Novel LinA Type 3 delta-Hexachlorocyclohexane Dehydrochlorinase

J 2015

Novel LinA Type 3 delta-Hexachlorocyclohexane Dehydrochlorinase

SHRIVASTAVA, Nidhi, Zbyněk PROKOP and Ashwani KUMAR

Basic information

Original name

Novel LinA Type 3 delta-Hexachlorocyclohexane Dehydrochlorinase

Authors

SHRIVASTAVA, Nidhi (356 India), Zbyněk PROKOP (203 Czech Republic, belonging to the institution) and Ashwani KUMAR (356 India, guarantor)

Edition

Applied and Environmental Microbiology, WASHINGTON, DC (USA), AMER SOC MICROBIOLOGY, 2015, 0099-2240

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.823

RIV identification code

RIV/00216224:14310/15:00085588

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1128/AEM.01683-15

UT WoS

000363462900021

Keywords in English

HALOALKANE DEHALOGENASE LINB; SPHINGOBIUM-INDICUM B90A; GAMMA-HEXACHLOROCYCLOHEXANE; BETA-HEXACHLOROCYCLOHEXANE; ALPHA-HEXACHLOROCYCLOHEXANE; DEGRADATION; TRANSFORMATION; ISOMERS

Abstract

V originále

LinA is the first enzyme of the microbial degradation pathway of a chlorinated insecticide, hexachlorocyclohexane (HCH), and mediates the dehydrochlorination of alpha-, gamma-, and delta-HCH. Its two variants, LinA type 1 and LinA type 2, which differ at 10 out of 156 amino acid residues, have been described. Their activities for the metabolism of different HCH isomers differ considerably but overall are high for gamma-HCH, moderate for alpha-HCH, low for delta-HCH, and lacking for beta-HCH. Here, we describe the characterization of a new variant of this enzyme, LinA type 3, whose gene was identified from the metagenome of an HCH-contaminated soil sample. Its deduced primary structure in the region spanning amino acid residues 1 to 147 of the protein exhibits 17 and 12 differences from LinA type 1 and LinA type 2, respectively. In addition, the residues GIHFAPS, present at the region spanning residues 148 to 154 in both LinA type 1 and LinA type 2, are deleted in LinA type 3. The activity of LinA type 3 for the metabolism of delta-HCH is several orders of magnitude higher than that of LinA type 1 or LinA type 2 and can be useful for improvement of the metabolism of delta-HCH.

Links

LM2011028, research and development project

Name: RECETOX ? Národní infrastruktura pro výzkum toxických látek v prostředí

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

SHRIVASTAVA, Nidhi, Zbyněk PROKOP and Ashwani KUMAR. Novel LinA Type 3 delta-Hexachlorocyclohexane Dehydrochlorinase. Applied and Environmental Microbiology. WASHINGTON, DC (USA): AMER SOC MICROBIOLOGY, 2015, vol. 81, No 21, p. 7553-7559. ISSN 0099-2240. Available from: https://dx.doi.org/10.1128/AEM.01683-15.

@article{1322953,
   author = {Shrivastava, Nidhi and Prokop, Zbyněk and Kumar, Ashwani},
   article_location = {WASHINGTON, DC (USA)},
   article_number = {21},
   doi = {http://dx.doi.org/10.1128/AEM.01683-15},
   keywords = {HALOALKANE DEHALOGENASE LINB; SPHINGOBIUM-INDICUM B90A; GAMMA-HEXACHLOROCYCLOHEXANE; BETA-HEXACHLOROCYCLOHEXANE; ALPHA-HEXACHLOROCYCLOHEXANE; DEGRADATION; TRANSFORMATION; ISOMERS},
   language = {eng},
   issn = {0099-2240},
   journal = {Applied and Environmental Microbiology},
   title = {Novel LinA Type 3 delta-Hexachlorocyclohexane Dehydrochlorinase},
   url = {http://aem.asm.org/content/81/21/7553},
   volume = {81},
   year = {2015}
}

TY  - JOUR
ID  - 1322953
AU  - Shrivastava, Nidhi - Prokop, Zbyněk - Kumar, Ashwani
PY  - 2015
TI  - Novel LinA Type 3 delta-Hexachlorocyclohexane Dehydrochlorinase
JF  - Applied and Environmental Microbiology
VL  - 81
IS  - 21
SP  - 7553-7559
EP  - 7553-7559
PB  - AMER SOC MICROBIOLOGY
SN  - 00992240
KW  - HALOALKANE DEHALOGENASE LINB
KW  - SPHINGOBIUM-INDICUM B90A
KW  - GAMMA-HEXACHLOROCYCLOHEXANE
KW  - BETA-HEXACHLOROCYCLOHEXANE
KW  - ALPHA-HEXACHLOROCYCLOHEXANE
KW  - DEGRADATION
KW  - TRANSFORMATION
KW  - ISOMERS
UR  - http://aem.asm.org/content/81/21/7553
L2  - http://aem.asm.org/content/81/21/7553
N2  - LinA is the first enzyme of the microbial degradation pathway of a chlorinated insecticide, hexachlorocyclohexane (HCH), and mediates the dehydrochlorination of alpha-, gamma-, and delta-HCH. Its two variants, LinA type 1 and LinA type 2, which differ at 10 out of 156 amino acid residues, have been described. Their activities for the metabolism of different HCH isomers differ considerably but overall are high for gamma-HCH, moderate for alpha-HCH, low for delta-HCH, and lacking for beta-HCH. Here, we describe the characterization of a new variant of this enzyme, LinA type 3, whose gene was identified from the metagenome of an HCH-contaminated soil sample. Its deduced primary structure in the region spanning amino acid residues 1 to 147 of the protein exhibits 17 and 12 differences from LinA type 1 and LinA type 2, respectively. In addition, the residues GIHFAPS, present at the region spanning residues 148 to 154 in both LinA type 1 and LinA type 2, are deleted in LinA type 3. The activity of LinA type 3 for the metabolism of delta-HCH is several orders of magnitude higher than that of LinA type 1 or LinA type 2 and can be useful for improvement of the metabolism of delta-HCH.
ER  -

SHRIVASTAVA, Nidhi, Zbyněk PROKOP and Ashwani KUMAR. Novel LinA Type 3 delta-Hexachlorocyclohexane Dehydrochlorinase. \textit{Applied and Environmental Microbiology}. WASHINGTON, DC (USA): AMER SOC MICROBIOLOGY, 2015, vol.~81, No~21, p.~7553-7559. ISSN~0099-2240. Available from: https://dx.doi.org/10.1128/AEM.01683-15.

Detailed Information on Publication Record