| JIRSOVÁ, Dagmar, Jiří VOREL, Pavel ROUDNICKÝ, Hynek STRNAD, Lucie JEDLIČKOVÁ, Hana DVOŘÁKOVÁ, Karolína SKIPALOVÁ, Libor MIKEŠ, Petr BROŽ, Roman LEONTOVYČ, Ewa DZIKA, Božena KOUBKOVÁ, Milan GELNAR, Martin KAŠNÝ and Jana ILGOVÁ. Monogenea: From sequences to molecules…. In 4th Workshop of European Centre of IchthyoParasitology. 2015. ISBN 978-80-210-8016-4. |
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@proceedings{1324000,
author = {Jirsová, Dagmar and Vorel, Jiří and Roudnický, Pavel and Strnad, Hynek and Jedličková, Lucie and Dvořáková, Hana and Skipalová, Karolína and Mikeš, Libor and Brož, Petr and Leontovyč, Roman and Dzika, Ewa and Koubková, Božena and Gelnar, Milan and Kašný, Martin and Ilgová, Jana},
booktitle = {4th Workshop of European Centre of IchthyoParasitology},
keywords = {Eudiplozoon nipponicum; Monogenea; next generation sequencing; transcriptome; genome},
language = {eng},
isbn = {978-80-210-8016-4},
title = {Monogenea: From sequences to molecules…},
year = {2015}
}
TY - CONF
ID - 1324000
AU - Jirsová, Dagmar - Vorel, Jiří - Roudnický, Pavel - Strnad, Hynek - Jedličková, Lucie - Dvořáková, Hana - Skipalová, Karolína - Mikeš, Libor - Brož, Petr - Leontovyč, Roman - Dzika, Ewa - Koubková, Božena - Gelnar, Milan - Kašný, Martin - Ilgová, Jana
PY - 2015
TI - Monogenea: From sequences to molecules…
SN - 9788021080164
KW - Eudiplozoon nipponicum
KW - Monogenea
KW - next generation sequencing
KW - transcriptome
KW - genome
N2 - In a first part of our pilot study of monogeneans molecules we adopted the NGS techniques in order to get the high quality “monogenean-polyopisthocotylean genome/transcriptome matrix”. In a second part of our study this platform was used for the identification of the monogeneans´ dominant protein molecules followed by their further molecular/biochemical characterization. For this purpose we adopted Eudiplozoon nipponicum as a experimental model species; the representative of blood-feeding monogeneans (family Diplozoidae) of fresh water fishes, the ectoparasite from the gills of Cyprinus carpio. The bioinformatic analyses of genomic sequence data were performed; the first genome assembly of 98 mil of E. nipponicum DNA (3 libraries) sequence reads was done and the calculation of genome size was initiated. Up to now, the low coverage (6%) was reached and therefore the distorted number of bases of whole E. nipponicum genome (400 Gb) was estimated.
ER -
JIRSOVÁ, Dagmar, Jiří VOREL, Pavel ROUDNICKÝ, Hynek STRNAD, Lucie JEDLIČKOVÁ, Hana DVOŘÁKOVÁ, Karolína SKIPALOVÁ, Libor MIKEŠ, Petr BROŽ, Roman LEONTOVYČ, Ewa DZIKA, Božena KOUBKOVÁ, Milan GELNAR, Martin KAŠNÝ and Jana ILGOVÁ. Monogenea: From sequences to molecules…. In \textit{4th Workshop of European Centre of IchthyoParasitology}. 2015. ISBN~978-80-210-8016-4.
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