Detailed Information on Publication Record
2013
Analysis of plasmid dna isolated from strains of Staphylococcus aureus using artificial nucleases
ZOVČÁKOVÁ, Monika, Alena ŠPANOVÁ, Bohuslav RITTICH, Roman PANTŮČEK, Jiří DOŠKAŘ et. al.Basic information
Original name
Analysis of plasmid dna isolated from strains of Staphylococcus aureus using artificial nucleases
Authors
ZOVČÁKOVÁ, Monika, Alena ŠPANOVÁ, Bohuslav RITTICH, Roman PANTŮČEK and Jiří DOŠKAŘ
Edition
Barceló congresos, Barcelona, Spain, 2013
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Spain
Confidentiality degree
není předmětem státního či obchodního tajemství
Organization unit
Faculty of Science
Keywords (in Czech)
Artificiálne nukleázy; plasmid; Staphylococcus aureus
Změněno: 16/1/2016 20:53, prof. RNDr. Roman Pantůček, Ph.D.
Abstract
V originále
Hydrolysis of nucleic acids mediated by artificial nucleases is very important goal in molecular biology, biotechnology and medicine. Some metal and lanthanide ions and their soluble or insoluble complexes (immobilized on solid particles) are attractive as catalysts for DNA cleavage. The aim of this work was to use of neodymium ions for cleavage of plasmid DNAs. Plasmid DNAs originated from strains of Staphylococcus aureus isolated from clinical and food samples were treated by neodymium ions (Nd3+). Plasmid pUC19 was used as model molecule and linearized pUC19/EcoRI was used as the control of the presence of linear form. Cleavage of plasmid DNA was carried out at different temperatures in HEPES (0.05M) buffer. The reaction time was up to 4 hours. The reaction was stopped with 0.5 M EDTA (pH 8.0). Plasmid DNA cleavage efficiency was evaluated by convert the supercoiled circular (ccc) form of plasmid DNA into nicked circular form (oc) and linear (lin) one and was monitored on the gel according to the shift of their mobilities.