MiR-205 functions as a tumor suppressor in adenocarcinoma and
an oncogene in squamous cell carcinoma of esophagus

J 2016

MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus

HÉŽOVÁ, Renata, Alena KOVAŘÍKOVÁ, Josef SROVNAL, Milada ZEMANOVÁ, Tomáš HARUSTIAK et. al.

Basic information

Original name

MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus

Authors

HÉŽOVÁ, Renata (203 Czech Republic, belonging to the institution), Alena KOVAŘÍKOVÁ (203 Czech Republic, belonging to the institution), Josef SROVNAL (203 Czech Republic), Milada ZEMANOVÁ (203 Czech Republic), Tomáš HARUSTIAK (703 Slovakia), Jiří EHRMANN (203 Czech Republic), Marian HAJDÚCH (203 Czech Republic), Milana ŠACHLOVÁ (203 Czech Republic), Marek SVOBODA (203 Czech Republic) and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Tumor Biology, Dordrecht, Springer, 2016, 1010-4283

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.650

RIV identification code

RIV/00216224:14740/16:00088840

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1007/s13277-015-4656-8

UT WoS

000376464700100

Keywords in English

Esophageal adenocarcinoma; Esophageal squamous cell carcinoma; miR-205; Tumor suppressor; Oncogene

Abstract

V originále

Esophageal cancer is a malignant disease with poor prognosis, increasing incidence, and ineffective treatment options. MicroRNAs are post-transcriptional regulators of gene expression involved in many biological processes including carcinogenesis. We determined miR-205 expression levels in tumor/non-tumor tissues of 45 esophageal cancer patients using qPCR and found that decreased level of miR-205 in tumor tissue correlates with poor overall survival in esophageal adenocarcinoma patients. Further, we observed significantly higher levels of miR-205 in tumor tissue of esophageal squamous cell carcinoma. Ectopic overexpression of miR-205 in adenocarcinoma cell line SK-GT-4 led to decreased cell proliferation, cell cycle arrest in G1, and decreased migration ability. Conversely, in squamous cell line KYSE-150, same effects like inhibition of proliferation, migration, and colony-forming potential and cell cycle arrest in G2 were observed after silencing of miR-205. We performed global gene expression profiling and revealed that suppressive functioning of miR-205 in adenocarcinoma could be realized through regulation of epithelial-mesenchymal transition (EMT), whereas oncogenic in squamous cell carcinoma by regulation of metalloproteinase 10. Our results suggest that miR-205 could serve as biomarker in esophageal cancer and acts as a tumor suppressor in esophageal adenocarcinoma and oncogene in esophageal squamous cell carcinoma.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

NT13547, research and development project

Name: Studium mikroRNA a genů asociovaných s procesem epiteliálně-mezenchymální tranzice jako potenciálních markerů pro predikci rizika a časný záchyt metastazování u pacientů s renálním karcinomem

NV15-34678A, research and development project

Name: Molekulární prognostické a prediktivní faktory u pacientů s metastatickým renálním karcinomem léčených tyrozinkinázovými inhibitory

Citovat

HÉŽOVÁ, Renata, Alena KOVAŘÍKOVÁ, Josef SROVNAL, Milada ZEMANOVÁ, Tomáš HARUSTIAK, Jiří EHRMANN, Marian HAJDÚCH, Milana ŠACHLOVÁ, Marek SVOBODA and Ondřej SLABÝ. MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus. Tumor Biology. Dordrecht: Springer, 2016, vol. 37, No 6, p. 8007-8018. ISSN 1010-4283. Available from: https://dx.doi.org/10.1007/s13277-015-4656-8.

@article{1331432,
   author = {Héžová, Renata and Kovaříková, Alena and Srovnal, Josef and Zemanová, Milada and Harustiak, Tomáš and Ehrmann, Jiří and Hajdúch, Marian and Šachlová, Milana and Svoboda, Marek and Slabý, Ondřej},
   article_location = {Dordrecht},
   article_number = {6},
   doi = {http://dx.doi.org/10.1007/s13277-015-4656-8},
   keywords = {Esophageal adenocarcinoma; Esophageal squamous cell carcinoma; miR-205; Tumor suppressor; Oncogene},
   language = {eng},
   issn = {1010-4283},
   journal = {Tumor Biology},
   title = {MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus},
   url = {http://link.springer.com/article/10.1007%2Fs13277-015-4656-8},
   volume = {37},
   year = {2016}
}

TY  - JOUR
ID  - 1331432
AU  - Héžová, Renata - Kovaříková, Alena - Srovnal, Josef - Zemanová, Milada - Harustiak, Tomáš - Ehrmann, Jiří - Hajdúch, Marian - Šachlová, Milana - Svoboda, Marek - Slabý, Ondřej
PY  - 2016
TI  - MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus
JF  - Tumor Biology
VL  - 37
IS  - 6
SP  - 8007-8018
EP  - 8007-8018
PB  - Springer
SN  - 10104283
KW  - Esophageal adenocarcinoma
KW  - Esophageal squamous cell carcinoma
KW  - miR-205
KW  - Tumor suppressor
KW  - Oncogene
UR  - http://link.springer.com/article/10.1007%2Fs13277-015-4656-8
L2  - http://link.springer.com/article/10.1007%2Fs13277-015-4656-8
N2  - Esophageal cancer is a malignant disease with poor prognosis, increasing incidence, and ineffective treatment options. MicroRNAs are post-transcriptional regulators of gene expression involved in many biological processes including carcinogenesis. We determined miR-205 expression levels in tumor/non-tumor tissues of 45 esophageal cancer patients using qPCR and found that decreased level of miR-205 in tumor tissue correlates with poor overall survival in esophageal adenocarcinoma patients. Further, we observed significantly higher levels of miR-205 in tumor tissue of esophageal squamous cell carcinoma. Ectopic overexpression of miR-205 in adenocarcinoma cell line SK-GT-4 led to decreased cell proliferation, cell cycle arrest in G1, and decreased migration ability. Conversely, in squamous cell line KYSE-150, same effects like inhibition of proliferation, migration, and colony-forming potential and cell cycle arrest in G2 were observed after silencing of miR-205. We performed global gene expression profiling and revealed that suppressive functioning of miR-205 in adenocarcinoma could be realized through regulation of epithelial-mesenchymal transition (EMT), whereas oncogenic in squamous cell carcinoma by regulation of metalloproteinase 10. Our results suggest that miR-205 could serve as biomarker in esophageal cancer and acts as a tumor suppressor in esophageal adenocarcinoma and oncogene in esophageal squamous cell carcinoma.
ER  -

HÉŽOVÁ, Renata, Alena KOVAŘÍKOVÁ, Josef SROVNAL, Milada ZEMANOVÁ, Tomáš HARUSTIAK, Jiří EHRMANN, Marian HAJDÚCH, Milana ŠACHLOVÁ, Marek SVOBODA and Ondřej SLABÝ. MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus. \textit{Tumor Biology}. Dordrecht: Springer, 2016, vol.~37, No~6, p.~8007-8018. ISSN~1010-4283. Available from: https://dx.doi.org/10.1007/s13277-015-4656-8.

Detailed Information on Publication Record