Regulatory genes in HN SCC cell cultures

HORAKOVA, Z., Hana BINKOVÁ, Rom KOSTŘICA, Jaromír GUMULEC and Michal MASAŘÍK. Regulatory genes in HN SCC cell cultures. In 3rd Congress of European ORL-HNS. 2015.

Basic information

• Original name: Regulatory genes in HN SCC cell cultures
• Name in Czech: Regulační geny v buněčných liniích spinocelulárních karcinomů hlavy a krku
• Authors: HORAKOVA, Z. (203 Czech Republic), Hana BINKOVÁ (203 Czech Republic, guarantor, belonging to the institution), Rom KOSTŘICA (203 Czech Republic, belonging to the institution), Jaromír GUMULEC (203 Czech Republic, belonging to the institution) and Michal MASAŘÍK (203 Czech Republic, belonging to the institution).
• Edition: 3rd Congress of European ORL-HNS, 2015.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/15:00087474
• Organization unit: Faculty of Medicine
• Keywords (in Czech): nádory; hlava; krk; buněčné linie
• Keywords in English: cancer; head; neck; cell lines
• Tags: EL OK

Abstract

The incidence of malignities of head and neck in the Czech Republic is found about three thousand new cases per year. Their agressive biological behaviour with high tendency to relapse is presented by an extensive morbidity and mortality mostly on account of a late diagnostic of advanced tumours. Our long term project addresses the analysis of wide scale of regulatory proteins (EGFR, EGF, Ki-67, Fos, Jun, p53, MT, MMP, NF-kappaB, IGFII and BGMIV) with aim to find a suitable molecular predictors of head and neck cancer which might become a helpful tool in treatment management and thus contribute to improvement of therapeutic results. In the running prospective study the cell cultures from bioptic tumour tissue samples of spinocellular head and neck carcinomas /SCCHN/ are being cultivated for assessment of apoptosis- and cell cycle-related genes expression. In this paper the expression of the following genes was analysed: Bax, Bcl-2, p53 by qRT-PCR using Taqman probes and relative ddCt method, and standardized to beta-actin as a housekeeping gene. Non-tumour tissue was used as a relative control. Cell growth was analysed using impedance-based real-time cell growth monitorring system. Our preliminary results in over 100 patients determined significant differences in the expression of some of the assesed regulatory genes. These data suggest inhibition of apoptosis as a way to promote tumorigenesis, cell growth regulation is distinctly affected in this primary cell lines, confirmed by significantly differential growth of tumour and non-tumour primary cell cultures.

Links

• NT14337, research and development project: Name: Studium a charakterizace primárních nádorových buněčných linií spinocelulárních karcinomů v oblasti hlavy a krku a jejich maligní potenciál.

Investor: Ministry of Health of the CR