Different Doxorubicin Formulations Affect Plasma
4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde
Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat

J 2015

Different Doxorubicin Formulations Affect Plasma 4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat

HLAVÁČOVÁ, Miroslava, Jaromír GUMULEC, Tibor STRAČINA, Michaela FOJTŮ, Martina RAUDENSKÁ et. al.

Basic information

Original name

Different Doxorubicin Formulations Affect Plasma 4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat

Authors

HLAVÁČOVÁ, Miroslava (703 Slovakia, belonging to the institution), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Tibor STRAČINA (703 Slovakia, belonging to the institution), Michaela FOJTŮ (203 Czech Republic, belonging to the institution), Martina RAUDENSKÁ (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Marie NOVÁKOVÁ (203 Czech Republic, belonging to the institution) and Hana PAULOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Physiological Research, Praha, Fyziologický ústav AV ČR, 2015, 0862-8408

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.643

RIV identification code

RIV/00216224:14110/15:00086241

Organization unit

Faculty of Medicine

UT WoS

000372421900012

Keywords in English

Doxorubicin; Drug nanotransporters; Oxidative stress; 4-hydroxy-2-nonenal; Thioredoxin reductase 2; Aldehyde dehydrogenase 3A1

Abstract

V originále

Increased oxidative stress is indisputably an important mechanism of doxorubicin side effects, especially its cardiotoxicity. To prevent Impairment of non-tumnrous tissue and to Improve the specificity in targeting the tumor tissue, new drug nanotransporters are developed. In many cases predinical therapeutic advantage has been shown when compared with the administration of conventional drug solution. Three forms of doxorubicin - conventional (DOX), encapsulated In liposomes (lipoDOX) and in apoferritin (apoDQX) were applied to Wistar rats. After 24 h exposition, the plasma level of 4-hydroxy-2-nonenal (4-HNE) as a marker of lipoperoxidalion and tissue gene expression of thioredoxln reductase 2 (TXNRDZ} and aldehyde dehydrogenase 3A1 (ALDH3AI) as an important part of antioxidative system were determined. Only conventional DOX significantly increases the level of 4-HNE; encapsulated forms on the other hand show significant decrease in plasma levels of 4-HNE In comparison with DOX. They also cause significant decrease In gene expression of ALDH3A1 and TXNRD2 In liver as a main detoxification organ, and a mild Influence on the expression of these enzymes in left heart ventride as a potential target of toxiclty. Thus, 4-HNE seems to be a good potential biomarker of oxidative stress induced by various forms of doxorubicin.

Links

MUNI/A/1195/2014, interní kód MU

Name: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: chebimo)

Investor: Masaryk University, Category A

MUNI/A/1326/2014, interní kód MU

Name: Kardiovaskulární systém od buňky k lůžku pacienta (Acronym: KASBUNPAC)

Investor: Masaryk University, Category A

Citovat

HLAVÁČOVÁ, Miroslava, Jaromír GUMULEC, Tibor STRAČINA, Michaela FOJTŮ, Martina RAUDENSKÁ, Michal MASAŘÍK, Marie NOVÁKOVÁ and Hana PAULOVÁ. Different Doxorubicin Formulations Affect Plasma 4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat. Physiological Research. Praha: Fyziologický ústav AV ČR, 2015, vol. 64, Suppl. 5, p. "S653"-"S660", 8 pp. ISSN 0862-8408.

@article{1332505,
   author = {Hlaváčová, Miroslava and Gumulec, Jaromír and Stračina, Tibor and Fojtů, Michaela and Raudenská, Martina and Masařík, Michal and Nováková, Marie and Paulová, Hana},
   article_location = {Praha},
   article_number = {Suppl. 5},
   keywords = {Doxorubicin; Drug nanotransporters; Oxidative stress; 4-hydroxy-2-nonenal; Thioredoxin reductase 2; Aldehyde dehydrogenase 3A1},
   language = {eng},
   issn = {0862-8408},
   journal = {Physiological Research},
   title = {Different Doxorubicin Formulations Affect Plasma 4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat},
   volume = {64},
   year = {2015}
}

TY  - JOUR
ID  - 1332505
AU  - Hlaváčová, Miroslava - Gumulec, Jaromír - Stračina, Tibor - Fojtů, Michaela - Raudenská, Martina - Masařík, Michal - Nováková, Marie - Paulová, Hana
PY  - 2015
TI  - Different Doxorubicin Formulations Affect Plasma 4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat
JF  - Physiological Research
VL  - 64
IS  - Suppl. 5
SP  - "S653"-"S660"
EP  - "S653"-"S660"
PB  - Fyziologický ústav AV ČR
SN  - 08628408
KW  - Doxorubicin
KW  - Drug nanotransporters
KW  - Oxidative stress
KW  - 4-hydroxy-2-nonenal
KW  - Thioredoxin reductase 2
KW  - Aldehyde dehydrogenase 3A1
N2  - Increased oxidative stress is indisputably an important mechanism of doxorubicin side effects, especially its cardiotoxicity. To prevent Impairment of non-tumnrous tissue and to Improve the specificity in targeting the tumor tissue, new drug nanotransporters are developed. In many cases predinical therapeutic advantage has been shown when compared with the administration of conventional drug solution. Three forms of doxorubicin - conventional (DOX), encapsulated In liposomes (lipoDOX) and in apoferritin (apoDQX) were applied to Wistar rats. After 24 h exposition, the plasma level of 4-hydroxy-2-nonenal (4-HNE) as a marker of lipoperoxidalion and tissue gene expression of thioredoxln reductase 2 (TXNRDZ} and aldehyde dehydrogenase 3A1 (ALDH3AI) as an important part of antioxidative system were determined. Only conventional DOX significantly increases the level of 4-HNE; encapsulated forms on the other hand show significant decrease in plasma levels of 4-HNE In comparison with DOX. They also cause significant decrease In gene expression of ALDH3A1 and TXNRD2 In liver as a main detoxification organ, and a mild Influence on the expression of these enzymes in left heart ventride as a potential target of toxiclty. Thus, 4-HNE seems to be a good potential biomarker of oxidative stress induced by various forms of doxorubicin.
ER  -

HLAVÁČOVÁ, Miroslava, Jaromír GUMULEC, Tibor STRAČINA, Michaela FOJTŮ, Martina RAUDENSKÁ, Michal MASAŘÍK, Marie NOVÁKOVÁ and Hana PAULOVÁ. Different Doxorubicin Formulations Affect Plasma 4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat. \textit{Physiological Research}. Praha: Fyziologický ústav AV ČR, 2015, vol.~64, Suppl. 5, p.~''S653''-''S660'', 8 pp. ISSN~0862-8408.

Detailed Information on Publication Record