Detection of Airborne Inoculum of Hymenoscyphus fraxineus and
H. albidus during Seasonal Fluctuations Associated with Absence
of Apothecia

J 2016

Detection of Airborne Inoculum of Hymenoscyphus fraxineus and H. albidus during Seasonal Fluctuations Associated with Absence of Apothecia

DVOŘÁK, Miloň, Gabriela ROTKOVÁ and Leticia BOTELLA

Basic information

Original name

Detection of Airborne Inoculum of Hymenoscyphus fraxineus and H. albidus during Seasonal Fluctuations Associated with Absence of Apothecia

Authors

DVOŘÁK, Miloň (203 Czech Republic), Gabriela ROTKOVÁ (203 Czech Republic, belonging to the institution) and Leticia BOTELLA (724 Spain)

Edition

Forests, 2016, 1999-4907

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40102 Forestry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.951

RIV identification code

RIV/00216224:14310/16:00094194

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/f7010001

UT WoS

000369493400004

Keywords in English

Chalara fraxinea; ash dieback; qPCR detection; spore trap; Fraxinus; leaf wetness

Abstract

V originále

Hymenoscyphus fraxineus is an invasive fungal species causing the most serious disease of ashes (Fraxinus spp.) in Europe—ash dieback. The biology of this fungus is not totally elucidated, neither its relation to the saprophytic species Hymenoscyphus albidus, native in Europe. Our study is focused on the description of seasonal spore dispersal of both fungi and its relation to meteorological conditions, which is needed for more precise and effective control of the disease. For this experiment one long time infected mixed forest in the SE Czech Republic was chosen. A seven-day automatic volumetric spore trap and a weather station were installed to continuously sample the aerospora from April to October 2014. In seven periods a rotating arm spore trap was also used to obtain 48-h air samples to compare the efficiency of these two types of air samplers. Air samples were evaluated solely by qPCR with a very low detection limit. Results show co-occurrence of inoculum of both fungi throughout the entire sampling period with peak levels in August. The origin of the inoculum sampled in the periods without apothecia is discussed. Air-inoculum occurrence of both fungi is significantly correlated with each other, suggesting their coexistence in this forest.

Citovat

DVOŘÁK, Miloň, Gabriela ROTKOVÁ and Leticia BOTELLA. Detection of Airborne Inoculum of Hymenoscyphus fraxineus and H. albidus during Seasonal Fluctuations Associated with Absence of Apothecia. Forests. 2016, vol. 7, No 1, p. nestránkováno, 13 pp. ISSN 1999-4907. Available from: https://dx.doi.org/10.3390/f7010001.

@article{1323234,
   author = {Dvořák, Miloň and Rotková, Gabriela and Botella, Leticia},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/f7010001},
   keywords = {Chalara fraxinea; ash dieback; qPCR detection; spore trap; Fraxinus; leaf wetness},
   language = {eng},
   issn = {1999-4907},
   journal = {Forests},
   title = {Detection of Airborne Inoculum of Hymenoscyphus fraxineus and H. albidus during Seasonal Fluctuations Associated with Absence of Apothecia},
   url = {http://www.mdpi.com/1999-4907/7/1/1},
   volume = {7},
   year = {2016}
}

TY  - JOUR
ID  - 1323234
AU  - Dvořák, Miloň - Rotková, Gabriela - Botella, Leticia
PY  - 2016
TI  - Detection of Airborne Inoculum of Hymenoscyphus fraxineus and H. albidus during Seasonal Fluctuations Associated with Absence of Apothecia
JF  - Forests
VL  - 7
IS  - 1
SP  - nestránkováno
EP  - nestránkováno
SN  - 19994907
KW  - Chalara fraxinea
KW  - ash dieback
KW  - qPCR detection
KW  - spore trap
KW  - Fraxinus
KW  - leaf wetness
UR  - http://www.mdpi.com/1999-4907/7/1/1
L2  - http://www.mdpi.com/1999-4907/7/1/1
N2  - Hymenoscyphus fraxineus is an invasive fungal species causing the most serious disease of ashes (Fraxinus spp.) in Europe—ash dieback. The biology of this fungus is not totally elucidated, neither its relation to the saprophytic species Hymenoscyphus albidus, native in Europe. Our study is focused on the description of seasonal spore dispersal of both fungi and its relation to meteorological conditions, which is needed for more precise and effective control of the disease. For this experiment one long time infected mixed forest in the SE Czech Republic was chosen. A seven-day automatic volumetric spore trap and a weather station were installed to continuously sample the aerospora from April to October 2014. In seven periods a rotating arm spore trap was also used to obtain 48-h air samples to compare the efficiency of these two types of air samplers. Air samples were evaluated solely by qPCR with a very low detection limit. Results show co-occurrence of inoculum of both fungi throughout the entire sampling period with peak levels in August. The origin of the inoculum sampled in the periods without apothecia is discussed. Air-inoculum occurrence of both fungi is significantly correlated with each other, suggesting their coexistence in this forest.
ER  -

DVOŘÁK, Miloň, Gabriela ROTKOVÁ and Leticia BOTELLA. Detection of Airborne Inoculum of Hymenoscyphus fraxineus and H. albidus during Seasonal Fluctuations Associated with Absence of Apothecia. \textit{Forests}. 2016, vol.~7, No~1, p.~nestránkováno, 13 pp. ISSN~1999-4907. Available from: https://dx.doi.org/10.3390/f7010001.

Detailed Information on Publication Record