Cell cycle-dependent changes in H3K56ac in human cells.

J 2015

Cell cycle-dependent changes in H3K56ac in human cells.

STEJSKAL, Stanislav, Karel ŠTĚPKA, Lenka TESAŘOVÁ, Karel STEJSKAL, Martina MATĚJKOVÁ et. al.

Basic information

Original name

Cell cycle-dependent changes in H3K56ac in human cells.

Authors

STEJSKAL, Stanislav (203 Czech Republic, belonging to the institution), Karel ŠTĚPKA (203 Czech Republic, belonging to the institution), Lenka TESAŘOVÁ (203 Czech Republic, belonging to the institution), Karel STEJSKAL (203 Czech Republic, belonging to the institution), Martina MATĚJKOVÁ (203 Czech Republic, belonging to the institution), Pavel ŠIMARA (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution) and Irena KRONTORÁD KOUTNÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Cell Cycle, Philadelphia, Taylor&Francis Inc, 2015, 1538-4101

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 3.952

RIV identification code

RIV/00216224:14330/15:00081398

Organization unit

Faculty of Informatics

DOI

http://dx.doi.org/10.1080/15384101.2015.1106760

UT WoS

000367063200017

Keywords in English

Cell cycle; Chromatin; DNA replication; H3K56ac; Mammalian cells; Nucleosome

Abstract

V originále

The incorporation of histone H3 with an acetylated lysine 56 (H3K56ac) into the nucleosome is important for chromatin remodeling and serves as a marker of new nucleosomes during DNA replication and repair in yeast. However, in human cells, the level of H3K56ac is greatly reduced, and its role during the cell cycle is controversial. Our aim was to determine the potential of H3K56ac to regulate cell cycle progression in different human cell lines. A significant increase in the number of H3K56ac foci, but not in H3K56ac protein levels, was observed during the S and G2 phases in cancer cell lines, but was not observed in embryonic stem cell lines. Despite this increase, the H3K56ac signal was not present in late replication chromatin, and H3K56ac protein levels did not decrease after the inhibition of DNA replication. H3K56ac was not tightly associated with the chromatin and was primarily localized to active chromatin regions. Our results support the role of H3K56ac in transcriptionally active chromatin areas but do not confirm H3K56ac as a marker of newly synthetized nucleosomes in DNA replication.

Links

CZ.1.07/2.3.00/30.0030, interní kód MU

Name: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GBP206/12/G151, research and development project

Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

Citovat

STEJSKAL, Stanislav, Karel ŠTĚPKA, Lenka TESAŘOVÁ, Karel STEJSKAL, Martina MATĚJKOVÁ, Pavel ŠIMARA, Zbyněk ZDRÁHAL and Irena KRONTORÁD KOUTNÁ. Cell cycle-dependent changes in H3K56ac in human cells. Cell Cycle. Philadelphia: Taylor&Francis Inc, 2015, vol. 14, No 24, p. 3851-3863. ISSN 1538-4101. Available from: https://dx.doi.org/10.1080/15384101.2015.1106760.

@article{1322928,
   author = {Stejskal, Stanislav and Štěpka, Karel and Tesařová, Lenka and Stejskal, Karel and Matějková, Martina and Šimara, Pavel and Zdráhal, Zbyněk and Krontorád Koutná, Irena},
   article_location = {Philadelphia},
   article_number = {24},
   doi = {http://dx.doi.org/10.1080/15384101.2015.1106760},
   keywords = {Cell cycle; Chromatin; DNA replication; H3K56ac; Mammalian cells; Nucleosome},
   language = {eng},
   issn = {1538-4101},
   journal = {Cell Cycle},
   title = {Cell cycle-dependent changes in H3K56ac in human cells.},
   url = {http://www.tandfonline.com/doi/abs/10.1080/15384101.2015.1106760},
   volume = {14},
   year = {2015}
}

TY  - JOUR
ID  - 1322928
AU  - Stejskal, Stanislav - Štěpka, Karel - Tesařová, Lenka - Stejskal, Karel - Matějková, Martina - Šimara, Pavel - Zdráhal, Zbyněk - Krontorád Koutná, Irena
PY  - 2015
TI  - Cell cycle-dependent changes in H3K56ac in human cells.
JF  - Cell Cycle
VL  - 14
IS  - 24
SP  - 3851-3863
EP  - 3851-3863
PB  - Taylor&Francis Inc
SN  - 15384101
KW  - Cell cycle
KW  - Chromatin
KW  - DNA replication
KW  - H3K56ac
KW  - Mammalian cells
KW  - Nucleosome
UR  - http://www.tandfonline.com/doi/abs/10.1080/15384101.2015.1106760
L2  - http://www.tandfonline.com/doi/abs/10.1080/15384101.2015.1106760
N2  - The incorporation of histone H3 with an acetylated lysine 56 (H3K56ac) into the nucleosome is important for chromatin remodeling and serves as a marker of new nucleosomes during DNA replication and repair in yeast. However, in human cells, the level of H3K56ac is greatly reduced, and its role during the cell cycle is controversial. Our aim was to determine the potential of H3K56ac to regulate cell cycle progression in different human cell lines. A significant increase in the number of H3K56ac foci, but not in H3K56ac protein levels, was observed during the S and G2 phases in cancer cell lines, but was not observed in embryonic stem cell lines. Despite this increase, the H3K56ac signal was not present in late replication chromatin, and H3K56ac protein levels did not decrease after the inhibition of DNA replication. H3K56ac was not tightly associated with the chromatin and was primarily localized to active chromatin regions. Our results support the role of H3K56ac in transcriptionally active chromatin areas but do not confirm H3K56ac as a marker of newly synthetized nucleosomes in DNA replication.
ER  -

STEJSKAL, Stanislav, Karel ŠTĚPKA, Lenka TESAŘOVÁ, Karel STEJSKAL, Martina MATĚJKOVÁ, Pavel ŠIMARA, Zbyněk ZDRÁHAL and Irena KRONTORÁD KOUTNÁ. Cell cycle-dependent changes in H3K56ac in human cells. \textit{Cell Cycle}. Philadelphia: Taylor\&{}Francis Inc, 2015, vol.~14, No~24, p.~3851-3863. ISSN~1538-4101. Available from: https://dx.doi.org/10.1080/15384101.2015.1106760.

Detailed Information on Publication Record