Detailed Information on Publication Record
2015
Retinoid compounds associated with water blooms dominated by Microcystis species
JAVŮREK, Jakub, Eliška SYCHROVÁ, Marie SMUTNÁ, Michal BITTNER, Jiří KOHOUTEK et. al.Basic information
Original name
Retinoid compounds associated with water blooms dominated by Microcystis species
Authors
JAVŮREK, Jakub (203 Czech Republic, belonging to the institution), Eliška SYCHROVÁ (203 Czech Republic, belonging to the institution), Marie SMUTNÁ (203 Czech Republic, belonging to the institution), Michal BITTNER (203 Czech Republic, belonging to the institution), Jiří KOHOUTEK (203 Czech Republic, belonging to the institution), Ondřej ADAMOVSKÝ (203 Czech Republic, belonging to the institution), Kateřina NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Soňa SMETANOVÁ (203 Czech Republic, belonging to the institution) and Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Harmful Algae, AMSTERDAM (NETHERLANDS), Elsevier Science, 2015, 1568-9883
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10511 Environmental sciences
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.664
RIV identification code
RIV/00216224:14310/15:00081599
Organization unit
Faculty of Science
UT WoS
000359027400012
Keywords in English
Retinoic acid receptor; Cyanobacteria; Biomass extracts; All-trans-retinoic acid; 9-cis Retinoic acid; Retinoid-like activity
Tags
International impact, Reviewed
Změněno: 10/7/2017 13:23, RNDr. Mgr. Michal Bittner, Ph.D.
Abstract
V originále
Retinoic acids play a critical role in vital physiological processes and vertebrate development, their derivatives can be produced by some cyanobacterial species into surface waters. This study presents environmentally-relevant information on total retinoid-like activity of field cyanobacterial biomasses and surrounding waters. Intracellular and extracellular levels of total retinoid-like activity and retinoic acids have been investigated at a set of independent sites with the occurrence of water bloom species Microcystis aeruginosa. Twelve samples of biomass and surrounding water from seven localities affected by blooms were studied in comparison with samples from M. aeruginosa laboratory cultures. The method for biomass extraction was optimized and final extracts and samples of surrounding water concentrated by solid phase extraction were assessed using in vitro reporter gene bioassay and chemical analyses for all-trans-retinoic acid (ATRA), 9-cis retinoic acid (9-cis RA) and microcystins RR, LR and YR. Methanol was the most efficient solvent for the extraction of compounds with retinoid-like activity. An in vitro bioassay with the P19/A15 transgenic cell line revealed retinoid-like activity in all cyanobacterial biomasses in the range of 356-2838 ng of retinoid acid equivalents (REQ)/g dry mass (dm), while only three of surrounding water samples exhibited detectable retinoid-like activity, in the range of 12.8-28.7 ng REQ/L. Microcystins were detected in all samples, but they elicited no detectable retinoid-like activity up to 10 mg/L. Chemical analyses detected concentrations up to 340 ng/g dm of all-trans-retinoic acid (ATRA) and 84 ng/g dm 9-cis retinoic acid (9-cis RA) in bloom extracts, and up to 19 ng/L ATRA and 2.2 ng/L 9-cis RA in surrounding water. In most samples, ATRA and 9-cis RA contributed relatively little to the total REQs, which indicates the presence of significant amounts of other compounds with retinoic acid receptor-mediated modes of action.
Links
| GAP503/12/0553, research and development project |
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| LM2011028, research and development project |
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| LO1214, research and development project |
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