| JOUKAL, Marek, Ilona KLUSÁKOVÁ and Petr DUBOVÝ. Direct communication of the spinal subarachnoid space with the rat dorsal root ganglia. Annals of Anatomy - Anatomischer Anzeiger. Jena: Elsevier, 2016, vol. 205, 1 May 2016, p. 9-15. ISSN 0940-9602. Available from: https://dx.doi.org/10.1016/j.aanat.2016.01.004. |
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@article{1335227,
author = {Joukal, Marek and Klusáková, Ilona and Dubový, Petr},
article_location = {Jena},
article_number = {1 May 2016},
doi = {http://dx.doi.org/10.1016/j.aanat.2016.01.004},
keywords = {Subarachnoid space; Cerebrospinal fluid; Dorsal root ganglia; Fluoro-emerald; Immunohistochemistry; Macrophages},
language = {eng},
issn = {0940-9602},
journal = {Annals of Anatomy - Anatomischer Anzeiger},
title = {Direct communication of the spinal subarachnoid space with the rat dorsal root ganglia},
volume = {205},
year = {2016}
}
TY - JOUR
ID - 1335227
AU - Joukal, Marek - Klusáková, Ilona - Dubový, Petr
PY - 2016
TI - Direct communication of the spinal subarachnoid space with the rat dorsal root ganglia
JF - Annals of Anatomy - Anatomischer Anzeiger
VL - 205
IS - 1 May 2016
SP - 9-15
EP - 9-15
PB - Elsevier
SN - 09409602
KW - Subarachnoid space
KW - Cerebrospinal fluid
KW - Dorsal root ganglia
KW - Fluoro-emerald
KW - Immunohistochemistry
KW - Macrophages
N2 - The anatomical position of the subarachnoid space (SAS) in relation to dorsal root ganglia (DRG) and penetration of tracer from the SAS into DRG were investigated. We used intrathecal injection of methylene blue to visualize the anatomical position of the SAS in relation to DRG and immunostaining of dipeptidyl peptidase IV (DPP-IV) for detecting arachnoid limiting the SAS. Intrathecal administration of fluorescent-conjugated dextran (fluoro-emerald; FE) was used to demonstrate direct communication between the SAS and DRG. Intrathecal injection of methylene blue and DPP-IV immunostaining revealed that SAS delimited by the arachnoid was extended up to the capsule of DRG in a fold-like recess that may reach approximately half of the DRG length. The arachnoid was found in direct contact to the neuronal body-rich area in the angle between dorsal root and DRG as well as between spinal nerve roots at DRG. Particles of FE were found in the cells of DRG capsule, satellite glial cells, interstitial space, as well as in small and medium-sized neurons after intrathecal injection. Penetration of FE from the SAS into the DRG induced an immune reaction expressed by colocalization of FE and immunofluorescence indicating antigen-presenting cells (MHC-II+), activated (ED1+) and resident (ED2+) macrophages, and activation of satellite glial cells (GFAP+). Penetration of lumbar-injected FE into the cervical DRG was greater than that into the lumbar DRG after intrathecal injection of FE into the cisterna magna. Our results demonstrate direct communication between DRG and cerebrospinal fluid in the SAS that can create another pathway for possible propagation of inflammatory and signaling molecules from DRG primary affected by peripheral nerve injury into DRG of remote spinal segments.
ER -
JOUKAL, Marek, Ilona KLUSÁKOVÁ and Petr DUBOVÝ. Direct communication of the spinal subarachnoid space with the rat dorsal root ganglia. \textit{Annals of Anatomy - Anatomischer Anzeiger}. Jena: Elsevier, 2016, vol.~205, 1 May 2016, p.~9-15. ISSN~0940-9602. Available from: https://dx.doi.org/10.1016/j.aanat.2016.01.004.
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