BONCZEK, Ondřej, Alena ŽÁKOVSKÁ, Lýdia HLINECKÁ and Omar ŠERÝ. Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic. Annals of Agricultural and Environmental Medicine. Institute of Agricultural Medicine, 2015, vol. 22, No 4, p. 637-641. ISSN 1232-1966. Available from: https://dx.doi.org/10.5604/12321966.1185766.
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Basic information
Original name Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic
Authors BONCZEK, Ondřej (203 Czech Republic, belonging to the institution), Alena ŽÁKOVSKÁ (203 Czech Republic, belonging to the institution), Lýdia HLINECKÁ (203 Czech Republic, belonging to the institution) and Omar ŠERÝ (203 Czech Republic, guarantor, belonging to the institution).
Edition Annals of Agricultural and Environmental Medicine, Institute of Agricultural Medicine, 2015, 1232-1966.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Poland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 0.895
RIV identification code RIV/00216224:14310/15:00085836
Organization unit Faculty of Science
Doi http://dx.doi.org/10.5604/12321966.1185766
UT WoS 000369231000011
Keywords in English Borrelia burgdorferi s. l.; Borrelia spielmanii; Ixodes ricinus; genotyping; RealTime PCR; DNA sequencing
Tags AKR, rivok
Changed by Changed by: prof. RNDr. Omar Šerý, Ph.D., učo 18120. Changed: 2/3/2016 19:29.
Abstract
During 2008-2012, a total of 466 ticks Ixodes ricinus removed from humans were collected and tested for the presence of Borrelia burgdorferi sensu lato (Bbsl). Ticks were collected in all districts of the South Moravian region of the Czech Republic (CZ). The aim of this study was to determine the infestation of Bbsl in ticks Ixodes ricinus and the identification of genospecies of Bbsl group by DNA sequencing. DNA isolation from homogenates was performed by UltraClean BloodSpin DNA kit (MoBio) and by automated instrument Prepito (Perkin-Elmer). Detection of spirochetes was carried out by RealTime PCR kit EliGene Borrelia LC (Elisabeth Pharmacon). Finally, all the positive samples were sequenced on an ABI 3130 Genetic Analyzer (Life Technologies) and identified in the BLAST (NCBI) database. A total positivity of the samples was 26%. For the first time in the Czech Republic, 5 of the isolated strains were genotyped as Borrelia spielmanii (7.1%). Other representatives of Bbsl were also observed: B. afzelii (70.0%), B. garinii (10.0%), B. valaisiana (8.6%), and B. burgdorferi s. s. (4.3%). A general view of the spreading of Bbsl in the South Moravian region was demonstrated. The most interesting result of the study is the finding of B. spielmanii for the first time in this region.
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