This work is focused on the preparation of gelatin nanofibers modified with salts of oxidized cellulose. Gelatin is a biocompatible, biodegradable protein-based material with high absorption capacity, which is obtained by partial hydrolysis of natural protein - collagen. Oxidized cellulose and its salts are biocompatible as well, biodegradable and they have antibacterial and hemostatic effects. Electrospun novel nanofibers, combining properties of both gelatin and oxycellulose exhibited unique bactericide efficiency. Chemical composition of the fibers was studied by ATR-FTIR spectroscopy and EDX elemental analysis. Obtained results confirmed the presence of oxidized cellulose salt in prepared nanofibers. Diameter and morphology of nanofibers were determined by scanning electron microscopy (SEM). Bactericidal effect was examined by the method of chemical bioluminescence on modified bacterial strain of E. coli. This method proved complete mortality of E. coli in most of cases. Newly prepared material may have a potential application in surgery, regenerative medicine of soft tissue (burns) and in tissue engineering.