Interaction of RECQ4 and MCM10 is important for efficient DNA
replication origin firing in human cells

J 2015

Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells

KLISZCZAK, Maciej, Hana SEDLÁČKOVÁ, Ganesha P. PITCHAI, Werner W. STREICHER, Lumír KREJČÍ et. al.

Basic information

Original name

Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells

Authors

KLISZCZAK, Maciej (208 Denmark), Hana SEDLÁČKOVÁ (203 Czech Republic, belonging to the institution), Ganesha P. PITCHAI (208 Denmark), Werner W. STREICHER (208 Denmark), Lumír KREJČÍ (203 Czech Republic, guarantor, belonging to the institution) and Ian D. HICKSON (208 Denmark)

Edition

Oncotarget, New York, Impact Journals LLC, 2015, 1949-2553

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.008

RIV identification code

RIV/00216224:14110/15:00081496

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.18632/oncotarget.6342

UT WoS

000366115500008

Keywords in English

DNA replication; RecQ helicases; minichromosome maintenance proteins; Chromosome Section

Abstract

V originále

DNA replication is a highly coordinated process that is initiated at multiple replication origins in eukaryotes. These origins are bound by the origin recognition complex (ORC), which subsequently recruits the Mcm2-7 replicative helicase in a Cdt1/Cdc6-dependent manner. In budding yeast, two essential replication factors, Sld2 and Mcm10, are then important for the activation of replication origins. In humans, the putative Sld2 homolog, RECQ4, interacts with MCM10. Here, we have identified two mutants of human RECQ4 that are deficient in binding to MCM10. We show that these RECQ4 variants are able to complement the lethality of an avian cell RECQ4 deletion mutant, indicating that the essential function of RECQ4 in vertebrates is unlikely to require binding to MCM10. Nevertheless, we show that the RECQ4-MCM10 interaction is important for efficient replication origin firing.

Links

GAP207/12/2323, research and development project

Name: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Czech Science Foundation

GA13-26629S, research and development project

Name: SUMO a stability genomu

Investor: Czech Science Foundation

MUNI/M/1894/2014, interní kód MU

Name: Development of new MUS81 nuclease inhibitors as chemical biology probe with clinical progression

Investor: Masaryk University, INTERDISCIPLINARY - Interdisciplinary research projects

Citovat

KLISZCZAK, Maciej, Hana SEDLÁČKOVÁ, Ganesha P. PITCHAI, Werner W. STREICHER, Lumír KREJČÍ and Ian D. HICKSON. Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells. Oncotarget. New York: Impact Journals LLC, 2015, vol. 6, No 38, p. 40464-40479. ISSN 1949-2553. Available from: https://dx.doi.org/10.18632/oncotarget.6342.

@article{1331446,
   author = {Kliszczak, Maciej and Sedláčková, Hana and Pitchai, Ganesha P. and Streicher, Werner W. and Krejčí, Lumír and Hickson, Ian D.},
   article_location = {New York},
   article_number = {38},
   doi = {http://dx.doi.org/10.18632/oncotarget.6342},
   keywords = {DNA replication; RecQ helicases; minichromosome maintenance proteins; Chromosome Section},
   language = {eng},
   issn = {1949-2553},
   journal = {Oncotarget},
   title = {Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells},
   volume = {6},
   year = {2015}
}

TY  - JOUR
ID  - 1331446
AU  - Kliszczak, Maciej - Sedláčková, Hana - Pitchai, Ganesha P. - Streicher, Werner W. - Krejčí, Lumír - Hickson, Ian D.
PY  - 2015
TI  - Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells
JF  - Oncotarget
VL  - 6
IS  - 38
SP  - 40464-40479
EP  - 40464-40479
PB  - Impact Journals LLC
SN  - 19492553
KW  - DNA replication
KW  - RecQ helicases
KW  - minichromosome maintenance proteins
KW  - Chromosome Section
N2  - DNA replication is a highly coordinated process that is initiated at multiple replication origins in eukaryotes. These origins are bound by the origin recognition complex (ORC), which subsequently recruits the Mcm2-7 replicative helicase in a Cdt1/Cdc6-dependent manner. In budding yeast, two essential replication factors, Sld2 and Mcm10, are then important for the activation of replication origins. In humans, the putative Sld2 homolog, RECQ4, interacts with MCM10. Here, we have identified two mutants of human RECQ4 that are deficient in binding to MCM10. We show that these RECQ4 variants are able to complement the lethality of an avian cell RECQ4 deletion mutant, indicating that the essential function of RECQ4 in vertebrates is unlikely to require binding to MCM10. Nevertheless, we show that the RECQ4-MCM10 interaction is important for efficient replication origin firing.
ER  -

KLISZCZAK, Maciej, Hana SEDLÁČKOVÁ, Ganesha P. PITCHAI, Werner W. STREICHER, Lumír KREJČÍ and Ian D. HICKSON. Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells. \textit{Oncotarget}. New York: Impact Journals LLC, 2015, vol.~6, No~38, p.~40464-40479. ISSN~1949-2553. Available from: https://dx.doi.org/10.18632/oncotarget.6342.

Detailed Information on Publication Record