apoptosis; Cell proliferation; MCF-7

Tags

Tags

International impact, Reviewed
Změněno: 29/12/2015 14:35, Ing. Mgr. Věra Pospíšilíková

Abstract

V originále

Objectives: There has been considerable interest in both clinical and preclinical research about the role of phytochemicals in the reduction of risk for cancer in humans. The aim of this study was to determine the antineoplastic effects of Chlorella pyrenoidosa in experimental breast cancer in vivo and in vitro. Methods: In this experiment, the antineoplastic effects of Chlorella pyrenoidosa in the chemoprevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis in female rats were evaluated. Chlorella powder was administered through diet at concentrations of 0.3% and 3%. The experiment was terminated 14 wk after carcinogen administration. At autopsy, mammary tumors were removed and prepared for histopathological and immunohistochemical analysis. In vitro cytotoxicity assay, parameters of apoptosis, and proliferation after chlorella treatment in human breast adenocarcinoma (MCF-7) cells were carried out. Results: Basic parameters of experimental carcinogenesis, mechanism of action (biomarkers of apoptosis, proliferation, and angiogenesis), chosen metabolic variables, and side effects after long-term chlorella treatment in animals were assessed. Chlorella at higher concentration suppressed tumor frequency by 61% (P < 0.02) and lengthened tumor latency by 12.5 d (P < 0.02) in comparison with the controls. Immunohistochemical analysis of rat tumor cells showed caspase-7 expression increase by 73.5% (P < 0.001) and vascular endothelial growth factor receptor-2 expression decrease by 19% (P = 0.07) after chlorella treatment. In a parallel in vitro study, chlorella significantly decreased survival of MCF-7 cells in a dose-dependent manner. In chlorella-treated MCF-7 cells, a significant increase in cells having sub-G(0)/G(1) DNA content and significant increase of early apoptotic and late apoptotic/necrotic cells after annexin V/PI staining assay were found. Decreases in mitochondrial membrane potential and increasing reactive oxygen species generation were observed in the chlorella-treated MCF-7 cells. Conclusions: This study is the first report on the antineoplastic effects of Chlorella pyrenoidosa in experimental breast cancer in vivo and in vitro. (C) 2015 Elsevier Inc. All rights reserved.