Polymer-immobilized ready-to-use recombinant yeast assays for
the detection of endocrine disruptive compounds

J 2015

Polymer-immobilized ready-to-use recombinant yeast assays for the detection of endocrine disruptive compounds

BITTNER, Michal, Sergio JARQUE ORTIZ and Klára HILSCHEROVÁ

Basic information

Original name

Polymer-immobilized ready-to-use recombinant yeast assays for the detection of endocrine disruptive compounds

Authors

BITTNER, Michal (203 Czech Republic, belonging to the institution), Sergio JARQUE ORTIZ (724 Spain, belonging to the institution) and Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Chemosphere, OXFORD (ENGLAND), Elsevier Science, 2015, 0045-6535

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30304 Public and environmental health

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.698

RIV identification code

RIV/00216224:14310/15:00086633

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.chemosphere.2015.02.063

UT WoS

000355882900008

Keywords in English

Recombinant yeast assay; Immobilization; Gelatin; Agar; Environmental monitoring; Endocrine disruption

Abstract

V originále

Recombinant yeast assays (RYAs) constitute a suitable tool for the environmental monitoring of compounds with endocrine disrupting activities, notably estrogenicity and androgenicity. Conventional procedures require yeast reconstitution from frozen stock, which usually takes several days and demands additional equipment With the aim of applying such assays to field studies and making them more accessible to less well-equipped laboratories, we have optimized RYA by the immobilization of Saccharomyces cerevisiae cells in three different polymer matrices gelatin, Bacto agar, and Yeast Extract Peptone Dextrose agar to obtain a ready-to-use version for the fast assessment of estrogenic and androgenic potencies of compounds and environmental samples. Among the three matrices, gelatin showed the best results for both testosterone (androgen receptor yeast strain; AR-RYA) and 17 beta-estradiol (estrogen receptor yeast strain; ER-RYA). AR-RYA was characterized by a lowest observed effect concentration (LOEC), EC50 and induction factor (IF) of 1 nM, 2.2 nM and 51, respectively. The values characterizing ER-RYA were 0.4 nM, 1.8 nM, and 63, respectively. Gelatin immobilization retained yeast viability and sensitivity for more than 90 d of storage at 4 degrees C The use of the immobilized yeast reduced the assay duration to only 3 h without necessity of sterile conditions. Because immobilized RYA can be performed either in multiwell microplates or glass tubes, it allows multiple samples to be tested at once, and easy adaptation to existing portable devices for direct in-field applications.

Links

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

BITTNER, Michal, Sergio JARQUE ORTIZ and Klára HILSCHEROVÁ. Polymer-immobilized ready-to-use recombinant yeast assays for the detection of endocrine disruptive compounds. Chemosphere. OXFORD (ENGLAND): Elsevier Science, 2015, vol. 132, AUGUST, p. 56-62. ISSN 0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2015.02.063.

@article{1335943,
   author = {Bittner, Michal and Jarque Ortiz, Sergio and Hilscherová, Klára},
   article_location = {OXFORD (ENGLAND)},
   article_number = {AUGUST},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2015.02.063},
   keywords = {Recombinant yeast assay; Immobilization; Gelatin; Agar; Environmental monitoring; Endocrine disruption},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Polymer-immobilized ready-to-use recombinant yeast assays for the detection of endocrine disruptive compounds},
   url = {http://www.sciencedirect.com/science/article/pii/S0045653515001873},
   volume = {132},
   year = {2015}
}

TY  - JOUR
ID  - 1335943
AU  - Bittner, Michal - Jarque Ortiz, Sergio - Hilscherová, Klára
PY  - 2015
TI  - Polymer-immobilized ready-to-use recombinant yeast assays for the detection of endocrine disruptive compounds
JF  - Chemosphere
VL  - 132
IS  - AUGUST
SP  - 56-62
EP  - 56-62
PB  - Elsevier Science
SN  - 00456535
KW  - Recombinant yeast assay
KW  - Immobilization
KW  - Gelatin
KW  - Agar
KW  - Environmental monitoring
KW  - Endocrine disruption
UR  - http://www.sciencedirect.com/science/article/pii/S0045653515001873
L2  - http://www.sciencedirect.com/science/article/pii/S0045653515001873
N2  - Recombinant yeast assays (RYAs) constitute a suitable tool for the environmental monitoring of compounds with endocrine disrupting activities, notably estrogenicity and androgenicity. Conventional procedures require yeast reconstitution from frozen stock, which usually takes several days and demands additional equipment With the aim of applying such assays to field studies and making them more accessible to less well-equipped laboratories, we have optimized RYA by the immobilization of Saccharomyces cerevisiae cells in three different polymer matrices gelatin, Bacto agar, and Yeast Extract Peptone Dextrose agar to obtain a ready-to-use version for the fast assessment of estrogenic and androgenic potencies of compounds and environmental samples. Among the three matrices, gelatin showed the best results for both testosterone (androgen receptor yeast strain; AR-RYA) and 17 beta-estradiol (estrogen receptor yeast strain; ER-RYA). AR-RYA was characterized by a lowest observed effect concentration (LOEC), EC50 and induction factor (IF) of 1 nM, 2.2 nM and 51, respectively. The values characterizing ER-RYA were 0.4 nM, 1.8 nM, and 63, respectively. Gelatin immobilization retained yeast viability and sensitivity for more than 90 d of storage at 4 degrees C The use of the immobilized yeast reduced the assay duration to only 3 h without necessity of sterile conditions. Because immobilized RYA can be performed either in multiwell microplates or glass tubes, it allows multiple samples to be tested at once, and easy adaptation to existing portable devices for direct in-field applications.
ER  -

BITTNER, Michal, Sergio JARQUE ORTIZ and Klára HILSCHEROVÁ. Polymer-immobilized ready-to-use recombinant yeast assays for the detection of endocrine disruptive compounds. \textit{Chemosphere}. OXFORD (ENGLAND): Elsevier Science, 2015, vol.~132, AUGUST, p.~56-62. ISSN~0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2015.02.063.

Detailed Information on Publication Record