STEJSKAL, Stanislav, Karel ŠTĚPKA, Lenka TESAŘOVÁ, Karel STEJSKAL, Martina MATĚJKOVÁ, Pavel ŠIMARA, Zbyněk ZDRÁHAL and Irena KRONTORÁD KOUTNÁ. Cell cycle-dependent changes in H3K56ac in human cells. Cell Cycle. Philadelphia: Taylor&Francis Inc, vol. 14, No 24, p. 3851-3863. ISSN 1538-4101. doi:10.1080/15384101.2015.1106760. 2015.
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Basic information
Original name Cell cycle-dependent changes in H3K56ac in human cells.
Authors STEJSKAL, Stanislav (203 Czech Republic, belonging to the institution), Karel ŠTĚPKA (203 Czech Republic, belonging to the institution), Lenka TESAŘOVÁ (203 Czech Republic, belonging to the institution), Karel STEJSKAL (203 Czech Republic, belonging to the institution), Martina MATĚJKOVÁ (203 Czech Republic, belonging to the institution), Pavel ŠIMARA (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution) and Irena KRONTORÁD KOUTNÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition Cell Cycle, Philadelphia, Taylor&Francis Inc, 2015, 1538-4101.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10601 Cell biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW Full Text
Impact factor Impact factor: 3.952
RIV identification code RIV/00216224:14330/15:00081398
Organization unit Faculty of Informatics
Doi http://dx.doi.org/10.1080/15384101.2015.1106760
UT WoS 000367063200017
Keywords in English Cell cycle; Chromatin; DNA replication; H3K56ac; Mammalian cells; Nucleosome
Tags cbia-web, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 16/12/2019 16:41.
Abstract
The incorporation of histone H3 with an acetylated lysine 56 (H3K56ac) into the nucleosome is important for chromatin remodeling and serves as a marker of new nucleosomes during DNA replication and repair in yeast. However, in human cells, the level of H3K56ac is greatly reduced, and its role during the cell cycle is controversial. Our aim was to determine the potential of H3K56ac to regulate cell cycle progression in different human cell lines. A significant increase in the number of H3K56ac foci, but not in H3K56ac protein levels, was observed during the S and G2 phases in cancer cell lines, but was not observed in embryonic stem cell lines. Despite this increase, the H3K56ac signal was not present in late replication chromatin, and H3K56ac protein levels did not decrease after the inhibition of DNA replication. H3K56ac was not tightly associated with the chromatin and was primarily localized to active chromatin regions. Our results support the role of H3K56ac in transcriptionally active chromatin areas but do not confirm H3K56ac as a marker of newly synthetized nucleosomes in DNA replication.
Links
CZ.1.07/2.3.00/30.0030, interní kód MUName: Rozvoj lidských zdrojů pro oblast buněčné biologie
Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
GBP206/12/G151, research and development projectName: Centrum nových přístupů k bioanalýze a molekulární diagnostice
GBP302/12/G157, research and development projectName: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii
Investor: Czech Science Foundation
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